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Epiquik

Manufactured by Epigentek
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The EpiQuikTM is a laboratory equipment designed for the analysis of epigenetic modifications. It provides a platform for the detection and quantification of various epigenetic markers, such as DNA methylation and histone modifications, in a rapid and efficient manner.

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EpiQuik 8-OHdG DNA Damage Quantification Direct Kit (12 citations) EpiQuik m6A RNA Methylation Quantification kit (colorimetric) (12 citations) EpiQuik CUT&RUN m6A RNA Enrichment Kit (11 citations) EpiQuik™ m6A RNA Methylation Quantitative Kit (8 citations) EpiQuik Chromatin Immunoprecipitation Assay Kit (3 citations) EpiQuik HDAC2 Activity Assay Kit (2 citations) EpiQuik Histone H4 Modification Multiplex Assay Kit (2 citations) EpiQuik m6A RNA Methylation Quantitation Kit (2 citations) EpiQuik MeDIP Ultra Kit (2 citations) EpiQuik m6A RNA Methylation Quantitative Colorimetric kit (2 citations)

2 protocols using epiquik

1

ChIP-Seq of HBx Protein-DNA Interactions

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The chromatin immunoprecipitation assays (ChIP) was performed using the EpiQuikTM chromatin immunoprecipitation kit from Epigentek Group Inc according to the published methods. Protein-DNA complexes were immunoprecipitated with HBx antibodies, with mouse IgG as a negative control antibody and anti-polymerase II as positive control. DNA collected by these antibodies was subjected to PCR analysis, followed by sequencing. Amplification of soluble chromatin prior to immunoprecipitation was used as an input control. All primers were listed in Supplementary Table S2.
Feng G., Li J., Zheng M., Yang Z., Liu Y., Zhang S., Ye L., Zhang W, & Zhang X. (2016). Hepatitis B virus X protein up-regulates C4b-binding protein α through activating transcription factor Sp1 in protection of hepatoma cells from complement attack. Oncotarget, 7(19), 28013-28026.
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2

Colorimetric HDAC Activity Assay

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HDAC enzyme activity was evaluated using the commercial colorimetric assay kit EpiQuik TM (Epigentek, Farmingdale, NY, . Active HDAC's in 5ug nuclear extracts hydrolyze acetylated histone substrates captured on strip wells. Specific antibodies to unacetylated substrates recognize and can be colorimetrically quantified through an ELISAlike reaction. HDAC activity is inversely proportional to the amount of the remaining acetylated histone substrates.
Kao A.C., Chan K.W., Anthony D.C., Lennox B.R, & Burnet P.W. (2019). Prebiotic reduction of brain histone deacetylase (HDAC) activity and olanzapine-mediated weight gain in rats, are acetate independent. Neuropharmacology, 150.
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