Rabbit anti ferritin

Manufactured by Abcam

Sourced in China

Rabbit anti-ferritin is a primary antibody that binds to the ferritin protein. Ferritin is a ubiquitous intracellular protein involved in the storage of iron. This antibody can be used in various immunochemical techniques to detect and quantify ferritin levels in biological samples.

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Lab products found in correlation

Goat anti iba1, by Abcam (2 mentions) Mouse anti th, by Immunostar (1 mentions) Alexa fluor 488 donkey anti rabbit igg h l, by Thermo Fisher Scientific (1 mentions) Alexa fluor 594 donkey anti goat igg, by Thermo Fisher Scientific (1 mentions) Mouse anti cd68, by Abcam (1 mentions) 4 6 diamidino 2 phenylindole (dapi), by Merck Group (1 mentions) Horse anti mouse, by Vector Laboratories (1 mentions) Ripa lysis buffer, by Beyotime (1 mentions) Rabbit anti p62, by Cell Signaling Technology (1 mentions) Secondary anti rabbit hrp igg antibodies, by Cell Signaling Technology (1 mentions) Rabbit anti lc3, by Proteintech (1 mentions) Rabbit anti p21 waf1 cip1, by Cell Signaling Technology (1 mentions) Rabbit anti p16 ink4a, by Proteintech (1 mentions)

Spelling variants of this product

Ferritin (27 citations) Anti-ferritin (16 citations) Rabbit anti- (5 citations) Rabbit anti-Ferritin monoclonal antibody (2 citations)

3 protocols using rabbit anti ferritin

Immunohistochemical Analysis of Iron Regulatory Proteins

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The brain sections were incubated with normal serum from the animal species that produced the secondary antibodies for 30 min and then incubated with mouse anti-transferrin (TF; Santa Cruz, 1:1000), mouse anti-TF receptor 1 (TFR1; Thermo, 1:500), rabbit anti-TF receptor 2 (TFR2; Abcam, 1:200), rabbit anti-ferroportin (FPn; Thermo, 1:1000), rabbit anti-ferritin (Abcam, 1:500), goat anti-Iba1 (Abcam, 1:200), and mouse anti-TH (ImmunoStar, 1:2000) overnight at 4 °C. Subsequently, the sections were incubated with DyLight 594-labeled goat anti-mouse IgG or goat anti-rabbit IgG. Finally, the sections were labeled with 4′ 6-diamidino-2-phenylindole (DAPI) (H-1500, Vector Labs, USA), mounted with anti-fluorescence quenching mounting medium, and observed under a confocal laser scanning microscope (SP8, Leica, Germany).

Guo J.J., Yue F., Song D.Y., Bousset L., Liang X., Tang J., Yuan L., Li W., Melki R., Tang Y., Chan P., Guo C, & Li J.Y. (2021). Intranasal administration of α-synuclein preformed fibrils triggers microglial iron deposition in the substantia nigra of Macaca fascicularis. Cell Death & Disease, 12(1), 81.

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Immunohistochemistry and Immunofluorescence Staining Protocols

Cited 1 time

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The protocols of immunohistochemistry and immunofluorescence staining have been described previously(Xi et al., 1999 (link), Wan et al., 2020 (link)). The primary antibodies were rabbit anti-heme oxygenase-1 (HO-1, 1:400 dilution; Enzo, New York, NY), goat anti-Iba1 (1:400 dilution; Abcam, Cambridge, MI), mouse anti-CD68 (1:200 dilution; Abcam), rabbit anti-ferritin (1:200 dilution; Abcam). Negative controls were conducted by omitting the primary antibody. Secondary antibodies were horse anti-mouse (1:500 dilution, Vector Laboratories Inc, Burlingame, CA), goat anti-rabbit (1:500 dilution, Invitrogen, Eugene, OR), rabbit anti-goat (1:500 dilution, Invitrogen), Alexa Fluor 594 donkey anti-goat IgG (1:500 dilution; Invitrogen), Alexa Fluor 488 donkey anti-rabbit IgG (H+L) (1:500 dilution; Invitrogen). Nuclei were stained with 4′, 6-diamidino-2-phenylindole (DAPI) (Sigma-Aldrich, St. Louis, MO) in immunofluorescence studies and hematoxylin for immunohistochemistry.

Ye F., Hua Y., Keep R.F., Xi G, & Garton H.J. (2021). CD47 Blocking Antibody Accelerates Hematoma Clearance and Alleviates Hydrocephalus After Experimental Intraventricular Hemorrhage. Neurobiology of disease, 155, 105384.

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Western Blot Protocol for Protein Analysis

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Cells were lysed in RIPA lysis buffer (Cat# P0013K, Beyotime, China) added with Protease inhibitors cocktail. Equal volumes of proteins were separated by 12 % sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and electro-transferred onto a polyvinylidence difluoride (PVDF) membrane. Primary rabbit anti-FECH (Cloud-Clone, Wuhan, China), rabbit anti-Ferritin (Abcam, Cambridge, UK), rabbit anti-GPX4 (HuaBio, Hangzhou, China), rabbit anti-p21-Waf1/Cip1 (Cell Signaling Technology, Danvers, USA), rabbit anti-p16-INK4A (Proteintech, Chicago, USA), rabbit anti-LC3 (Proteintech, Chicago, USA), rabbit anti-p62 (Cell Signaling Technology, Danvers, USA), rabbit anti-actin (Cloud-Clone, Wuhan, China), and secondary anti-rabbit HRP-IgG antibodies (Cell Signaling Technology, Danvers, USA) were used for immunoblotting.

Wang T., Yang C., Li Z., Li T., Zhang R., Zhao Y., Cheng T., Zong Z., Ma Y., Zhang D, & Deng H. (2023). Flavonoid 4,4′-dimethoxychalcone selectively eliminates senescent cells via activating ferritinophagy. Redox Biology, 69, 103017.

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