The trypsin-like activity of the dialyzed crude extract was determined with the QAR-AMC substrate. To quantify the activity in the extract, substrate cleavage was compared to that of a trypsin standard curve using known unit (U)/ml concentrations of porcine trypsin (Type IX-S; Sigma, catalog # T0303, 16 000 BAEE U/mg; 1 BAEE unit = change in absorbance at 253 nm of 0.001 per minute at 25°C and pH 7.4 using benzoyl arginine ethyl ester (BAEE) as a substrate). The trypsin standard curve consisted of five concentrations ranging from 20 mU/ml to 300 mU/ml. 50 μl of each concentration of the trypsin standard solutions, as well as cockroach extract diluted 200X in Proteinase Assay Buffer, was loaded in triplicate to the 96-well black plate and 50 μM of the substrate was added for a final concentration of 750 μM as above. Enzyme activity in the cockroach extract was interpolated from the fluorescence (Y-axis) versus U/ml concentration (X-axis) curve generated from the trypsin standard curve.
Type 9 s
Manufactured by Merck Group
The Type IX-S is a piece of laboratory equipment manufactured by Merck Group. It is designed to perform specific functions in a research or testing environment. The core function of the Type IX-S is to [describe core function without extrapolation on intended use]. No further details on the intended use or interpretation of the product's capabilities are provided.
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Lab products found in correlation
2 protocols using type 9 s
1
Quantifying Trypsin-like Activity in Cockroach Extract
2
Trypsin-Mediated Vancomycin Adsorption
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Trypsin (5 mg,
65 000 units, from porcine pancreas, Type IX-S, lyophilized
powder, Sigma-Aldrich) was dissolved in 1 mM HCl solution (50 mL),
and then 1 g of vancomycin-loaded rice granules was added and the
mixture was stirred for 6 h. Afterward, samples were subjected to
SEM analysis immediately.
65 000 units, from porcine pancreas, Type IX-S, lyophilized
powder, Sigma-Aldrich) was dissolved in 1 mM HCl solution (50 mL),
and then 1 g of vancomycin-loaded rice granules was added and the
mixture was stirred for 6 h. Afterward, samples were subjected to
SEM analysis immediately.
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