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Amx 500

Manufactured by Bruker
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Sourced in United States, Germany

The AMX 500 is a high-performance nuclear magnetic resonance (NMR) spectrometer designed and manufactured by Bruker. It is capable of operating at a magnetic field strength of 500 MHz for proton observation. The AMX 500 is used for the analysis and characterization of a wide range of chemical and biological samples.

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Lab products found in correlation

Jms t100 gcv, by JEOL (5 mentions) Agilent 1260 infinity 2, by Agilent Technologies (5 mentions) Supelcosil lc si, by Merck Group (4 mentions) Agilent 6510 q tof apparatus, by Agilent Technologies (3 mentions) 343 polarimeter, by PerkinElmer (3 mentions) Jnm ex270, by JEOL (3 mentions) Phi 5000 versa probe 2, by Physical Electronics (2 mentions) Ifs 48, by Bruker (2 mentions) Am 400, by Bruker (2 mentions) Jnm ex 270 ft nmr spectrometer, by JEOL (2 mentions) Discovery ascentis rp amide, by Merck Group (2 mentions) Synergi fusion rp, by Phenomenex (2 mentions) Ascentis rp amide, by Merck Group (2 mentions) Synergy mx microplate reader, by Agilent Technologies (2 mentions) Lct premier spectrometer, by Waters Corporation (2 mentions) Avance 3 700, by Bruker (2 mentions) P 2200, by Jasco (1 mentions) S1000 thermal cycler, by Bio-Rad (1 mentions) Drx 500, by Bruker (1 mentions) Peaktrak software, by Teledyne (1 mentions)

47 protocols using amx 500

1

Purification and Characterization of Organic Compounds

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All commercial reagents were used as received. Column chromatography was carried out on 60 N silica gel (Kanto Chemicals). Optical rotations were recorded on JASCO P-2200 digital polarimeter. 1H- and 13C-NMR spectra were recorded on Bruker DRX-500 or Bruker AMX-500 spectrometer (500 MHz for 1H-NMR and 125 MHz for 13C-NMR). NMR spectra were recorded in C6D6 (99.5 atom% enriched, Kanto). 1H chemical shifts were reported in δ value based on residual benzene (7.15 ppm) as a reference. 13C chemical shifts were reported in δ value based on benzene (128.0 ppm) as a reference. Data are reported as follows: chemical shift, multiplicity (s = singlet, d = doublet, t = triplet, q = quartet, m = multiplet, br = broad), coupling constant (Hz), and integration. GC-MS analyses were conducted with MS-2010 (Shimadzu). Mass spectra were obtained with a JEOL JMS-T100GCV (EI mode).
Oligonucleotides for polymerase chain reactions (PCRs) were purchased from Hokkaido System Science Co., Ltd. PCRs were performed with a BioRad S1000 thermal cycler.
Sato H., Narita K., Minami A., Yamazaki M., Wang C., Suemune H., Nagano S., Tomita T., Oikawa H, & Uchiyama M. (2018). Theoretical Study of Sesterfisherol Biosynthesis: Computational Prediction of Key Amino Acid Residue in Terpene Synthase. Scientific Reports, 8, 2473.
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2

NMR Analysis of Purified Compounds

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Reversed-phase C-18 flash chromatography was performed using a Tekedyne Isco Combiflash® RFx4 equipped with PeakTrak software (Version 2.1.19, Teledyne Isco, Lincoln, NE, USA). NMR data for 1 were collected on a JEOL ECA-600 spectrometer (JEOL USA, Peabody, MA, USA) operating at 600.17 MHz for 1H, 150.9 MHz for 13C, and 60.8 MHz for 15N (instrument reference set to liquid NH3). The edited-g-HSQC spectrum was optimized for 140 Hz, the g-HMBC spectrum was optimized for 8 Hz, and the band selective g-HMBC experiment was optimized for 8 Hz. Chemical shifts were referenced to solvent, e.g., DMSO-d6 δH observed at 2.50 ppm and δC observed at 39.51 ppm. Chemical shifts for 15N were referenced to liquid NH3 with long range JH,N optimized for 6 Hz. NMR data for 2 were collected on a Bruker AMX 500 operating at 500 MHz for 1H and 125 MHz for 13C (Bruker Biospin, Billerica, MA, USA). The HRMS spectrum was measured using a JEOL AccuTOF-DART 4G (JEOL USA, Peabody, MA, USA) using a prototype paper spray attachment. Isotope matching was used to confirm the presence of sulfur in the molecule (Figure S2). Calculated NMR spectra were generated using Advanced Chemical Development ChemPredictor Software (Version 11 Advanced Chemical Development, Toronto, ON, Canada).
Wright A.E., Killday K.B., Chakrabarti D., Guzmán E.A., Harmody D., McCarthy P.J., Pitts T., Pomponi S.A., Reed J.K., Roberts B.F., Rodrigues Felix C, & Rohde K.H. (2017). Dragmacidin G, a Bioactive Bis-Indole Alkaloid from a Deep-Water Sponge of the Genus Spongosorites. Marine Drugs, 15(1), 16.
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3

Spectroscopic Characterization of Samples

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FT-IR spectrum was recorded on Bruker IFS 48 (Bruker, Ettlingen, Germany) using Thermo Scientific iD5 Diamond ATR accessory (Thermo Fisher Scientific, Waltham, MA, USA). 1H NMR spectra were measured on Bruker AMX 500 spectrometer (1H, 500.14 MHz) in D2O:DMSO (3:2 v/v) mixture at RT. XPS measurements were carried out using a PHI 5000 Versa Probe II (ULVAC-PHI, Chigasaki, Japan) spectrometer with a monochromatic Al Kα radiation source (E = 1486.6 eV). A dual-beam charge compensation was used to avoid possible charging of samples. High resolution spectra were recorded with the analyzer pass energy set to 46.95 eV. All binding energies were corrected to C–C line at 284.8 eV. Deconvolution of obtained spectra was done with PHI MultiPak software. The spectrum background subtraction was done using the Shirley method.
Lachowicz D., Mielczarek P., Wirecka R., Berent K., Karewicz A., Szuwarzyński M, & Zapotoczny S. (2019). Nanohydrogels Based on Self-Assembly of Cationic Pullulan and Anionic Dextran Derivatives for Efficient Delivery of Piroxicam. Pharmaceutics, 11(12), 622.
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4

Analytical Characterization of Compounds

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Specific rotation, PerkinElmer 343 Polarimeter (PerkinElmer, Waltham, MA, USA); NMR, Bruker AMX 500 (Bruker BioSpin GmbH, Rheinstetten, Germany) (500.12/125.67 MHz (1Н/13C) spectrometer; Bruker AVANCE III-700 spectrometer at 700.13 MHz/176.04 MHz (1H/13C); ESI MS (positive and negative ion modes), Agilent 6510 Q-TOF apparatus (Agilent Technology, Santa Clara, CA, USA), sample concentration 0.01 mg/mL; HPLC, Agilent 1260 Infinity II with a differential refractometer (Agilent Technology, Santa Clara, CA, USA); columns Supelcosil LC-Si (4.6 × 150 mm, 5 µm) and Discovery HS F5-5 (10 × 250 mm, 5 µm) (Supelco, Bellefonte, PA, USA), Diasfer 110 C-8 (4.6 × 250 mm, 5 µm) (Biochemmack, Moscow, Russia).
Silchenko A.S., Avilov S.A., Andrijaschenko P.V., Popov R.S., Chingizova E.A., Grebnev B.B., Rasin A.B, & Kalinin V.I. (2022). The Isolation, Structure Elucidation and Bioactivity Study of Chilensosides A, A1, B, C, and D, Holostane Triterpene Di-, Tri- and Tetrasulfated Pentaosides from the Sea Cucumber Paracaudina chilensis (Caudinidae, Molpadida). Molecules, 27(21), 7655.
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5

NMR Characterization of MTX-Cu(II) Complexes

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1H NMR and 13C NMR measurements were performed on a Bruker AMX-500 instrument (1H: 500 MHz). TSP (trimethylsilyl propanoic acid) was used as an internal standard. Samples were prepared in 500 µl D2O (99.95 %) and the final concentration was 10 mM and 40 mM for proton and carbon spectra, respectively. NMR spectra were recorded for MTX and Cu(II)–MTX system at pD (pH measured by electrode uncorrected for the isotopic effect) value 7.5, which after appropriate correction (Krężel and Bal, 2004 (link)) is equal to 7.4. Measurements were made for solutions at five different Cu(II)–MTX molar ratios 1:500 ÷ 5:500. The pD of samples was adjusted by adding small volumes of concentrated DNO3 or NaOD.
Nagaj J., Kołkowska P., Bykowska A., Komarnicka U.K., Kyzioł A, & Jeżowska-Bojczuk M. (2014). Interaction of methotrexate, an anticancer agent, with copper(II) ions: coordination pattern, DNA-cleaving properties and cytotoxic studies. Medicinal Chemistry Research, 24(1), 115-123.
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6

NMR Analysis of Larval Hemolymph Metabolites

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Hemolymph from 20 larvae of P. cochleariae or C. populi was collected and taken up in 200 μl CD3OD for 1H/2D-exchange. The solution was concentrated under reduced pressure and dissolved in 500 μl CD3OD. One-dimensional 1H NMR spectra were recorded on a Bruker AV400 using water suppression (purge). Two-dimensional double quantum-filtered (dqf)-COSY spectra with phase cycling were recorded on a Bruker AV400. A total of 32 scans were acquired using a time domain of 8 k in F2 (acquisition time of 1.2 s) and 512 increment in F1. Spectra were zero-filled to 8 k × 4 k prior to Fourier transformation and phasing using the Topspin software (Bruker). Heteronuclear HSQC and HMBC spectra were recorded using Bruker AMX500 with a cryoprobe. Samples were dissolved in 100 μl CD3OD using 2 mm NMR vials. For HSQC spectra, 40 scans were acquired using a time domain of 1 k in F2 and 256 increments in F1. For HMBC spectra, 256 scans were acquired using a time domain of 4 k in F2 and 128 increments in F1. Spectra were zero-filled to 4 k × 2 k prior to Fourier transformation and phasing using the Topspin software (Bruker).
Pauls G., Becker T., Rahfeld P., Gretscher R.R., Paetz C., Pasteels J., von Reuss S.H., Burse A, & Boland W. (2016). Two Defensive Lines in Juvenile Leaf Beetles; Esters of 3-nitropropionic Acid in the Hemolymph and Aposematic Warning. Journal of Chemical Ecology, 42, 240-248.
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7

Spectroscopic Analysis of Natural Compounds

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IR spectra were recorded on KBr discs using a Perkin Elmer Spectrum 100 FT-IR-410 spectrometer; ν in cm‒1.1H-, 13C-NMR, DEPT, COSY, HSQC, NOESY, and HMBC spectra: Bruker AMX 500 instrument (at 500 and 125 MHz, respectively); in chloroform-d; δ in ppm rel. to Me4Si as internal standard, J in Hz. HR-ESI-MS APEXIII (Bruker Daltonik) 7 Tesla (ESI-FT-ICR-MS); in m/z. Spectra were processed using the computer software MestreNova 9.1. Column chromatography (CC) was performed over Merck silica gel 60, particle size between 0.043 and 0.063 mm in diameter, and porosity 230–400 mesh ASTM. Evaporation was performed using a BUCHI rotary evaporator under reduced pressure. Plant materials, extracts, and fractions were weighed on a Satorius OT12 electronic mass balance. Pure compounds or fractions were weighed on a Satorius BP221S electronic balance maximum rating 220 g d = 0.1 mg. Analytical TLCs were carried out on pre-coated silica gel 60 F254 aluminum sheets (0.25 mm layer, Merck). The chromatograms were visualized under UV light at 254 and 366 nm and with 10% H2SO4 spray, then heated.
Fotso G.W., Ngameni B., Storr T.E., Ngadjui B.T., Mafu S, & Stephenson G.R. (2020). Synthesis of Novel Stilbene–Coumarin Derivatives and Antifungal Screening of Monotes kerstingii-Specialized Metabolites Against Fusarium oxysporum. Antibiotics, 9(9), 537.
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8

Spectroscopic Characterization of Compounds

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Melting point apparatus Buchi 535 was used to record melting point. Digital Polarimeter; Jasco DIP-360 was used to record optical rotation. Shimadzu UV-240 spectrophotometer was used to record λmax in nm. Infrared spectra (IR) were recorded on Shimadzu IR-460 in nujol or KBr pellets and reported in cm−1. NMR spectra were recorded on Bruker AMX-500 and AM-400 for1 H (300–500 MHz) and13 (link)C (125–150 MHz) spectrometers. "spectra were recorded on Bruker Avance spectrometers ranging from 7.05 up to 14.09 T". Mass spectrometer Jeol-JMS H X-110 was employed to record high resolution electron ionization mass spectra (HR-EIMS).
Javed S., Mahmood Z., Khan K.M., Sarker S.D., Javaid A., Khan I.H, & Shoaib A. (2021). Lupeol acetate as a potent antifungal compound against opportunistic human and phytopathogenic mold Macrophominaphaseolina. Scientific Reports, 11, 8417.
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9

Synthesis of Dendritic Copolymer H40-PLA

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Dendritic copolymer H40-PLA was synthesized by the ring opening polymerization (ROP) of LA with H40 as the macro-initiator and Sn(Oct)2 as the catalyst. LA (4.58 g, 31.77 mmol), H40 (0.50 g, 11.29 mmol of hydroxyl groups), and Sn(Oct)2 (0.013 g, 0.1 mol% of monomer) were added in a glass tube which was connected to a vacuum system. An exhausting-refilling process with argon was then repeated three times. The tube was sealed, heated to 150°C in oil bath for 12 h, and then cooled to room temperature. The crude product was dissolved in tetrahydrofuran (THF) and passed through a neutral alumina column. Then the mixture was concentrated and precipitated in cold diethyl ether to generate white powders H40-PLA. The final product was collected by filtration and dried under vacuum at 40°C for 24 h. 1H NMR (Bruker AMX 500) was used to confirm the structures of dendritic copolymer H40-PLA which dissolved in CDCl3. Molecular weight of the H40-PLA was determined by GPC (Waters GPC analysis system with RI-G1362A refractive index detector, Waters Corp., Milford, MA, USA) with THF as the eluent at the flow rate of 1 mL/min. Molecular weight and polydispersity index were estimated using standard polystyrene samples.
Zhang X., Yang Y., Liang X., Zeng X., Liu Z., Tao W., Xiao X., Chen H., Huang L, & Mei L. (2014). Enhancing Therapeutic Effects of Docetaxel-Loaded Dendritic Copolymer Nanoparticles by Co-Treatment with Autophagy Inhibitor on Breast Cancer. Theranostics, 4(11), 1085-1095.
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10

Characterization of Grafted Polyelectrolytes

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The functional groups were determined using a ThermoFisher Scientific Nicolet iS10 FT-IR spectrometer, equipped with VariGATR grazing angle accessory. Chemical structures were assessed by 1H NMR, recorded on a Bruker AMX-500 instrument. The chemical shifts in 1H NMR spectra are referenced to deuterium hydrogen oxide (semiheavy water, HDO) residual signal as an internal standard. The prepared grafted polyelectrolytes were dissolved in D2O before analysis. FT-IR and 1H NMR spectra of HF-PAAs are shown in Figures S1–S4 in the Electronic Supplementary Information (ESI).
Lamch Ł., Ronka S., Moszyńska I., Warszyński P, & Wilk K.A. (2020). Hydrophobically Functionalized Poly(Acrylic Acid) Comprising the Ester-Type Labile Spacer: Synthesis and Self-Organization in Water. Polymers, 12(5), 1185.
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