Log In Sign up
The largest database of trusted experimental protocols

Mouse ige ready set go

Manufactured by Thermo Fisher Scientific
Visit Supplier

The Mouse IgE Ready-Set-Go is a quantitative ELISA kit designed to measure mouse immunoglobulin E (IgE) levels in biological samples.

Automatically generated - may contain errors

Lab products found in correlation

Mouse il 17 duoset elisa, by R&D Systems (1 mentions) Mouse il 23 duoset elisa, by R&D Systems (1 mentions)

Close spelling variants of this product

Ready-SET-Go (98 citations) Mouse IgE ELISA Ready-SET-Go kit (6 citations) Mouse IgE ELISA Ready-SET-Go (3 citations)

4 protocols using mouse ige ready set go

1

Quantification of Mouse Serum IgE

Check if the same lab product or an alternative is used in the 5 most similar protocols
From mice included in the phenotyping study, blood samples were collected via cardiac puncture. Sera were separated by centrifugation and frozen at –20 °C for subsequent analysis of total IgE. A standard colorimetric sandwich ELISA was performed according to manufacturer directions (Mouse IgE Ready-Set-Go!, eBioscience, San Diego, CA). The lower limit of sensitivity was 4 ng/ml.
Shane H.L., Lukomska E., Stefaniak A.B, & Anderson S.E. (2017). Divergent hypersensitivity responses following topical application of the quaternary ammonium compound, didecyldimethylammonium bromide. Journal of immunotoxicology, 14(1), 204-214.
+ Open protocol
+ Expand
2

Quantification of Mouse Serum IgE

Check if the same lab product or an alternative is used in the 5 most similar protocols
From mice included in the phenotyping study, blood samples were collected via cardiac puncture. Sera were separated by centrifugation and frozen at –20 °C for subsequent analysis of total IgE. A standard colorimetric sandwich ELISA was performed according to manufacturer directions (Mouse IgE Ready-Set-Go!, eBioscience, San Diego, CA). The lower limit of sensitivity was 4 ng/ml.
Shane H.L., Lukomska E., Stefaniak A.B, & Anderson S.E. (2017). Divergent hypersensitivity responses following topical application of the quaternary ammonium compound, didecyldimethylammonium bromide. Journal of immunotoxicology, 14(1), 204-214.
+ Open protocol
+ Expand
3

Evaluation of Serum IgE, IL-23, and IL-17 Levels

Check if the same lab product or an alternative is used in the 5 most similar protocols
The levels of serum-IgE were evaluated by using Mouse IgE Ready-Set-Go (eBioscience). The detection limit was 4 ng/ml and results were shown as ng/ml IgE. Concentration of IL-23 in LHG was measured with Mouse IL-23 DuoSet ELISA (R&D Systems). The limit of detection was 4 ng/ml and results were shown as ng/ml IL-23. Concentration of IL-17 in LHG was measured with Mouse IL-17 DuoSet ELISA (R&D Systems). The limit of detection was 4 ng/ml and results were shown as ng/ml IL-17.
Leitner M., Heck S., Nguyen K., Nguyen P.Q., Harfoush S., Rosenkranz E., Bals R, & Dinh Q.T. (2022). Allergic airway inflammation induces upregulation of the expression of IL-23R by macrophages and not in CD3 + T cells and CD11c+F4/80− dendritic cells of the lung. Cell and Tissue Research, 389(1), 85-98.
+ Open protocol
+ Expand
4

Evaluating IgE Response to DDAC

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
To further evaluate type of hypersensitivity response induced by DDAC, IgE was evaluated following dermal exposure. Since we previously identified that exposure duration may influence the development of allergic responses (Shane et al., 2017 (link)), IgE levels in mice exposed to DDAC for 14-days were assessed at 28 days post initial exposure. Mice (n=5) were treated for 14-days with DDAC and rested for 14-days. Blood samples were collected via cardiac puncture and sera was then separated by centrifugation and frozen at −20°C for subsequent analysis. To quantify serum IgE levels a standard colorimetric sandwich ELISA was performed according to manufacturer directions (Mouse IgE Ready-Set-Go!, eBioscience). To determine local production of IgE the IgE+B220+ (IgE+ B-cells) population were analyzed by flow cytometry as previously described using IgE-FITC (R35–72) (BD Biosciences) (Shane et al., 2017 (link)).
Shane H.L., Lukomska E, & Anderson S.E. (2019). Topical application of the quaternary ammonium compound didecyldimethylammonium chloride activates type 2 innate lymphoid cells and initiates a mixed-type allergic response. Toxicological sciences : an official journal of the Society of Toxicology, 168(2), 508-518.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!