Slides were imaged on a Zeiss AxioImager compound microscope or a Leica Sp8/Sp5 confocal microscope as stated. Cells were scored manually in Fiji (ImageJ), or using the Gurdon Institute Imaging Facility's plugin ObjectScan. For cryosections, every epithelial cell along the entire proximal to distal length of a longitudinal section from the center of the trachea was scored. BCs were T1α+ or KRT5+ with the apical surface not reaching the lumen. For cultured cells, at least three random fields of view per insert were scored.
Sp8 sp5 confocal microscope
Manufactured by Leica
The Sp8/Sp5 confocal microscope is a high-performance imaging system designed for advanced microscopy applications. It offers precise control over the excitation and detection of fluorescent signals, allowing for detailed analysis of biological samples. The instrument's core function is to provide a versatile platform for researchers to capture high-resolution, three-dimensional images of their specimens.
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Lab products found in correlation
2 protocols using sp8 sp5 confocal microscope
1
Quantifying Airway Basal Cell Populations
2
Quantifying Epithelial Cell Counts
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Slides were imaged on a Zeiss AxioImager compound, or a Leica Sp8/Sp5 confocal microscope. Cells were scored manually in Fiji. For cryosections, every epithelial cell along the entire proximal to distal length of a longitudinal section from the centre of the trachea was scored. For cultured cells at least three random fields of view from each insert were scored. Raw cell counts are available in Fig. S6 .
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