Ec plan neofluar lens

Manufactured by Zeiss

Sourced in Germany

The EC Plan-Neofluar lens is a high-quality microscope objective lens designed and manufactured by Zeiss. It is characterized by its excellent optical performance, which includes high contrast, resolution, and flat field of view. The lens is suitable for a variety of microscopy applications and is compatible with Zeiss microscope systems.

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Lab products found in correlation

Live dead staining kit, by Thermo Fisher Scientific (1 mentions) Gaasp detector, by Zeiss (1 mentions) Lsm 880 airyscan, by Zeiss (1 mentions) Airyscan module, by Zeiss (1 mentions) Mab377, by Merck Group (1 mentions)

4 protocols using ec plan neofluar lens

Live/Dead Cell Viability Assay on Films

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Confluence and cell viability on the surface-seeded films with a total area of 6 cm² were investigated by differential staining of living and dead cells with a Live/Dead staining kit (Invitrogen, Waltham, MA, USA). Murine fibroblasts of the 3T3 line were stained after 76 h of incubation on the surface of the films. A scanning laser confocal microscope LSM 880 Airyscan (Carl Zeiss, Oberkochen, Germany) equipped with an AiryScan module and a GaAsP detector (Carl Zeiss, Oberkochen, Germany) was used to visualize green living and red dead cells. Z scans were obtained using an EC Plan-Neofluar lens (Carl Zeiss, Oberkochen, Germany) and lasers with wavelengths of 488 and 561 nm.

Buinov A.S., Gafarova E.R., Grebenik E.A., Bardakova K.N., Kholkhoev B.C., Veryasova N.N., Nikitin P.V., Kosheleva N.V., Shavkuta B.S., Kuryanova A.S., Burdukovskii V.F, & Timashev P.S. (2022). Fabrication of Conductive Tissue Engineering Nanocomposite Films Based on Chitosan and Surfactant-Stabilized Graphene Dispersions. Polymers, 14(18), 3792.

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Live Imaging of Centrosomal Dynamics

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Embryos for live imaging experiments were obtained by dissecting gravid adult hermaphrodites in M9 buffer (42 mM Na2HPO4, 22 mM KH2PO4, 86 mM NaCl, and 1mM MgSO4 or a 100X 1.3 EC Plan Neofluar lens (Zeiss), in a temperature-controlled room at 20˚C. Images of centrosomal fluorescence and chromosome segregation were acquired every 30 or 60s by collecting 9-12 z-planes at 1.0 µm intervals or 11 z-planes at 1.5 µm intervals without binning. Imaging was initiated in one-cell embryos between centrosome separation and pronuclear meeting and was terminated after initiation of cytokinesis.

Ohta M., Zhao Z., Wu D., Wang S., Loza J., Gómez-Cavazos J.S., Desai A., & Oegema K. (2020). Polo-like kinase 1 independently controls microtubule-nucleating capacity and size of the centrosome.

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Quantitative Histological Analysis of MRI Findings

Cited 1 time

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Histological examinations were performed on the specimens after the MRI
scans. Coronal 8-μm thick slices were stained for the demonstration of
myelin using a standard Luxol Fast Blue (LFB) protocol. Adjacent sections were
stained by means of immunocytochemistry for the demonstration neuronal cell
bodies using antibodies directed against the neuronal nuclear antigen (NeuN)
(MAB377, lot 2967854, Millipore, Burlington, MA). Slides were imaged using
Axioscop2 FSmot optical microscope with EC PlanNeofluar Zeiss lens at 20×
magnification, 0.3 aperture under the same settings and light conditions. In
order to quantitatively compare histology results with MRI results (Kamagata et al. 2017 (link); Sato et al. 2017 (link)), the intensity of LFB/NeuN was
measured based on the transmitted light intensity (luminosity) by the equation
below:

I_{i i} = \frac{(m a x l u m - l u m R O I)}{m a x l u m}

Where ii stands for LFB or NeuN,
maxlum is the maximum luminosity of the image,
lumROI represents the luminosity of a specific ROI. The
I_ii is normalized by the
maxlum to [0 1], the same range as NODDI metrics.

Wang N., Zhang J., Cofer G., Qi Y., Anderson R.J., White L.E, & Johnson G.A. (2019). Neurite Orientation Dispersion and Density Imaging of Mouse Brain Microstructure. Brain structure & function, 224(5), 1797-1813.

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Histological Assessment of Ex Vivo MRI Samples

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Histological examinations were performed after the ex vivo MRI scans. Coronal 6-μm thick slices were stained with myelin staining agent (LFB – Luxol Fast Blue) and iron staining agent (PPB – Perl’s Prussian Blue). As a control for iron staining, one slice of human liver tissue was also stained with PPB with the same protocol simultaneously. Slides were imaged using Axioscop2 FSmot optical microscope with EC PlanNeofluar Zeiss lens at 10x magnification, 0.3 aperture under the same settings and light conditions. The histological slices were evaluated by two pathologists with more than 5 years of experience.

Wang N., Zhuang J., Wie H., Dibb R., Qi Y, & Liu C. (2019). Probing demyelination and remyelination of cuprizone mice model using multimodality MRI. Journal of magnetic resonance imaging : JMRI, 50(6), 1852-1865.

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