Plant samples were incubated in 2 mL of GUS staining solution (1 mg/mL−1 5-bromo-4-chloro-3-indolyl-b-D-glucuronic acid (X-gluc, Sigma), 1 mM potassium ferricyanide, 0.1% Triton X-100, 0.1 M sodium phosphate buffer [pH 7.0] and 10 mM ethylenediaminetetraacetic acid) in the dark at 37 °C for more than 3 h and then cleared with 75% ethanol to remove chlorophyll. Tissues were photographed under a stereomicroscope (Leica).
5 bromo 4 chloro 3 indolyl b d glucuronic acid x gluc
Manufactured by Merck Group
5-bromo-4-chloro-3-indolyl-b-D-glucuronic acid (X-gluc) is a chromogenic substrate used in molecular biology and biochemistry. It is a compound that undergoes a color change when cleaved by the enzyme beta-glucuronidase, which is commonly used as a reporter gene.
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Lab products found in correlation
2 protocols using 5 bromo 4 chloro 3 indolyl b d glucuronic acid x gluc
1
Histochemical GUS Assay for Plant Samples
2
Histochemical Detection of GUS Activity
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The agroinfiltrated leaves were sacrificed for histochemical detection of GUS activity that was used as a reporter for expression of transgenes. They were incubated with substrate solution (0.08% w/v 5-bromo-4-chloro-3-indolyl-b-Dglucuronic acid (X-Gluc; sigma) in 100 mM Na2HPO4/KH2PO4 pH 7.0, 0.01% w/v Chloramphenicol, 20% Triton X-100, 20% Methanol) at 37 °C for 16 h. The GUS expression was detected in the form of blue precipitates in agroinfiltrated leaves under fluorescent microscope that were produced as a result of enzyme mediated hydrolysis of X-Gluc.
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