Log In Sign up
The largest database of trusted experimental protocols

Fluorchem sp digital imaging system

Manufactured by Bio-Techne
Visit Supplier
Sourced in United States

The FluorChem SP Digital Imaging System is a compact, high-performance imaging platform designed for a variety of applications in life science research. It captures high-quality digital images of chemiluminescent, fluorescent, and colorimetric samples. The system features a sensitive CCD camera, precision optics, and a user-friendly software interface for image capture and analysis.

Automatically generated - may contain errors

Lab products found in correlation

Superscript 2 kit, by Thermo Fisher Scientific (1 mentions) Gelred, by Biotium (1 mentions) 2720 thermal cycler, by Thermo Fisher Scientific (1 mentions) Rneasy kit, by Qiagen (1 mentions) Hotstarttaq master mix, by Qiagen (1 mentions) Filter paper, by Thermo Fisher Scientific (1 mentions)

2 protocols using fluorchem sp digital imaging system

1

Quantitative RT-PCR Protocol for Gene Expression Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols
mRNA was isolated with an RNeasy Kit (Qiagen, 74104) and subjected to RT-PCR with the Superscript II kit (Invitrogen). The total volume of the PCR reaction was 25.0 µl in each tube, which contained 12.5 μl Qiagen HotStartTaq Mastermix, 2.0 µl 4.0 μM primers, the appropriate volume of cDNA, and ultra-pure water to bring the final volume to 25.0 µl. Samples were loaded into a 2720 Thermal Cycler (Applied Biosystems) and specific PCR conditions were used based on the primer composition. The PCR product was mixed with 2.0 µl 6x loading buffer (Bioline, Bio-37068) and 0.5 µl Gel Red (Biotium, 41003), then loaded into the well for agarose gel electrophoresis. The gel was photographed under UV light using the FluorChem SP Digital Imaging System (Alpha Innotech Corporation, San Leandro, California, USA). Fluorescence density of each PCR-amplified band was normalized with the corresponding value of the GAPDH cDNA band.
Alshehri B., Pagnin M., Lee J.Y., Petratos S, & Richardson S.J. (2020). The Role of Transthyretin in Oligodendrocyte Development. Scientific Reports, 10, 4189.
+ Open protocol
+ Expand
2

Bladder Function Evaluation via VSA

Check if the same lab product or an alternative is used in the 5 most similar protocols
The delivery of therapeutic compounds via intravesical delivery of in situ gelling polymers to the bladder can potentially impact bladder function by occupying volume, affecting bladder elasticity, or obstructing clearance through the urethra. Bladder function was assessed using a void spot assay (VSA). Mice were placed into individual wire mesh-bottomed cages over filter paper (ThermoFisher Scientific, Rockford, IL) for 4 hr. in a quiet room during the middle of their day cycle. The filter paper was collected, dried, and then imaged on an Alpha Innotech FluorChem SP Digital Imaging System under ultraviolet light at 365 nm. The area and number of void spots were calculated by analyzing the images using ImageJ. Volume was calculated from the area of the spots compared to a calibration curve. Spots under 6.6 mm2, corresponding to a volume of 0.5 μL, were excluded from consideration. Mice that did not have any voiding events during the test period were excluded from calculation of average voiding volume.
Jensen M.M., Jia W., Schults A.J., Isaacson K.J., Steinhauff D., Green B., Barber Z., Cappello J., Ghandehari H, & Oottamasathien S. (2019). Temperature-Responsive Silk-Elastinlike Protein Polymer Enhancement of Intravesical Drug Delivery of a Therapeutic Glycosaminoglycan for Treatment of Interstitial Cystitis/Painful Bladder Syndrome. Biomaterials, 217, 119293.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!