The major independent variable in this study was the fasting glucose level, which was measured within 2 days before surgery. In this study, plasma was collected from patients during morning fasting for blood glucose measurement. Plasma from all patients was tested on the Architect ci 16200 system (Abbott Laboratories). Thresholds were determined based on the 25th and 75th percentiles of blood glucose levels.
Architect ci 16200 system
Manufactured by Abbott
Sourced in United States
The Architect ci 16200 system is an automated clinical chemistry analyzer developed by Abbott. It is designed to perform a variety of in vitro diagnostic tests on various biological samples. The system automates the analysis process, offering efficient and reliable results.
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Lab products found in correlation
4 protocols using architect ci 16200 system
1
Fasting Glucose Levels and Surgery
2
Comprehensive blood analysis protocol
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Whole blood analysis was performed using the scil Vet abc Plus+ hematology analyzer (scil animal care company GmbH, Viernheim, Germany) to quantify red blood cell count, platelet count, hematocrit, hemoglobin, and mean corpuscular volume. Plasma was prepared by centrifugation of heparinized whole blood for 10 min at 3000 g. Creatinine, urea, sodium, and potassium were quantified in plasma samples with the ARCHITECT™ ci16200 System (Abbott, Chicago, IL, USA). NGAL was quantified in plasma samples by ELISA (Cat. No. 443707; BioLegend®, Inc., San Diego, CA, USA). cTnI levels were determined in plasma samples by ELISA (CTNI-1-HSP; Life Diagnostics, Inc., West Chester, PA, USA). Quantification of brain natriuretic peptide (BNP) was performed by an enzyme immunoassay (RAB0386; Sigma-Aldrich, St. Louis, MO, USA). In some cases, not all measurements were possible due to too little plasma volumes.
3
Preoperative Plasma Glucose Levels
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The main point of interest in this study was the fasting glucose level 2 days before surgery or closest to the time of surgery. Plasma was collected in the morning from all patients and the same testing equipment was used. According to the manufacturer's instructions, a fully automated particle-enhanced immunoturbidimetric assay was used on the Architect ci 16200 system (Abbott Lab, Illinois, USA) and plasma glucose levels were measured using the hexokinase enzymatic method. Information on standard biosecurity and institutional safety procedures were followed throughout the blood collection process.
4
Serum Creatinine and Cystatin C Measurements
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Venous blood samples were collected between 0830 and 1030 h after overnight fasting at baseline and were stored at − 80 °C until assessment. In 2005, serum creatinine was measured from baseline serum samples using an isotope dilution mass spectrometry (IDMS) traceable Jaffe kinetic assay for creatinine (Hitachi 917 auto-analyzer, Roche Diagnostics GmbH, Mannheim Germany), whereas 5-year serum creatinine was measured in 2012 on the Architect ci16200 analyzer (Abbott Laboratories, Abbott Park, Ill., USA)26 (link). Serum cystatin C was measured using a fully automated particle-enhanced immunoturbidimetric assay with Sentinel Diagnostics reagents (Sentinel CH, Milan, Italy) on the Architect ci 16200 System (Abbott Laboratories, Abbott Park, Ill., USA) according to the manufacturer’s instructions. The correlation coefficient (r2) between the machines was 0.998 with a Passing and Bablok slope of 0.966 and a Passing and Bablok intercept of 6.16 (n = 37)26 (link).
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