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Protein a or protein g coated beads

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Protein A or protein G coated beads are laboratory reagents used for the purification and isolation of antibodies or other proteins that bind to these specific proteins. The beads provide a solid support for the immobilization of protein A or protein G, which can then be used to capture and separate target proteins from complex mixtures. These beads offer a convenient and efficient way to perform affinity-based protein purification.

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Lab products found in correlation

Anti v5 antibody, by Thermo Fisher Scientific (2 mentions) Bas mpphosphor, by Fujifilm (2 mentions) Fla3000 phosphorimager, by Fujifilm (2 mentions) Pngase f, by New England Biolabs (2 mentions)

Close spelling variants of this product

Protein G beads (159 citations) Protein A/G (47 citations) Protein A-coated beads (3 citations)

2 protocols using protein a or protein g coated beads

1

Quantitative Analysis of PD-L1 Turnover

Check if the same lab product or an alternative is used in the 5 most similar protocols
V5-tagged PD-L1 transduced CMTM6 overexpressing A375 cells, and
V5-tagged PD-L1 transduced CMTM6 knockout A375 cells were cultured in
methionine- and cysteine-free medium for 1h at 37°C. Cells were then
pulse labeled with 0.5 mCi/ml [35S]Cys/[35S]Met
(PerkinElmer) for 1 hour. Cells were washed with PBS to remove residual
[35S]Cys/[35S]Met, and then cultured in regular medium
with extra ‘cold’ methionine and cysteine for 0, 1, 2, 3 and 6h.
Cell samples were lysed and used for immunoprecipitation with anti-V5 antibody
(ThermoFisher) immobilized on protein A or protein G coated beads
(ThermoFisher). Immunoprecipitates were either left untreated or treated with
EndoH or PNGaseF (New England Biolabs), according to the manufacturer’s
instructions.
Immunoprecipitates were run on NuPAGE 4-12% gels. Gels were treated with
1M NaSalicylate pH5.6 before drying, and then analysed on Fujifilm BAS-MP
phosphor imager screens. Quantification was performed using a Fujifilm FLA-3000
phosphorimager and AIDA image analyzer software. Gels were exposed to film using
intensifier screens at -80 C.
Mezzadra R., Sun C., Jae L.T., Gomez-Eerland R., de Vries E., Wu W., Logtenberg M.E., Slagter M., Rozeman E.A., Hofland I., Broeks A., Horlings H.M., Wessels L.F., Blank C.U., Xiao Y., Heck A.J., Borst J., Brummelkamp T.R, & Schumacher T.N. (2017). Identification of CMTM6 and CMTM4 as PD-L1 protein regulators. Nature, 549(7670), 106-110.
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2

Quantitative Analysis of PD-L1 Turnover

Check if the same lab product or an alternative is used in the 5 most similar protocols
V5-tagged PD-L1 transduced CMTM6 overexpressing A375 cells, and
V5-tagged PD-L1 transduced CMTM6 knockout A375 cells were cultured in
methionine- and cysteine-free medium for 1h at 37°C. Cells were then
pulse labeled with 0.5 mCi/ml [35S]Cys/[35S]Met
(PerkinElmer) for 1 hour. Cells were washed with PBS to remove residual
[35S]Cys/[35S]Met, and then cultured in regular medium
with extra ‘cold’ methionine and cysteine for 0, 1, 2, 3 and 6h.
Cell samples were lysed and used for immunoprecipitation with anti-V5 antibody
(ThermoFisher) immobilized on protein A or protein G coated beads
(ThermoFisher). Immunoprecipitates were either left untreated or treated with
EndoH or PNGaseF (New England Biolabs), according to the manufacturer’s
instructions.
Immunoprecipitates were run on NuPAGE 4-12% gels. Gels were treated with
1M NaSalicylate pH5.6 before drying, and then analysed on Fujifilm BAS-MP
phosphor imager screens. Quantification was performed using a Fujifilm FLA-3000
phosphorimager and AIDA image analyzer software. Gels were exposed to film using
intensifier screens at -80 C.
Mezzadra R., Sun C., Jae L.T., Gomez-Eerland R., de Vries E., Wu W., Logtenberg M.E., Slagter M., Rozeman E.A., Hofland I., Broeks A., Horlings H.M., Wessels L.F., Blank C.U., Xiao Y., Heck A.J., Borst J., Brummelkamp T.R, & Schumacher T.N. (2017). Identification of CMTM6 and CMTM4 as PD-L1 protein regulators. Nature, 549(7670), 106-110.
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