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Anti ifnγ af700

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Anti-IFNγ AF700 is a fluorochrome-conjugated antibody that binds to and detects interferon-gamma (IFNγ) in samples. It can be used in various immunoassays and flow cytometry applications to measure the presence and levels of IFNγ.

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Lab products found in correlation

Lsrii flow cytometer, by BD (1 mentions) Anti cd19 pe cy7, by BD (1 mentions) Anti cd3 pe cy7, by BD (1 mentions) Anti cd117 pe, by BD (1 mentions) Anti cd62l apc, by BD (1 mentions) Anti rorγt pe, by BD (1 mentions) Aria 2 cell sorter, by BD (1 mentions) Anti cd49b pe, by BD (1 mentions) Anti t bet apc, by BD (1 mentions) Anti cd45.2 fitc, by BD (1 mentions) Anti gata3 af647, by BD (1 mentions) Anti cd122 pe, by BD (1 mentions) Anti cd25 pacific blue, by BioLegend (1 mentions) Anti cd127 percp cy5, by Thermo Fisher Scientific (1 mentions) Live dead aqua, by Thermo Fisher Scientific (1 mentions) Lsrfortessa, by BD (1 mentions) Anti cd14 bv510, by BioLegend (1 mentions) Anti ccr7 pe cy7, by BioLegend (1 mentions) Anti cd8 bv786 rpa t8, by BD (1 mentions) Anti cd4 apc cy7, by BioLegend (1 mentions)

4 protocols using anti ifnγ af700

1

Multiparametric Flow Cytometry Analysis

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Cells were labelled with flow antibodies for 15 minutes in the dark in PBS containing 2% BSA. Labelled cells were washed twice and resuspended in PBS containing 2% BSA. The prepared samples were analyzed using an LSR-II flow cytometer (BD Bioscience) or sorted using an Aria II cell sorter (BD Bioscience). Anti-CD19-PeCy7 (561739), anti-CD3-PeCy7 (552774), anti-NK1.1-BV421 (562921), anti-NKp46-FITC (560756), anti-NKp46-AF647 (560755), anti-CD49b-PE (553858), anti-CD49a-PerCP-Cy5.5 (564862), anti-CD62L-APC (553152), anti-T-bet-APC (561264), anti-CD45.2-AF700 (560693), anti-CD45.2-FITC (553772), anti-Gata3-AF647 (560068), anti-RORγt-PE (562607), anti-CD127-V450 (561205), anti-CD117-PE (553869), anti-LPAM-1-APC (562376), anti-Flt3-BV421 (566292), anti-Ly-6A/E-APC (565355), and anti-CD122-PE (553362) were purchased from BD Bioscience. Anti-IFN-γ-AF-700 (505823) and anti-CD25-Pacific Blue (102022) were purchased from Biolegend. Anti-CD253-APC (17-5951-82), anti-Eomes-PE (12-4875-82), and anti-CD127-PerCP-Cy5.5 (45-1271-80) were purchased from eBioscience.
Wang Y., Dong W., Zhang Y., Caligiuri M.A, & Yu J. (2018). Dependence of innate lymphoid cell 1 development on NKp46. PLoS Biology, 16(4), e2004867.
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2

Multiparameter Flow Cytometry Characterization

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After stimulation, cells were stained for surface and intracellular markers as
performed previously [23 (link), 24 (link)]. The following antibodies and dyes were
used; Aqua LIVE/DEAD (Invitrogen), anti-CD3 BUV396 (UCHT1; BD Biosciences),
anti-CD19 BV510 (SJ25C1; BioLegend), anti-CD14 BV510 (M5E2; BioLegend), anti-CD4
BUV805 (SK3; BD Biosciences), anti-CD8 BV786 (RPA-T8; BD Biosciences), anti-CCR7
PE/Cy7 (G043H7; BioLegend), anti-CD45RA PE/TR (MEM-56; Invitrogen), anti-KLRG1
eFlour710/PerCP (13F12F2; eBioscience), anti-IFN-γ AF700 (B27;
BioLegend), anti-IL-2 APC/Cy7 (MQ1-17H12; BioLegend), anti-TNF-α Pacific
Blue (MAb11; BioLegend), anti-Perforin-1 PE (B-D48; Cell Sciences),
anti-Granzyme B PE/Cy5.5 (GB11; Invitrogen), anti-T-bet BV605 (4B10; BioLegend),
and anti-Eomesodermin (EOMES) eFlour660 (WD1928; eBioscience). Flow cytometric
analysis was performed on a LSRFortessa (BD Biosciences) and analyzed by Flowjo
v10.6.1. Statistics analysis was done by SPICE V6 [25 (link)] and GraphPad Prism 8.
Jin W., Fang M., Sayin I., Smith C., Hunter J.L., Richardson B., Golden J.B., Haley C., Schmader K.E., Betts M.R., Tyring S.K., Cameron C.M., Cameron M.J, & Canaday D.H. (2023). Differential CD4+ T-Cell Cytokine and Cytotoxic Responses Between Reactivation and Latent Phases of Herpes Zoster Infection. Pathogens and Immunity, 7(2), 171-188.
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3

Comprehensive Immune Cell Profiling

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Cells from mesenteric lymph nodes were isolated and stimulated as previously described (Ramanan et al., 2016 (link)). Stimulated cells were stained with anti-CD45 Pacific Blue, anti-CD3ε FITC, anti CD4 APC-Cy7, and anti-IFNγ AF700 from Biolegend and anti-CD19 PerCP Cy5.5 from eBioscience. Fixation and permeabilization buffers from Biolegend were used for intracellular cytokine staining, and a fixable live/dead stain from Biolegend was used to exclude dead cells. For nuclear staining, unstimulated cells were stained with anti-CD45 Pacific Blue, anti-CD3 FITC, anti-CD4 APC-Cy7, anti-FoxP3 PE-Cy7, and anti-Tbet APC from Biolegend, and anti-CD19 PerCP Cy5.5 and anti-GATA3 PE from eBioscience using the Foxp3 staining kit (eBioscience). Flow cytometric analysis was performed on a CytoFLEX analyzer (Beckman Coulter) and analyzed using FlowJo 10.0.8.
Venzon M., Das R., Luciano D.J., Burnett J., Park H.S., Devlin J.C., Kool E.T., Belasco J.G., Hubbard E.J, & Cadwell K. (2022). Microbial byproducts determine reproductive fitness of free-living and parasitic nematodes. Cell host & microbe, 30(6), 786-797.e8.
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4

Multi-parameter NK Cell Phenotyping

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NK cells were stained with anti-CD56 PE/Cy7, anti-CD16 BV510, anti-CD25 BV421, anti-HLA-DR BV650, anti-CD69 APC (all from BioLegend), Fixable Viability Stain 780 (BD Biosciences), and anti-CD3 BUV395 (BD Biosciences) for 30 min at 4°C, followed by washing in FACS buffer [PBS pH 7.2; 0.5% BSA (Sigma); 2 mM EDTA (Merck)] and fixed with 2% paraformaldehyde (Merck). For the detection of NK cell-expressed cytokines, NK cells were permeabilized and fixed using the Fixation/Permeabilization Solution kit (BD Biosciences) following the manufacturer's protocol and stained with anti-IFNγ AF700 (BioLegend). NK cells were analyzed for marker expression on the LSRFortessa X-20 (BD Biosciences) and analyzed using FlowJo software (Tree Star).
Kroes M.M., Mariman R., Hijdra D., Hamstra H.J., van Boxtel K.J., van Putten J.P., de Wit J, & Pinelli E. (2019). Activation of Human NK Cells by Bordetella pertussis Requires Inflammasome Activation in Macrophages. Frontiers in Immunology, 10, 2030.
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