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General salts

Manufactured by Merck Group
Sourced in United States

General salts are laboratory-grade inorganic compounds commonly used in various scientific and industrial applications. They serve as essential components in chemical reactions, buffers, and other experimental procedures. These salts provide a consistent and reliable source of specific ions or compounds required for a wide range of laboratory activities.

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3 protocols using general salts

1

Isolation and Treatment of Cells

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General salts, collagenase I, papain, LPS, SKF-96365, and 2-aminoethoxydiphenyl borate (2-APB) were purchased from Sigma-Aldrich (USA).
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2

Neuroactive Compound Experiments

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The chemicals and drugs used for this series of studies were purchased from retail distributors. Specifically CAP, AEA, SB366791, RR, TTX and Fura-2 AM were sourced from Tocris. The general salts used for making bath solutions were purchased from Sigma-Aldrich.
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3

Pharmacological manipulation of neural activity

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Drugs were purchased from retail distributers including tetrodotoxin (TTX, 1 μM; Tocris #1069), 2,3-dioxo-6-nitro-7-sulfamoyl-benzoquinoxaline (NBQX, 25 μM; Tocris #0373), D-2-Amino-5-phosphonopentanoic acid (D-AP5, 25 μM; Tocris #0106). These were stored as stock aliquots and diluted in fresh aCSF to their final concentration the day of recording. The general salts used for making bath solutions were purchased from Sigma-Aldrich. All drugs were delivered via bath perfusion at a flow rate of ~2 mL / min and slices were allowed to fully recover before the start of recording.
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