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5 protocols using gum arabic

1

Cryoprotectant Storage and Formulation

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CRL (Type VII) was gained from Sigma-Aldrich (St. Louis, Missouri, USA) and stored at 0–4°C [22 ]. Trehalose, DMF, gum arabic, and olive-oil were available from Sinopharm Chemical Reagent Co., Ltd. All other chemicals were analytical-regent-grade.
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2

Charcoal Meal Detoxification Protocol

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An activated carbon solution was prepared by mixing 100 g of gum arabic (Sinopharm Chemical Reagent Co., Ltd., Shanghai, China) with 800 mL of water, boiling the solution until it became transparent, adding 50 g of activated carbon, then boiling the solution again, and cooling the solution to 15 °C. The solution was diluted to 1000 mL and stored at 4 °C until use. After being fed a charcoal meal, the rats were placed in a dry cage box. The feeding time and time of discharge of the first black stool were recorded, and the time until discharge of the first black stool was calculated.
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3

Antioxidant Properties of Tea Tree Oil

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TTO (laboratory extraction). Gelatin (bloom: 100, isoelectric point: 4.72), gum arabic, sodium hydroxide (NaOH), and glacial acetic acid were purchased from Sinopharm Group Chemical Reagent Co. N-Butyl-cyanoacrylate was purchased from the Chongqing Jingu Rubber Industry. DPPH and ABTS were purchased from Shanghai Yuan Ye Biotechnology Co. In addition to TTO, all reagents used in this experiment were of analytical grade and used as received unless stated otherwise. Triple distilled water was used throughout the investigation.
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4

Lipid Encapsulation of Whey and Soy Proteins

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Food-grade TPSO was supplied by Heze Ruipu Peony Industry Technology Development Co., Ltd. (Heze, Shandong, China). The composition of major fatty acids and beneficial components in TPSO was shown in Table S1. Whey protein isolate (WPI, food grade) was purchased from Zhengzhou Weifeng Biotechnology Co., Ltd. (Zhengzhou, Henan, China). Soybean protein isolate (SPI, biological reagent grade), mucin (porcine stomach, biological reagent grade), pepsin (porcine gastric mucosa, 30,000 U/g), bile salt (pig, cholic acid content ≥ 60%), and pancreatin (pancreatic lipase activity ≥ 4000 U/g) were supplied by Yuanye Biological Technology Co., Ltd. (Shanghai, China). Nile red was purchased from Shanghai Macklinn Biochemical Technology Co., Ltd. (Shanghai, China). Gelatin (GE, chemical pure grade), sodium alginate (SA, chemical pure grade), gum arabic (GA, biological reagent grade), sodium carboxymethyl cellulose (CMC-Na, chemical pure grade), sodium benzoate, and other reagents of analytical grade were obtained from Sinopharm Chemical Reagents Co., Ltd. (Shanghai, China).
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5

Stimuli-Responsive Textile Finishing

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Gum arabic, lavender fragrance oil, sodium dodecyl sulfate and gelatin were purchased from SinoPharm Chemical Reagent Co., Ltd. (Shanghai, China). N-isopropylacrylamide (NIPAM), ammonium persulphate (APS), N, N′-methylene bisacrylamide (BIS) and ethanol were purchased from Titan Technology Co., Ltd. (Shanghai, China). These are analytical reagents and were used as received without further purification. Maleic-methyl acrylate copolymer and cotton fabric (5 cm × 5 cm, 140 g/m2) were provided by Shanghai Yichun Industrial Co., Ltd. (Shanghai, China).
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