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Cholesterol chol

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The Cholesterol (Chol) lab equipment is designed to measure the concentration of cholesterol in a given sample. It provides accurate and reliable data for medical professionals to assess an individual's cholesterol levels. The core function of this product is to facilitate the analysis of cholesterol in a laboratory setting.

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Lab products found in correlation

1 2 dioleoyl 3 trimethylammonium propane methyl sulfate salt dotap, by Avanti Polar Lipids (2 mentions) Poly l glutamic acid sodium salt, by Merck Group (1 mentions) Hepes koh, by Fujifilm (1 mentions) Amiloride hydrochloride hydrate, by Merck Group (1 mentions) Filipin complex, by Merck Group (1 mentions) Sucrose, by Fujifilm (1 mentions) Chondroitin sulfate c sodium salt, by Fujifilm (1 mentions) 1 1 dioctadecyl 3 3 3 3 tetramethylindocarbocyanine perchlorate dii, by Thermo Fisher Scientific (1 mentions) 1 1 dioctadecyl 3 3 3 3 tetramethylindodicarbocyanine perchlorate, by Thermo Fisher Scientific (1 mentions) Hoechst 33342, by AnaSpec (1 mentions) Drabkin s reagent, by Merck Group (1 mentions) Stigmasterol, by Tokyo Chemical Industry (1 mentions) Dotap, by Avanti Polar Lipids (1 mentions) Dnase 1, by Takara Bio (1 mentions) Kod plus dna polymerase, by Toyobo (1 mentions) Ecori, by Promega (1 mentions) 1 palmitoyl 2 oleoyl sn glycero 3 phosphate, by Avanti Polar Lipids (1 mentions) 1 2 distearoyl sn glycero 3 phosphate, by Avanti Polar Lipids (1 mentions) 1 2 dioleoyl sn glycero 3 phosphate, by Avanti Polar Lipids (1 mentions) 1 2 dipalmitoyl sn glycero 3 phosphoserine, by Avanti Polar Lipids (1 mentions)

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Cholesterol (50 citations)

18 protocols using cholesterol chol

1

Lipid-Based Nanoparticle Formulation

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Hydrogenated soy phosphatidylcholine (HSPC) and 1,2‐distearoyl‐sn‐glycero‐3‐phosphoethanolamine‐n‐(methoxy [polyethylene glycol]‐2000) (mPEG2000‐DSPE) were generously donated by NOF (Tokyo, Japan). Cholesterol (Chol) was purchased from Wako Pure Chemical (Osaka, Japan). Oxaliplatin (l‐OHP) was generously donated by Taiho Pharmaceutical (Tokyo, Japan). All lipids were used without further purification. All other reagents were of analytical grade.
Shimizu T., Abu Lila A.S., Nishio M., Doi Y., Ando H., Ukawa M., Ishima Y, & Ishida T. (2017). Modulation of antitumor immunity contributes to the enhanced therapeutic efficacy of liposomal oxaliplatin in mouse model. Cancer Science, 108(9), 1864-1869.
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2

Lipid-Polymer Nanoparticle Synthesis

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1,2-Dioleoyl-3-trimethylammonium-propane methyl sulfate salt (DOTAP) was obtained from Avanti Polar Lipids Inc. (Alabaster, AL, USA). Poly-l-glutamic acid sodium salt (PGA, 10.5?kDa) was purchased from Sigma-Aldrich Co. (St. Louis, MO, USA). Poly-(a,ß)-dl-aspartic acid (PAA, 21?kDa) was obtained from the PolySciTech division of Akina, Inc. (West Lafayette, IN, USA). Cholesterol (Chol) and chondroitin sulfate C sodium salt (CS) were purchased from Wako Pure Chemical Industries, Ltd. (Osaka, Japan). All other chemicals were of the finest grade available.
Hattori Y., Nakamura A., Arai S., Nishigaki M., Ohkura H., Kawano K., Maitani Y, & Yonemochi E. (2014). In vivo siRNA delivery system for targeting to the liver by poly-l-glutamic acid-coated lipoplex. Results in Pharma Sciences, 4, 1-7.
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3

Lipid Vesicle Preparation Protocol

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L-α-phosphatidylcholine (PC) (chicken egg) and L-α-phosphatidylethanolamine (PE) (chicken egg) were purchased from Avanti Polar Lipids (Alabaster, USA) and a dye-labelled lipid β-BODIPY® 530/550 C5-HPC (BODIPY-PC) was purchased from Invitrogen (currently Thermo Fisher Scientific, Eugene, USA). They were used without further purification. Cholesterol (Chol) and the chemicals for a buffer solution (120 mM KCl and 10 mM HEPES/KOH [pH 7.2]) were purchased from Wako Pure Chemicals Industries (Osaka, Japan). Chol was recrystallized thrice from methanol.
Tero R., Fukumoto K., Motegi T., Yoshida M., Niwano M, & Hirano-Iwata A. (2017). Formation of Cell Membrane Component Domains in Artificial Lipid Bilayer. Scientific Reports, 7, 17905.
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4

Isolation and characterization of extracellular vesicles

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HSPC, DOPE and 1,2-dioleoyl-3-trimethylammonium-propane, chloride salt (DOTAP) were generously donated by NOF (Tokyo, Japan). Cholesterol (CHOL) and sucrose were purchased from Wako Pure Chemical (Osaka, Japan). O,O′-ditetradecanoyl-N-(alpha-trimethyl ammonio acetyl) diethanolamine chloride (DC-6–14) was purchased from Sogo Pharmaceutical (Tokyo, Japan). Cytochalasin D,CPZ, amiloride hydrochloride hydrate and filipin complex were purchased from Sigma Aldrich (MO, US). All Abs were purchased from Abcam (Cambridge, UK), including anti-CD9 (RabMab, ab92726), anti-annexin-A2 (ab41803), anti-flotillin-1 (ab41927), anti-EGF (ab9695), anti-TSG101 (ab30871) and HRP (horseradish peroxidase) conjugated goat anti-rabbit IgG (immunoglobulin G) H&L (ab6721). Exosome-depleted (EV-depleted) fetal bovine serum (FBS) was purchased from System Biosciences (CA, US). All other reagents were of analytical grade.
Emam S.E., Ando H., Lila A.S., Shimizu T., Okuhira K., Ishima Y., Mahdy M.A., Ghazy F.E., Sagawa I, & Ishida T. (2018). Liposome co-incubation with cancer cells secreted exosomes (extracellular vesicles) with different proteins expressions and different uptake pathways. Scientific Reports, 8, 14493.
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5

Synthesis and Characterization of DOTAP Lipoplexes

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1,2-Dioleoyl-3-trimethylammonium-propane (DOTAP) methyl sulfate salt was obtained from Avanti Polar Lipids Inc., (Alabaster, AL, USA). Cholesterol (Chol) and chondroitin sulfate C sodium salt were purchased from Wako Pure Chemical Industries, Ltd. (Osaka, Japan). All other chemicals were of the finest grade available.
Hattori Y., Kikuchi T., Ozaki K.I, & Onishi H. (2017). Evaluation of in vitro and in vivo therapeutic antitumor efficacy of transduction of polo-like kinase 1 and heat shock transcription factor 1 small interfering RNA. Experimental and Therapeutic Medicine, 14(5), 4300-4306.
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6

Lipid-Based Nanoparticle Formulation Protocol

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Hydrogenated soy phosphatidylcholine (HSPC) and 1,2-distearoyl-sn-glycero-3-phosphoethanolamine-n-[methoxy(polyethylene glycol)-2000] (mPEG2000-DSPE) were generously donated by NOF (Tokyo, Japan). Cholesterol (CHOL) was purchased from Wako Pure Chemical (Osaka, Japan). S-1, an oral fluorouracil antitumor drug that contains three pharmacological agents (tegafur, 5-chloro-2, 4-dihydroxypyridine, and potassium oxonate) was generously donated by Taiho Pharmaceutical (Tokyo, Japan). 1,1′-Dioctadecyl-3,3,3′,3′-tetramethylindocarbocyanine perchlorate (DiI) and 1,1′-dioctadecyl-3,3,3′,3′-tetramethylindodicarbocyanine perchlorate (DiD) were purchased from Invitrogen (Paisley, UK). Fluorescein isothiocyanate (FITC)-labeled rabbit anti-rat CD31 IgG heavy and light chain polyclonal antibody was purchased from Abcam (Cambridge, UK). Hoechst 33342 and 3,3′- diheptyloxacarbocyanine iodide (DiOC7) were purchased from AnaSpec (Freemont, CA, USA). FITC-dextran (M.W. 150,000) and Drabkin’s reagent was purchased from Sigma-Aldrich (St Louis, MO, USA). In Situ Cell Death Detection Kit (TMR red) was purchased from Roche Diagnostics (Indianapolis, IN, USA). All other reagents were of analytical grade.
Doi Y., Abu Lila A.S., Matsumoto H., Okada T., Shimizu T, & Ishida T. (2016). Improvement of intratumor microdistribution of PEGylated liposome via tumor priming by metronomic S-1 dosing. International Journal of Nanomedicine, 11, 5573-5582.
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7

Lipid-Based Nanoparticle Formulations

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Phosphatidylcholine (PC) from soybean (90%) was generously supplied by Lipoid GmbH (Ludwigshafen, Germany). Cholesterol (Chol) was purchased from Wako Pure Chemical Industries (Osaka, Japan). Butylpyridinium chloride, laurylpyridinium chloride, and cetylpyridinium chloride (CPC) were purchased from MP Biomedicals (Santa Ana, California, USA). Sodium hexadecyl sulfate (SHS) was purchased from Tokyo Chemical Industry Co., Ltd (Tokyo, Japan). MX was supplied by Sigma-Aldrich Production GmbH, (Buchs, Switzerland). All other chemicals used were of reagent grade and were purchased from Wako Pure Chemical Industries.
Duangjit S., Pamornpathomkul B., Opanasopit P., Rojanarata T., Obata Y., Takayama K, & Ngawhirunpat T. (2014). Role of the charge, carbon chain length, and content of surfactant on the skin penetration of meloxicam-loaded liposomes. International Journal of Nanomedicine, 9, 2005-2017.
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8

Lipid Composition Analysis for Formulations

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We obtained the following from the respective suppliers: 1,2-dioleoyl-3-trimethylammonium-propane methyl sulfate salt (DOTAP; Avanti Polar Lipids Inc.), dimethyldioctadecylammonium bromide (DDAB, product name: DC-1-18; Sogo Pharmaceutical Co., Ltd.), cholesterol (Chol; Wako Pure Chemical Industries, Ltd.), β-sitosterol (Sito; Tama Biochemical Co., Ltd.), Ergosterol (Ergo) and stigmasterol (Stig; Tokyo Chemical Industry Co., Ltd.). All other chemicals were of the highest available grade.
Hattori Y., Saito H., Oku T, & Ozaki K.I. (2021). Effects of sterol derivatives in cationic liposomes on biodistribution and gene-knockdown in the lungs of mice systemically injected with siRNA lipoplexes. Molecular Medicine Reports, 24(2), 598.
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9

Synthesis and Characterization of Lipid Molecules

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1-Palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC) was purchased from NOF (Tokyo, Japan), and 1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N-[methoxy(polyethyleneglycol)-1000] (DSPE-1K) was purchased from Nanocs, Inc. (New York, NY, USA). Cholesterol (chol) was purchased from Wako Pure Chemical Industries (Osaka, Japan). Membrane lipid molecule V, lipophilic catalyst C, catalyst C tagged with BODIPY (BODIPY-C), and membrane lipid precursor V* were synthesized, as previously reported [26 (link),27 (link)]. The restriction enzyme EcoRI, pBR322 vector Wizard® SV Gel, and PCR Clean-up system for purification were purchased from Promega Corporation (Madison, WI, USA). The primers were purchased from Sigma-Aldrich (St. Louis, MO, USA). A primer tagged with Texas Red (TR-primer) was purchased from Fasmac (Kanagawa, Japan). DNA ladder markers and DNase I were purchased from Takara Bio (Shiga, Japan). KOD-plus-DNA polymerase was purchased from Toyobo (Osaka, Japan). 2-(4,4-Difluoro-5,7-dimethyl-4-bora-3a,4a-diaza-s-indacene-3-dodecanoyl)-1-hexadecanoyl-sn-glycero-3-phosphocholine (BODIPY-HPC) was purchased from Thermo Fisher Scientific (Waltham, MA, USA).
Hirata Y., Matsuo M., Kurihara K., Suzuki K., Nonaka S, & Sugawara T. (2021). Colocalization Analysis of Lipo-Deoxyribozyme Consisting of DNA and Protic Catalysts in a Vesicle-Based Protocellular Membrane Investigated by Confocal Microscopy. Life, 11(12), 1364.
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10

Synthesis of DOTAP Lipid Nanoparticles

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1,2-Dioleoyl-3-trimethylammonium-propane methyl sulfate salt (DOTAP) was obtained from Avanti Polar Lipids Inc. 1,2-Dioleoyl-sn-glycero-3-phosphoethanolamine (DOPE, COATSOME ME-8181) was obtained from NOF Co. Ltd. Cholesterol (Chol) was purchased from Wako Pure Chemical Industries, Ltd. Quaser670 carboxylic acid was obtained from Biosearch Technologies, Inc. All other chemicals were of the finest grade available.
Hattori Y., Nakagawa Y, & Onishi H. (2019). Gene delivery into hepatic cells with ternary complexes of plasmid DNA, cationic liposomes and apolipoprotein E-derived peptide. Experimental and Therapeutic Medicine, 18(4), 2628-2638.
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