Ef s 18 200 mm f 3.5 5 6 is lens

The left and right thoracic limbs of each rhesus monkey were subjected to anatomical dissection. As such, the presented results are based on ten thoracic limbs. Photographs, however, were only taken of the left thoracic limb, as the left lateral view is custom in most anatomy books and atlases. A Canon EOS 450D body (Canon Inc., Tokyo, Japan) combined with a Canon EF-S 18–200 mm f/3.5–5.6 IS lens (Canon Inc.) was used. Editing of the photographs was performed using GIMP 2.10.30 (gimp.org) and included cropping, adjusting the lighting, optimizing the color temperature, and providing a plain black background.
In order to facilitate the dissection of the blood vessels, latex injection was performed [16 (link)]. The arterial system of the thoracic limb was filled with red-colored latex (V-sure Eco Latex, Vosschemie Benelux, Belgium) by orthograde injection into the axillary artery. The superficial venous system was filled with blue-colored latex. The cephalic vein was injected in orthograde direction at the level of the carpus (wrist).

The snack bars had a highly heterogeneous colour that could not be analysed directly with a colourimeter, and, for this reason, image analysis and colour elaboration was carried out. An RGB image was collected using a Canon EOS 600D camera equipped a Canon EF-S 18–200mm f/3.5–5.6 IS lens (Canon Inc., Tokyo, Japan) at a 50 cm distance between the lens and the sample. The optimal and control snack bars, placed inside a white plastic box, sized 49 × 51 × 50 cm, with a black base, were photographed from above. The image (2193 × 2683 pixels) was imported into MATLAB R2021a (The MathWorks Inc., Natick, MA, USA) and processed as follows: After masking and background removal, carried out using the HYPER-Tools toolbox (freely available at www.hypertools.org, accessed on 20 April 2023) [33 (link)], the image was converted from RGB colour space to L*a*b* colour space, thus obtaining the respective values of brightness and red and yellow indices per pixel. Then, the absolute frequency distribution and the cumulative frequency distribution of the pixels were calculated per each L*a*b* index to highlight differences in the colour characteristics of the snack bars.