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2 protocols using phenylpropionitrile

1

Analytical Methods for Glucosinolate Analysis

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Reagents were of analytical purity. Media components (peptone, glucose monohydrate, sodium nitrate, potassium hydrogenphosphate, magnesium sulfate, potassium chloride, ferrous sulfate) were from Reanal (Budapest, Hungary). Allyl isothiocyanate, allyl cyanide, 2-phenylethyl isothiocyanate and phenylpropionitrile and glutathione (reduced form) were from Sigma Aldrich (MO, USA). Pure glucosinolates were from Phytoplan (Germany). LC-MS grade acetonitrile, water and formic acid were purchased from Fisher Scientific (Belgium). Solvents (1-propanol, 2-propanol) and media additives (streptomycin, chloramphenicol and dichloran) were from VWR. Double distilled water was used throughout the study.
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2

Analytical-Grade Reagents for Biochemical Assays

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Reagents were at least of analytical purity. Media components (malt extract, peptone, sodium nitrate, potassium hydrogenphosphate, magnesium sulfate, potassium chloride, ferrous sulfate) were purchased from Reanal (Budapest, Hungary); glucose-monohydrate, streptomycin, chloramphenicol, and dichloran were from VWR (Radnor, PA, USA). Acetone, allyl isothiocyanate, allyl cyanide, 2-phenylethyl isothiocyanate, phenylpropionitrile, dimethyl sulfide, carbon disulfide, methanol, ethyl acetate, methyl acetate, and methyl formate were purchased from Sigma Aldrich (St. Louis, MO, USA). Pure sinigrin was obtained from Phytoplan (Heidelberg, Germany). Type I (18.2 MΩ cm−1) water purified by a Human Zeneer Power I water purification system (Human corporation, Seoul, Korea) was used throughout the study.
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