Absolute methanol

Manufactured by Carlo Erba

Sourced in France

Absolute methanol is a high-purity, anhydrous form of methanol. It is a clear, colorless, and volatile liquid. The core function of absolute methanol is to serve as a solvent and reagent in various laboratory applications.

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Lab products found in correlation

Pentylenetetrazole, by Merck Group (2 mentions) Chloroform, by Loba Chemie (2 mentions) Ferric chloride, by Avantor (1 mentions) Glacial acetic acid, by Avantor (1 mentions) Hydrochloric acid (hcl), by Avantor (1 mentions) Sulfuric acid, by Avantor (1 mentions) Ultrospec 4000, by GE Healthcare (1 mentions) Sulphuric acid, by HiMedia (1 mentions) Ferric chloride, by BD (1 mentions) Lead acetate test, by Thermo Fisher Scientific (1 mentions) Aluminum nitrate nonahydrate, by Merck Group (1 mentions) Trimethyl trimesate, by Merck Group (1 mentions) Absolute ethanol, by Carlo Erba (1 mentions) Milli q system, by Merck Group (1 mentions)

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Methanol (126 citations)

7 protocols using absolute methanol

Castor Oil Extraction and Analysis

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All solvents used for the extraction process are of laboratory grade. Drugs and chemicals used in the study include: castor oil (Amman Pharmaceutical Industries, Jordan), activated charcoal (Acuro Organics Ltd, New Delhi, India), Loperamide (Daehwa Pharmaceuticals, Republic of Korea), distilled water (Ethiopian Pharmaceutical Manufacturing Factory, Epharm, Ethiopia), Tweens 80 (Atlas Chemical Industries Inc, India), chloroform (Hi-Media Laboratory Reagents, India), absolute methanol (Carlo Erba reagents, S.A.S., France), glacial acetic acid (BDH Laboratory Supplies Poole, England), sulfuric acid (BDH Laboratory Supplies Poole, England), ammonia(BDH Limited poole, England), hydrochloric acid(BDH Laboratory Supplies Poole, England), acetic anhydride (May and Baker LTD Dagenham, England), ferric chloride (BDH Laboratory Supplies Poole, England), Mayer’s and Dragendorff’s reagents(May and Baker LTD Dagenham, England).

Sisay M., Engidawork E, & Shibeshi W. (2017). Evaluation of the antidiarrheal activity of the leaf extracts of Myrtus communis Linn (Myrtaceae) in mice model. BMC Complementary and Alternative Medicine, 17, 103.

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NBT Assay for Intracellular ROS Detection

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The reduction of the NBT salt to diformazan crystals is considered as an indirect measurement of the intracellular ROS production [36 (link)]. PBLs (5 × 10⁵) were incubated with 335-μg/mL NBT (w/v in supplemented RPMI 1640) for 2 h at 37 °C and 5% CO₂, then rinsed three times with absolute methanol (Carlo Erba). The absorbances of diformazan crystals, firstly dissolved with a freshly prepared 2-M KOH/DMSO solution, were read by using an Ultrospec 4000 UV/visible spectrophotometer (Pharmacia Biotech, Stockholm, Sweden) set at 630 nm.

Vergallo C., Panzarini E., Tenuzzo B.A., Mariano S., Tata A.M, & Dini L. (2020). Moderate Static Magnetic Field (6 mT)-Induced Lipid Rafts Rearrangement Increases Silver NPs Uptake in Human Lymphocytes. Molecules, 25(6), 1398.

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Phytochemical Analysis of Castor Oil

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Absolute methanol (Carlo Erba reagents, S.A.S, France), castor oil (Amman Pharmaceutical Industries, Jordan), activated charcoal (Acuro Organics Ltd, New Delhi, India), loperamide (Daehwa Pharmaceutical, Republic of Korea), distilled water (Ethiopian Pharmaceutical Manufacturing Factory, Epharm, Ethiopia), Wagner's reagent (Research-Lab Fine Chem Industries, India), lead acetate 1% (Guangdong Guanghua chemicals factories, China), sodium hydroxide (Blulux Laboratories (P) Ltd., India), ferric chloride (Blulux Laboratories (P) Ltd., India), chloroform (Hi-Media Laboratory Reagents, India), sulphuric acid (HiMedia Laboratories Pvt. Ltd., India), and lyophilizer (Lab freeze Instruments Group Co., Ltd., Japan) were used. All experimental supplies were analytical grade and purchased from Gondar Pharmaceutical Fund Supply Agency and nearby suppliers.

Ferede Y.A., Zewdu W.S., Zeleke M.M, & Alemu M.A. (2021). Evaluation of Antidiarrheal Activity of 80% Methanolic Extract of the Leaves of Cordia africana (Lamiaceae) in Mice. Evidence-based Complementary and Alternative Medicine : eCAM, 2021, 3627878.

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Neuropharmacological Evaluation of Chemicals

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Among the chemicals and solvents used in this study were distilled water (Ethiopian Pharmaceutical Manufacturing, Ethiopia), absolute methanol (Carlo Erba, France), chloroform (Loba Chemie, India), Phenytoin (Brawn, India), Sodium Valproate (Elfin Drugs, India), n-butanol (Indenta, India), Normal saline (Aculife Health Care, India) and Pentylenetetrazole (Sigma-Aldrich Chemie, USA). All of the chemicals and drugs utilized in this study were analytical grade.

Fisseha N., Hammeso W.W, & Nureye D. (2022). Anticonvulsant Activity of Hydro Alcoholic Extract and Solvent Fractions of Biophytum umbraculum Welw. Syn (Oxalidaceae) Root in Mice. Journal of Experimental Pharmacology, 14, 291-299.

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Anticonvulsant Medication Evaluation

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Absolute methanol (Carlo Erba, France), chloroform (Loba Chemie, India), phenytoin (Brawn, India), sodium valproate (Elfin Drugs, India), n-butanol (Indenta, India), normal saline (Aculife Healthcare, India), pentylenetetrazole (Sigma-Aldrich Chemie, USA), distilled water (Ethiopian Pharmaceutical Manufacturing, Ethiopia), and electroconvulsiometer (Rolex, India).

Fisseha N., Shibeshi W, & Bisrat D. (2021). Evaluation of Anticonvulsant Activity of 80% Methanolic Root Bark Extract and Solvent Fractions of Pentas schimperiana (A. Rich.) Vatke (Rubiaceae) in Swiss Albino Mice. Advances in Pharmacological and Pharmaceutical Sciences, 2021, 6689879.

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Phytochemical Profiling of Medicinal Plants

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To conduct this experiment, the following drugs and chemicals were used: distilled water, loperamide hydrochloride (Remedica, Cyprus), castor oil (Amman Pharmaceutical Industries, Jordan), absolute methanol (Carlo Erba Reagents, SAS, France), chloroform (Hi-Media Laboratory Reagents, India), n-butanol (Blulux Laboratories Ltd., India), activated charcoal (Acurouate Organics Ltd., New Delhi), Mayer's reagent and ethyl acetate (May and Baker Ltd., Dagenham, England), ferric chloride (BDH Ltd., England), potassium ferrocyanide (BDH Ltd., England), ammonia (Merck Millipore, India), acetic anhydride (Techno Pharmchem, India), lead acetate test (Fisher Scientific, UK), and sulfuric acid (Farmitalia Carlo Erba, Italy).

Tessema Desta G., Andargie Ferede Y., Sisay Zewdu W, & Adela Alemu M. (2022). Evaluation of Antidiarrheal Activity of 80% Methanol Extract and Solvent Fractions of the Leaves of Withania somnifera (L.) Dunal in Swiss Albino Mice. Evidence-based Complementary and Alternative Medicine : eCAM, 2022, 7968973.

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Synthesis of nanoMOF-based lipid vesicles

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Materials 1,3,5-benzenetricarboxylic acid (trimesate, 95%, Sigma-Aldrich, France) aluminum nitrate nonahydrate (98%, Sigma-Aldrich, France), trimethyl trimesate (98%, Sigma-Aldrich, Saint-Quentin-Fallavier, France) and absolute methanol (99%, Carlo Erba, France) were used for the synthesis of nanoMOFs. FO and FP were purchased from Sigma-Aldrich (France) and Avanti Polar Lipids (France), respectively. Absolute ethanol (99%, Carlo Erba, France) was used for FP@nanoMIL-100. Water was purified by a Millipore MilliQ system.

Porcino M., Li X., Gref R, & Martineau-Corcos C. (2021). Solid-state NMR spectroscopy as a powerful tool to investigate the location of fluorinated lipids in highly porous hybrid organic-inorganic nanoparticles. Magnetic resonance in chemistry : MRC, 59(9-10).

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