Garlic samples were collected form Pizhou, China (abbreviation: PW), and cultivated in the test farm (Xuzhou city, Jiangsu province) in an area of more than 100m2. Vernier calipers were used to measure garlic leaf length, width and thickness at maturity in plants with a similar phenotype. Samples were obtained at 0, 3, 6 and 12 h after wounding. Garlic root, clove, inner bud and sprout samples were also collected (Fig. 1 a) then immediately frozen in liquid nitrogen and stored at 80 °C until use. Total RNA was extracted using a Plant Total RNA Isolation Kit(Vazyme, China) according to the manufacturer. DNase I (Vazyme, China) was added to the mixture to prevent DNA contamination. Purified RNA was analysed by 1% agarose gel electrophoresis and the quality of total RNAs was confirmed using an RNA 6000 Nano LabChip kit (Agilent, Santa Clara, CA, USA) with an RNA integrity number > 7.0.
Plant total rna isolation kit
Manufactured by Vazyme
Sourced in China
The Plant Total RNA Isolation Kit is a product designed to efficiently extract and purify total RNA from a variety of plant samples. The kit utilizes a rapid and effective method to isolate high-quality RNA suitable for downstream applications such as RT-PCR, Northern blotting, and gene expression analysis.
Automatically generated - may contain errors
Lab products found in correlation
Nanodrop nd 1000 spectrophotometer, by Thermo Fisher Scientific (3 mentions)
Dnase 1, by Vazyme (2 mentions)
Rna 6000 nano labchip kit, by Agilent Technologies (2 mentions)
Plant genomic dna isolation kit, by Tsingke (1 mentions)
Hiscript q rt supermix for qpcr gdna wiper, by Vazyme (1 mentions)
Plant genomic dna kit, by Tiangen Biotech (1 mentions)
Nanometer photometer, by Implen (1 mentions)
5 protocols using plant total rna isolation kit
1
Garlic Tissue Sampling and RNA Extraction
2
RNA Extraction and cDNA Synthesis
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The tea leaves were divided into two parts along the main vein of the leaves. Half of them were used to extract gDNA (RNA free) using a Plant Genomic DNA Isolation Kit (Tsingke, Beijing, China). The total RNA was extracted from the other half of the samples using a Plant Total RNA Isolation Kit (Vazyme, Nanjing, China). A total of 1% agarose gel electrophoresis was used to check the integrity of total RNA. The concentration of total RNA was measured with a NanoDrop ND 1000 spectrophotometer (Thermo Fisher Scientific, Waltham, MA, USA). A total of 1–2 μg of the total RNA was used to synthesize the first-strand complementary DNA (cDNA) with HiScript Q RT SuperMix for qPCR (+gDNA wiper) (Vazyme, Nanjing, China). Specific primers were used to amplify genes containing RNA editing sites using the gDNA and cDNA as templates, respectively (Table S1 ).
3
Plant DNA and RNA Extraction
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P. tabuliformis genomic DNA was extracted from the needles following the protocol of the Plant Genomic DNA kit (TIANGEN, Beijing, China). Total RNA from different tissues of P. tabuliformis or Arabidopsis was extracted following the protocol of the Vazyme Plant Total RNA Isolation Kit (Vazyme, Nanjing, China) and stored at −80°C. 1 μg total RNA was used for first-strand cDNA samples generated by the Vazyme kit (Vazyme, Nanjing, China).
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P. tabuliformis genomic DNA was extracted from the needles following the protocol of the Plant Genomic DNA kit (TIANGEN, Beijing, China). Total RNA from different tissues of P. tabuliformis or Arabidopsis was extracted following the protocol of the Vazyme Plant Total RNA Isolation Kit (Vazyme, Nanjing, China) and stored at −80°C. 1 μg total RNA was used for first-strand cDNA samples generated by the Vazyme kit (Vazyme, Nanjing, China).
4
Quantitative Gene Expression Analysis of Broccoli Sprouts
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Total RNA from broccoli sprouts was extracted using a plant Total RNA isolation kit (Vazyme). cDNA was synthesized using the R223-01 HiScript® ii Q RT SuperMix for qPCR (+ gDNA WIper) kit provided by Vazyme. The sequence-specific primers were listed in Table 1 . The reaction was performed using the Q712-02 Cham QTM SYBR® qPCR Master Mix kit and ABI Prism 7500 (Vazyme) Fast qPCR instrument provided by Vazyme. The synthesized cDNA was used as the template for qPCR amplification. After qPCR was completed, the relative gene expression was calculated by 2−ΔΔCT method.
5
Characterization of Garlic Transcriptome
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Garlic samples were collected form Pizhou, China (abbreviation: PW), and cultivated in the test farm (Xuzhou city, Jiangsu province) in an area of more than 100m 2 . Vernier calipers were used to measure garlic leaf length, width and thickness at maturity in plants with a similar phenotype. Samples were obtained at 0, 3, 6 and 12h after wounding. Garlic root, clove, inner bud and sprout samples were also collected (Fig. 1a) then immediately frozen in liquid nitrogen and stored at 80 ℃ until use. Total RNA was extracted using a Plant Total RNA Isolation Kit(Vazyme, China) according to the manufacturer. DNase I (Vazyme, China) was added to the mixture to prevent DNA contamination. Puri ed RNA was analysed by 1% agarose gel electrophoresis and the quality of total RNAs was con rmed using an RNA 6000 Nano LabChip kit (Agilent, Santa Clara, CA, USA) with an RNA integrity number > 7.0.quantitatively detected by using an Implen nanometer photometer using with Nanodrop with and an RNA integrity number > 7.0.
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