Graphite powder (average particle size of 50 μm) was received from Merck Millipore (Darmstadt, Germany) and the epoxy resin EpoTek H77 with the corresponding hardener was supplied by Epoxy Technologies (Billerica, MA, USA). Nanodiamonds (>87%) were obtained from PlasmaChem (Berlin, Germany). Potassium chloride (99.5%), potassium ferrocyanide trihydrate (>99%), potassium ferricyanide (III) (99%), nitric acid (99.5%), potassium nitrate (99%), potassium hydrogenphosphate (99.5%), potassium dihidrogenpohsphate (99.5%), silver nitrate (≥99%), gold chloride trihydrate (≥99.9%), sodium chloride (99.5%), sodium borohydride, chloroauric acid (≤99.9%), hydrogen peroxide (30%), glucose oxidase VII from Aspergillus niger (174,400 units/g), D-(+)-Glucose (≥99.5%), and bovine serum albumin, all of them were supplied by Sigma-Aldrich (St. Louis, MO, USA). Phosphate buffers were prepared from the potassium hydrogenphosphate (K2HPO4) and dihydrogenphosphate (KH2PO4) salts in Milli-Q water (Millipore, Billerica, MA, USA). All the dissolutions were prepared using deionized water from the Milli-Q system (Millipore, Billerica, MA, USA) with a resistivity value of 18.2 MΩ·cm.
Potassium ferrocyanide trihydrate
Manufactured by Merck Group
Sourced in United States
Potassium ferrocyanide trihydrate is a crystalline chemical compound with the formula K4[Fe(CN)6]·3H2O. It is a yellow, water-soluble salt that is commonly used in various laboratory applications, including as a staining agent, precipitating agent, and analytical reagent.
Automatically generated - may contain errors
Lab products found in correlation
Hydrochloric acid (hcl), by Merck Group (3 mentions)
Potassium chloride (kcl), by Merck Group (2 mentions)
Sodium chloride (nacl), by Merck Group (2 mentions)
D glucose, by Merck Group (2 mentions)
Sucrose, by Merck Group (2 mentions)
Potassium ferricyanide, by Merck Group (2 mentions)
Potassium hydrogen phosphate, by Merck Group (1 mentions)
Chloroauric acid, by Merck Group (1 mentions)
Potassium ferricyanide 3, by Merck Group (1 mentions)
Milli q water, by Merck Group (1 mentions)
Milli q system, by Merck Group (1 mentions)
Sodium borohydride, by Merck Group (1 mentions)
Hydrogen peroxide, by Merck Group (1 mentions)
Bovine serum albumin (bsa), by Merck Group (1 mentions)
Silver nitrate, by Merck Group (1 mentions)
Nitric acid, by Merck Group (1 mentions)
Potassium nitrate, by Merck Group (1 mentions)
Gold chloride trihydrate, by Merck Group (1 mentions)
Graphite powder, by Merck Group (1 mentions)
Hemoglobin, by Merck Group (1 mentions)
9 protocols using potassium ferrocyanide trihydrate
1
Characterization of Graphite-Epoxy Nanocomposites
2
Glucose Oxidase-Based Electrochemical Sensing
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Glucose oxidase from Aspergillus niger (>125U), titanium aluminium carbide (Ti3AlC2) powder, potassium ferricyanide (K3[Fe(CN)6]), potassium ferrocyanide trihydrate (K4[Fe(CN)6]·3H2O), iron (III) chloride (FeCl3), potassium chloride (KCl), hydrochloric acid (HCl), lithium fluoride (LiF), D-(+)-glucose, Nafion 117 (~5%), sodium di-hydrogen phosphate monohydrate (NaH2PO4·H2O), di-sodium hydrogen phosphate dihydrate (Na2HPO4·2H2O), sodium chloride (NaCl), ascorbic acid, uric acid, and hemoglobin were obtained from Sigma-Aldrich (St. Louis, MO, USA). Whatman No. 1 filter paper was purchased from Whatman International Ltd. (Maidstone, England) and A4 230-g card paper was purchased in Bangkok, Thailand. In this work, total amounts of 0.2 mM stock solution of glucose were prepared by dissolving D-(+)-glucose in 0.1 M PBS (pH 7.4). The solution of 10 mg/mL glucose oxidase enzyme was prepared by dissolving it in 50 mM sodium acetate (pH 5.1). The deionized water used in this study was obtained in the laboratory by using ELGA LabWater purification system (0.067 μS/cm conductivity, resistivity of 18 MΩ.cm at 25 °C).
3
Prussian Blue Staining for Cerebral Microhemorrhages
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Brains were sectioned into 40-µm coronal sections using a freezing microtome. Sections were stained with Prussian blue to detect hemosiderin, a marker of CMHs, using 10% hydrochloric acid and 5% potassium ferrocyanide trihydrate (Sigma, St. Louis, MO) for 30 min and rinsed in deionized water. Sections were then counterstained with Nuclear Fast Red (Sigma, St. Louis, MO) for 5 min, rinsed in deionized water, dehydrated, and mounted on a glass slide.
4
Synthesis and Characterization of Gold Nanoparticles
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Tetrachloroauric acid, sodium citrate tribasic dihydrate, potassium ferrocyanide trihydrate, casein, fructose, sucrose, glycine and L-lysine were purchased from Sigma-Aldrich (St. Louis, MO, USA). Potassium ferricyanide, bovine serum albumin (BSA), sodium chloride and agar were purchased from ITW Reagents (Glenview, IL, USA). BSA-Alexa FluorTM 647 conjugate was purchased from Thermo Fisher Scientific (Waltham, MA, USA). All the chemicals were of analytical grade and were used as received without any purification steps. All the solutions were prepared in deionizedwater unless otherwise stated.
5
Prussian Blue Histochemistry of Spleen
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Paraffin-embedded
sections of spleens were sectioned at 5 μm
and then dehydrated as described above. The sections were stained
with a freshly prepared mixture of aqueous solutions of 20% hydrochloric
acid and 10% potassium ferrocyanide trihydrate (Sigma-Aldrich, UK)
at a volume ratio of 1:1, followed by counter staining with nuclear
fast red 0.2% aqueous solution. The slides were dehydrated through
95% and 100% ethanol solutions, respectively, with two consecutive
changes for each concentration. They were then coverslipped with resinous
mounting media and left to dry overnight. Images were collected as
described above. The percentage of blue pigmentation was analyzed
by Fiji/ImageJ software (version 1.5c; National Institutes of Health,
USA). This was obtained from three to four images from each mouse
captured at 20× after setting a threshold (n = 3 mice per group).
sections of spleens were sectioned at 5 μm
and then dehydrated as described above. The sections were stained
with a freshly prepared mixture of aqueous solutions of 20% hydrochloric
acid and 10% potassium ferrocyanide trihydrate (Sigma-Aldrich, UK)
at a volume ratio of 1:1, followed by counter staining with nuclear
fast red 0.2% aqueous solution. The slides were dehydrated through
95% and 100% ethanol solutions, respectively, with two consecutive
changes for each concentration. They were then coverslipped with resinous
mounting media and left to dry overnight. Images were collected as
described above. The percentage of blue pigmentation was analyzed
by Fiji/ImageJ software (version 1.5c; National Institutes of Health,
USA). This was obtained from three to four images from each mouse
captured at 20× after setting a threshold (n = 3 mice per group).
6
Larval Tissue Staining Protocol
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3rd instar fly larvae were dissected in PBS and fixed for 15 minutes with PBS containing 0.4% glutaraldehyde (Sigma-Aldrich) and 1 mM MgCl2 (Sigma-Aldrich). The samples were washed with PBS and incubated with a freshly prepared staining solution containing 5 mg/ml X-gal (5-Bromo-4-chloro-3-indolyl-β-D-galactopyranoside), 5 mM potassium ferrocyanide trihydrate (Sigma-Aldrich), 5 mM potassium ferrocyanide crystalline (Sigma-Aldrich) and 2 mM MgCl2 in PBS at 37°C. After washing with PBS, the samples were mounted using Mowiol (Sigma) and imaged with brightfield microscopy (Leica, Wetzlar, Germany).
7
Prebiotic and Probiotic Compound Analysis
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5-Caffeoylquinic acid (5-CQA), caffeine, zinc acetate, potassium ferrocyanide trihydrate, glacial acetic acid, sucrose, SDS, paraformaldehyde and lysosyme were obtained from Sigma-Aldrich. Ethanol, HPLC grade; mEthanol, acetonitrile and hydrochloric acid, liquid chromatography -MS (LC-MS) grade; formic acid, acetonitrile, mEthanol and water were obtained from Fisher Scientific. Peptone water, yeast extract, NaCl, dipotassium phosphate, monopotassium phosphate, sodium bicarbonate, magnesium sulphate heptahydrate, calcium chloride hexahydrate, Tween-80, haemin, phylloquinone (vitamin K 1 ), L-cystine, bile salts, resazurin, PBS tablets were obtained from Oxoid Limited. ProLong w Gold antifade reagent was obtained from Invitrogen. Raftilose P95 FOS (a positive prebiotic control) was purchased from Orafti. Oligonucleotide probes (EUB338/ II/III, CHIS150, LAB158, BAC303, EREC482 and BIF164) were commercially synthesised and labelled with Cy3 by MWG Biotech Limited. Aquasonic w 100 ultrasound gel was purchased from Parker Laboratories Inc. Deionised water was obtained using a Purite dispenser.
8
Electrochemical Sensor Fabrication Protocol
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Zinc nitrate hexahydrate (Zn(NO3)2, 6H2O), 2-methylimidazole (Hmim, C4H6N2), poly(3,4-ethylenedioxythiophene) polystyrene sulfonate (PEDOT:PSS), creatine, phosphate-buffered saline 7.4 (PBS 7.4) tablets, Potassium ferrocyanide trihydrate (K4Fe(CN)6·3H2O), potassium ferricyanide (K3Fe(CN)6), potassium chloride (KCl), and Polydimethylsiloxane (PDMS) were purchased from Merck, Taiwan. Deionized water (DI) was accessed from a Millipore Milli-Q plus 185 purification system with a resistivity of 18.2 MΩ.
9
Standardized Urea Quantification Assay
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All chemicals used were of analytical reagent grade except PR, which was of laboratory grade. Urease, glycerol, sodium hydroxide (NaOH), p‐dimethyl amino benzaldehyde (DMAB), ethanol, hydrochloric acid, acetic acid, potassium dihydrogen phosphate, dipotassium hydrogen phosphate, potassium ferrocyanide trihydrate, and 3′, 3″‐dibromothymolsulfonphthalein (BTB) were purchased from Merck (Darmstadt, Germany). Activated charcoal was purchased from Sigma‐Aldrich (MO, USA). Phenolsulfonphthalein (PR) was purchased from Ajax Finechem Pty Ltd (NSW, Australia). Urea was purchased from Sigma‐Aldrich (Steinheim, Germany). Zinc acetate dehydrate was obtained from Loba Chemie (Mumbai, India). All stock solutions were prepared in deionized (DI) water.
A phosphate buffer solution (pH 7.0) was prepared by dissolving 3.403 g of anhydrous potassium dihydrogen phosphate and 4.355 g of anhydrous dipotassium phosphate in 900 mL of DI water. Then, the pH of the buffer solution was adjusted to 7.0 and the final volume was adjusted to 1 L with DI water.
A phosphate buffer solution (pH 7.0) was prepared by dissolving 3.403 g of anhydrous potassium dihydrogen phosphate and 4.355 g of anhydrous dipotassium phosphate in 900 mL of DI water. Then, the pH of the buffer solution was adjusted to 7.0 and the final volume was adjusted to 1 L with DI water.
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