Human HCC cell lines (Huh7, HCCLM6, MHCC97-L and MHCC97-H, Cobioer Biosciences, Nanjing, China) and THLE-2 (Bluefbio, Shanghai, China) were maintained in DMEM medium (Gibco, Carlsbad, CA, USA) plus 10% FBS (Gibco) and 1% penicillin–streptomycin mixture (Solarbio, Beijing, China) in a moist circumstance under 37 °C with 5% CO2.
Mhcc97l
Manufactured by Cobioer Biosciences
Sourced in China
The MHCC97L is a cell line used in cell biology research. It is derived from a human hepatocellular carcinoma. The MHCC97L cell line is commonly used for in vitro studies.
Automatically generated - may contain errors
Lab products found in correlation
Mhcc97h, by Cobioer Biosciences (4 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (3 mentions)
Dmem medium, by Thermo Fisher Scientific (2 mentions)
Penicillin streptomycin mixture, by Solarbio (1 mentions)
Hepg2, by Cell Resource Center (1 mentions)
Smmc 7721, by Cell Resource Center (1 mentions)
Plc prf 5, by Cell Resource Center (1 mentions)
Qgy 7703, by Cell Resource Center (1 mentions)
Qsg 7701, by Cell Resource Center (1 mentions)
Hcclm3, by Cell Resource Center (1 mentions)
Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (1 mentions)
Bel7402, by Cobioer Biosciences (1 mentions)
Hepg2, by Cobioer Biosciences (1 mentions)
4 protocols using mhcc97l
1
Human HCC Cell Line Maintenance
2
Eleven HCC Cell Line Study
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In this study, eleven HCC cell lines were used. MHCC97L and MHCC97H cells were purchased from Cobioer Biosciences Company (Nanjing, China). SMMC-7721, HUH-7, BEL-7404, QSG-7701, PLC/PRF/5, QGY-7701, HEPG2, QGY-7703 and HCCLM3 cells were obtained from Cell Resource Center of Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences (Shanghai, China). All cells were cultured in DMEM medium containing 10% fetal bovine serum (Gibco, Grand Island, NY, USA) in a humidified atmosphere of 5% CO2 at 37°C.
3
Hepatocellular Carcinoma Cell Lines
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In this study, three HCC cell lines (SMMC-7721, SK-Hep1 and HCCLM3) were obtained from Cell Resource Center of Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences (Shanghai, China). Another two HCC cell lines (MHCC97L and MHCC97H) were purchased from Cobioer Biosciences (Nanjing, China). All the cells were cultured in DMEM containing 10% fetal bovine serum (Gibco, Grand Island, NY, USA) in a humidified atmosphere of 5% CO2 at 37 °C.
4
Cultivating Liver Cancer Cell Lines
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Six liver cancer cell lines (HepG2, PLC/PRF/5, MHCC-97H, MHCC-97L, Bel-7402, and SK-Hep-1) were purchased from NANJING COBIOER BIOSCIENCES CO., LTD and cultured in Dulbecco’s Modified Eagle Medium (DMEM) supplemented with 10% fetal bovine serum (FBS) and 1% penicillin-streptomycin. Cells in logarithmic growth phase were applied to experiments.
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