The cells were collected and incubated with the corresponding antibodies (PE/Cyanine7 anti-human CD147 antibody, 306,216, BioLegend; FITC anti-human CD3 antibody, 300,406, BioLegend; APC/Cyanine7 anti-human CD4 antibody, 317,418, BioLegend; PerCP/Cyanine5.5 anti-human CD8a antibody, 301,032, BioLegend; FITC-Labeled Recombinant Protein L, RPL-PF141, ACRO Biosystems; PE anti-human CD25 antibody, 302,606, BioLegend; APC anti-human CD69 antibody, 310,910, BioLegend; APC anti-human CD107a antibody, 328,619, BioLegend; PE/Cyanine7 anti-human PD-1 antibody, 329,917, BioLegend; PerCP/Cyanine5.5 anti-human CD8a antibody, 301,032, BioLegend; PE-anti human IL-2 antibody, 500,307, BioLegend; PE/Cyanine7 anti-human IFN-γ antibody, 502,528, BioLegend; APC anti-human TNF-α antibody, 502,912, BioLegend) at 4 °C for 30 min. After washed with PBS, all samples were detected by FACS Aria instrument (BD Biosciences) and data were analyzed with FlowJo7.6 software (Tree Star).
Pe cyanine7 anti human ifn γ antibody
Manufactured by BioLegend
Sourced in United States
The PE/Cyanine7 anti-human IFN-γ antibody is a dual-labeled monoclonal antibody that specifically binds to human interferon-gamma (IFN-γ). The antibody is conjugated with both phycoerythrin (PE) and Cyanine7 fluorescent dyes, allowing for detection and analysis of IFN-γ-producing cells using flow cytometry.
Automatically generated - may contain errors
Lab products found in correlation
Facsaria instrument, by BD (1 mentions)
Fitc anti human cd3 antibody, by BioLegend (1 mentions)
Pe anti human cd25 antibody, by BioLegend (1 mentions)
Apc anti human cd69 antibody, by BioLegend (1 mentions)
Percp cyanine5.5 anti human cd8a antibody, by BioLegend (1 mentions)
Apc anti human tnf α antibody, by BioLegend (1 mentions)
Facscanto 2, by BD (1 mentions)
Zombie nir fixable viability kit, by BioLegend (1 mentions)
Pe anti human cd16 antibody, by BioLegend (1 mentions)
Pe mouse anti human igg, by BD (1 mentions)
Pe anti human cd19 antibody, by BioLegend (1 mentions)
3 protocols using pe cyanine7 anti human ifn γ antibody
1
Multiparametric Immune Cell Profiling
2
Multiparametric Flow Cytometry Analysis
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Cells were stained with FITC anti-human CD8 Antibody, PE/Cyanine7 anti-mouse CD8 Antibody, PE/Cyanine7 anti-human IFN-γ Antibody and PerCP/Cyanine5.5 anti-mouse IFN-γ Antibody according to the manufacturer’s instructions (BioLegend, USA). Among them, IFN-γwere used for intracellular staining. Then, the samples were collected by flow cytometry (BD FACSCanto II, USA) according to the manufacturer’s instructions, and data analysis was conducted with FlowJO software (version 10.0, Tree Star, Inc. Ashland, OR, USA).
3
Multiparametric Immune Cell Analysis
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PE mouse anti-human IgG and APC-H7 mouse anti-human CD3 were purchased from BD (Franklin Lakes, NJ, USA). Goat anti-human IgG1 PE was purchased from Thermo Fisher Scientific (Waltham, MA, USA). PE/Cyanine7 anti-human IFN-γ antibody, PE anti-human TNF-α antibody, APC anti-human CD107a (LAMP-1) antibody, APC anti-human CD56 (NCAM) antibody, PE anti-human CD16 antibody, PE anti-human CD335 (NKp46) antibody (9E2), Brilliant Violet 421™ anti-human CD45 antibody, PE/Cyanine7 anti-human CD14 antibody, PE anti-human CD19 antibody, and Zombie NIR™ Fixable Viability Kit were purchased from Biolegend (San Diego, CA, USA).
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