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2 protocols using magnesium 2 chloride hexahydrate

1

Determination of Heavy Metal Ions

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All reagents were analytical grade. Ultrapure water (18.2 MΩ·cm) from a Mill-Q system (Merck Millipore, Darmstadt, Germany) was used throughout. Laboratory containers were rinsed with H2O prior to use. Standard solutions of all metals (lead(II) nitrate, cadmium(II) nitrate tetrahydrate, potassium dichromate(VI), iron(III) chloride hexahydrate, nickel(II) sulfate hexahydrate, copper(II) sulfate pentahydrate, manganese(II) chloride tetrahydrate, magnesium(II) chloride hexahydrate, iron(II) sulfate heptahydrate, aluminum(III) sulfate hydrate, barium(II) chloride, vanadium(III) chloride, and cobalt(II) sulfate pentahydrate) were purchased from Sigma-Aldrich (St. Louis, MO, USA). L-ascorbic acid (97%), L-cysteine, Tris base (99.9%), bathophenanthroline (97%), dimethylglyoxime (99%), dithiooxamide (98.5%), and sodium fluoride were purchased from Sigma-Aldrich. Sodium acetate, ammonium acetate, hydrochloric acid, sodium hydroxide, and glacial acetic acid were obtained from Fisher Scientific (Pittsburgh, PA, USA). Whatman (grade 1) filter paper was purchased from Apollo Presentation Products (Booneville, MS, USA).
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2

Trace Metal Analysis Workflow

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4-Phenylazo-m-phenylenediamine (Chrysoidine G, CG), lithium chloride, iron(II) chloride tetrahydrate, iron(III) chloride hexahydrate, magnesium(II) chloride hexahydrate, manganese(II) chloride tetrahydrate, and aluminum(III) chloride hexahydrate was purchased from Sigma-Aldrich Chemicals (St. Louis, MO, USA). Cobalt standard solutions were purchased from Kanto Chemical Co., Inc. (Tokyo, Japan). Standard copper, zinc, arsenic, cadmium, and mercury solutions were purchased from CPI International, Co. (Santa Rosa, CA, USA). All reagents and chemicals were of analytical grade and were prepared using highly pure water with a resistivity of 18 MΩ·cm.
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