One NRAS mutant cell line, CRL 1572 (c.35G>A, p. G12D), and two HRAS mutant cell lines, CRL-1671 (c.183G>T, p.Q61H) and CRL-8083 (c.182A>G, p.Q61R), were obtained from ATCC (Manassas, VA). Positive controls for the rest of the mutations were generated by site-directed mutagenesis using the QuikChange® Lightning Site-Directed Mutagenesis Kit (catalog #210518, Stratagene, La Jolla, CA) followed by cloning into plasmids. Leucocytes from a healthy donor were used as a negative control.
Crl 1572
Manufactured by American Type Culture Collection
CRL-1572 is a cell line derived from human embryonic kidney cells. It is a widely used tool for research and development purposes.
Automatically generated - may contain errors
Lab products found in correlation
Fetal bovine serum (fbs), by Thermo Fisher Scientific (2 mentions)
Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (2 mentions)
Crl 1671, by American Type Culture Collection (1 mentions)
Htb 77, by American Type Culture Collection (1 mentions)
Heat inactivated fetal bovine serum, by Thermo Fisher Scientific (1 mentions)
Penicillin, by Merck Group (1 mentions)
Streptomycin, by Merck Group (1 mentions)
Axio vert a1, by Zeiss (1 mentions)
Htb 161, by American Type Culture Collection (1 mentions)
Ccl 75, by American Type Culture Collection (1 mentions)
3 protocols using crl 1572
1
Isogenic Cell Lines for RAS Mutations
2
Culturing Human Ovarian Cancer Cell Lines
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The human OC cell line, PA-1 cells (ATCC® CRL-1572™, Homo sapiens ovary epithelial teratocarcinoma), were cultured with the Eagle’s Minimum Essential medium. The human OC cell line, SKOV3 cells (ATCC® HTB-77™, Homo sapiens ovary epithelial adenocarcinoma) were cultured with the RPMI 1640 medium (Life TechnologiesTM). Both media were supplemented with 10% (v/v) heat-inactivated fetal bovine serum (Gibco®), 2 mM glutamine, 50 U/mL penicillin and 50 mg/mL streptomycin (Sigma-Aldrich®). The cell lines were cultured under a humidified atmosphere of 5% CO2 mixed in 95% air at 37 °C, and they were examined under a Leica DMI 3000B phase contrast microscope (Leica Microsystems, Wetzlar, Germany) regularly. After microscopic examination, these cells were used for the following experiments.
3
Cell Culture Protocols for Cancer Research
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
OC cell lines PA-1 (PA1; (ATCC® CRL-1572™) and NIH:OVCAR-3 (OVCAR3; ATCC® HTB-161™), and a normal lung fibroblast cell line WI-38 (ATCC® CCL-75™) were purchased from ATCC and cultured in Dulbecco's modified Eagle's medium (DMEM; Gibco; Thermo Fisher Scientific, Inc.) supplemented with 10% FBS (Gibco; Thermo Fisher Scientific, Inc.), 100 U/ml penicillin and 100 µg/ml streptomycin at 37°C in a humidified 5% CO2 chamber (HF90; Heal Force Bio-Meditech Holdings Ltd.). Cells were observed under an inverted phase contrat microscope (Zeiss Axiovert. A1; magnification, ×20).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!