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Miseq instrument 300 bp kit

Manufactured by Illumina
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The MiSeq Illumina instrument is a sequencing platform that uses a 300 bp kit to perform DNA sequencing. It is designed to generate high-quality, short-read sequencing data.

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Lab products found in correlation

Mo bio power fecal dna isolation kit, by Qiagen (2 mentions) Faster prep fp120, by Thermo Fisher Scientific (2 mentions) Qubit fluromoter, by Thermo Fisher Scientific (2 mentions)

Spelling variants of this product

MiSeq instrument (1030 citations) MiSeq 300 (25 citations) MiSeq 300 bp (11 citations) MiSeq kits (2 citations)

2 protocols using miseq instrument 300 bp kit

1

16S rDNA V4 Amplicon Sequencing

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
A multiplexed amplicon library covering the 16S rDNA gene V4 region was generated from human stool sample DNA as described 58 (link). Briefly, bacterial genomic DNA was extracted using the Mo Bio Power Fecal DNA Isolation kit (Mo Bio Laboratories) according to the manufacturer's instructions. To increase the DNA yields, the following modifications were used. An additional bead beater step using the Faster Prep FP120 (Thermo) at 6 meters/second for 1 min was used instead of vortex agitation. Incubation with buffers C2 and C3 was done for 10 min at 4°C. Starting nucleic acid concentrations were determined by a Qubit Fluromoter (Life Technologies). The amplicon library was prepared using dual-index barcodes 58 (link). The aggregated library pool was size selected from 300-500 base pairs (bp) on a pippin prep 1.5% agarose cassette (Sage Sciences) according to the manufacturer's instructions. The concentration of the pool was measured by qPCR (Kapa Biosystems) and loaded onto the MiSeq Illumina instrument (300 bp kit) at 6-9pM with 40% phiX spike-in to compensate for low base diversity according to Illumina's standard loading protocol.
Abdel-Gadir A., Stephen-Victor E., Gerber G.K., Rivas M.N., Wang S., Harb H., Wang L., Li N., Crestani E., Spielman S., Secor W., Biehl H., Dibendetto N., Dong X., Umetsu D.T., Bry L., Rachid R, & Chatila T.A. (2019). Microbiota Therapy Acts Via a Regulatory T Cell MyD88/RORγt Pathway to Suppress Food Allergy. Nature medicine, 25(7), 1164-1174.
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2

16S rDNA V4 Amplicon Sequencing

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
A multiplexed amplicon library covering the 16S rDNA gene V4 region was generated from human stool sample DNA as described 58 (link). Briefly, bacterial genomic DNA was extracted using the Mo Bio Power Fecal DNA Isolation kit (Mo Bio Laboratories) according to the manufacturer's instructions. To increase the DNA yields, the following modifications were used. An additional bead beater step using the Faster Prep FP120 (Thermo) at 6 meters/second for 1 min was used instead of vortex agitation. Incubation with buffers C2 and C3 was done for 10 min at 4°C. Starting nucleic acid concentrations were determined by a Qubit Fluromoter (Life Technologies). The amplicon library was prepared using dual-index barcodes 58 (link). The aggregated library pool was size selected from 300-500 base pairs (bp) on a pippin prep 1.5% agarose cassette (Sage Sciences) according to the manufacturer's instructions. The concentration of the pool was measured by qPCR (Kapa Biosystems) and loaded onto the MiSeq Illumina instrument (300 bp kit) at 6-9pM with 40% phiX spike-in to compensate for low base diversity according to Illumina's standard loading protocol.
Abdel-Gadir A., Stephen-Victor E., Gerber G.K., Rivas M.N., Wang S., Harb H., Wang L., Li N., Crestani E., Spielman S., Secor W., Biehl H., Dibendetto N., Dong X., Umetsu D.T., Bry L., Rachid R, & Chatila T.A. (2019). Microbiota Therapy Acts Via a Regulatory T Cell MyD88/RORγt Pathway to Suppress Food Allergy. Nature medicine, 25(7), 1164-1174.
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