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Ab15456

Manufactured by Abcam
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Ab15456 is a primary antibody designed for use in Western blotting, immunohistochemistry, and immunofluorescence applications. This antibody targets a specific protein of interest.

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Lab products found in correlation

Ab11267, by Abcam (3 mentions) Ab68153, by Abcam (3 mentions) Ab7291, by Abcam (3 mentions) Mabs1158 for wb, by Merck Group (3 mentions) Hpa017336, by Merck Group (3 mentions) A10438, by ABclonal (3 mentions) F3165, by Merck Group (3 mentions) Alexa 594, by Thermo Fisher Scientific (2 mentions) Ab201061, by Abcam (2 mentions) 2 nbp2 13075b, by Novus Biologicals (2 mentions) Alexa 488, by Thermo Fisher Scientific (2 mentions) Alexa fluor 488, by Thermo Fisher Scientific (2 mentions) Ab5392, by Abcam (1 mentions) Ab76083, by Abcam (1 mentions) Anti app ct, by Merck Group (1 mentions) Mab5643, by Cell Signaling Technology (1 mentions) Irdye 680 800, by LI COR (1 mentions) Ab9116, by Abcam (1 mentions) Ab18184, by Abcam (1 mentions) F1804, by Merck Group (1 mentions)

4 protocols using ab15456

1

Amyloid Precursor Protein and Presenilins Profiling

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The following primary antibodies were used: anti-APP CT (A8717, Sigma-Aldrich, St. Louis, MO); anti-β-amyloid, 17–24 (clone 4G8) (800701, BioLegend, San Diego, CA); anti-PS1 NT raised against the N terminus of PS1 (APS11) (ab15456, Abcam, Cambridge, MA); anti-PS1 CT raised against the C-terminus of PS1 (mAb5643, Cell Signaling Technology, Danvers, MA); anti-PS1 loop raised against the loop domain between transmembrane domains 6 and 7 of PS1 (E2000Y) (ab76083, Abcam, Cambridge, MA); anti-Syt1 (AB5600, Millipore, Temecula, CA); anti-Gapdh (mAb2118, Cell Signaling Technology, Danvers, MA); anti-MAP2 (ab5392, Abcam, Cambridge, MA); anti-His (ab18184, Abcam, Cambridge, MA); anti-V5 (ab9116, Abcam, Cambridge) and anti-FLAG M2 (F1804, Sigma-Aldrich, St. Louis, MO). Alexa Fluor 488 (ThermoScientific, Waltham, MA) and Cy3-labeled corresponding secondary antibodies (Jackson ImmunoResearch, West Grove, PA) were used for confocal microscopy imaging, and IRDye680/800- (Li-COR, Lincoln, NE) or HRP- (Jackson ImmunoResearch, West Grove, PA) conjugated ones were used for western blotting.
Zoltowska K.M., Maesako M., Lushnikova I., Takeda S., Keller L.J., Skibo G., Hyman B.T, & Berezovska O. (2017). Dynamic presenilin 1 and synaptotagmin 1 interaction modulates exocytosis and amyloid β production. Molecular Neurodegeneration, 12, 15.
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2

Characterization of Protein Targets in Neurodegeneration

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Antibodies against PTPRT (MABS1158 for WB and HPA017336 for IHC) and Flag (F3165) were from Sigma; pSTAT3 (Y705) (AP0070), ADAM10 (A10438) and ADAM17 ( A0821) antibodies were from ABclonal; antibodies against STAT3 (ab68153), a-tubulin (ab7291), β-actin (ab8227), Presenlin-1 (ab15456) and Map2 (ab32454 and ab11267) were obtained from Abcam; antibodies against cleaved-Caspase3 (#9661) , a-tubulin: (#3873S), histon3 (#9715) were purchased from Cell Signaling Tech; antibodies against the 6E10 (SIG-393200) antibody were from Covance. The secondary antibodies used for immunocytochemistry were as follows: chicken anti-mouse or rabbit Alexa 488; donkey anti-mouse or rabbit Alexa 594 (Invitrogen, Eugene, OR); all were used at a dilution of 1:500. ProLong™ Diamond Antifade Mountant with DAPI (Thermo Fisher Scienti c, P36962) was used as a nuclear counterstain at 1 µg/ml. DAPT (γ-secretase inhibitor, D5942), Thio avine S (T1892), Anti-Digoxigenin-AP (Fab fragments, 11093274910), and Phorbol 12-myristate 13-acetate (PMA, p8139) were ordered from Sigma-Aldrich.
Liu S., Zhang Z., Li L., Yao L., Ma Z., & Li J. (2021). ADAM10- and presenilin 1/g-secretase-dependent cleavage of PTPRT mitigates Neurodegeneration of Alzheimer’s disease.
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3

Antibody Panel for Alzheimer's Markers

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Antibodies against PTPRT (MABS1158 for WB and HPA017336 for IHC) and Flag (F3165) were from Sigma; pSTAT3 (Y705) (AP0070), ADAM10 (A10438) and ADAM17 ( A0821) antibodies were from ABclonal; antibodies against A42 (ab201061), STAT3 (ab68153), -tubulin (ab7291), β-actin (ab8227), Presenlin-1 (ab15456), VAMP2 (ab3347) and Map2 (ab32454 and ab11267) were obtained from Abcam; antibodies against cleaved-Caspase3 (#9661) , a-tubulin: (#3873S), histon3 (#9715) were purchased from Cell Signaling Tech; antibody against the A plaque 6E10 (SIG-393200) antibody were from Covance; antibody against oligomeric A MOAB-2 (NBP2-13075) and biotin-labeled MOAB-2 (NBP2-13075B) from Novus Biologicals. The secondary antibodies used for immunocytochemistry were as follows: chicken anti-mouse or rabbit Alexa 488; donkey anti-mouse or rabbit Alexa 594 (Invitrogen, Eugene, OR); all were used at a dilution of 1:500. ProLong™ Diamond Antifade Mountant with DAPI (Thermo Fisher Scientific, P36962) was used as a nuclear counterstain at 1 µg/ml. DAPT (γ-secretase inhibitor, D5942), Thioflavine S (T1892), Anti-Digoxigenin-AP (Fab fragments, 11093274910), and Phorbol 12myristate 13-acetate (PMA, p8139) were ordered from Sigma-Aldrich.
Liu S., Zhang Z., Li L., Yao L., Ma Z., & Li J. (2021). ADAM10- and presenilin 1/γ-secretase-dependent cleavage of PTPRT mitigates neurodegeneration of Alzheimer’s disease.
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4

Antibody-based Characterization of Alzheimer's Pathology

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Antibodies against PTPRT (MABS1158 for WB and HPA017336 for IHC) and Flag (F3165) were from Sigma; pSTAT3 (Y705) (AP0070), ADAM10 (A10438) and ADAM17 ( A0821) antibodies were from ABclonal; antibodies against Ab 42 (ab201061), STAT3 (ab68153), α-tubulin (ab7291), β-actin (ab8227), Presenlin-1 (ab15456), VAMP2 (ab3347) and Map2 (ab32454 and ab11267) were obtained from Abcam; antibodies against cleaved-Caspase3 (#9661), a-tubulin: (#3873S), histon3 (#9715) were purchased from Cell Signaling Tech; antibody against the Ab plaque 6E10 (SIG-393200) antibody were from Covance; antibody against oligomeric Ab MOAB-2 (NBP2-13075) and biotin-labeled MOAB-2 (NBP2-13075B) from Novus Biologicals. The secondary antibodies used for immunocytochemistry were as follows: chicken anti-mouse or rabbit Alexa 488; donkey anti-mouse or rabbit Alexa 594 (Invitrogen, Eugene, OR); all were used at a dilution of 1:500. ProLong™ Diamond Antifade Mountant with DAPI (Thermo Fisher Scienti c, P36962) was used as a nuclear counterstain at 1 µg/ml. DAPT (γ-secretase inhibitor, D5942), Thio avine S (T1892), Anti-Digoxigenin-AP (Fab fragments, 11093274910), and Phorbol 12-myristate 13-acetate (PMA, p8139) were ordered from Sigma-Aldrich.
Liu S., Zhang Z., Li L., Yao L., Ma Z., & Li J. (2021). ADAM10- and Presenilin 1/γ-Secretase-Dependent Cleavage of PTPRT Mitigates Neurodegeneration of Alzheimer’s Disease.
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