Mda pca 2b

Human Prostate cell lines MDA-PCa-2b, DU-145 were obtained from the American Type Culture Collection (ATCC). Caucasian American (CaA) derived cell line, DU-145 was maintained in RPMI-1640 medium and HPC1 medium was used for the African American (AfA) derived cell line, MDA-PCa-2b. RPMI-1640 and HPC1 media were supplemented with 10% and 20% fetal bovine serum, respectively with 100 IU/mL penicillin and 100 μg/mL streptomycin. Cells were incubated at 37°C in humidified 5% CO₂. To facilitate MDA-PCa-2b attachment, Poly-L-Lysine (Sigma–Aldrich) Cell Attachment Protocol was implemented for all cell culture plates and dishes. These human-derived cell lines were authenticated by DNA short-tandem repeat analysis by the ATCC.

Human prostate cell lines MDA-PCa-2b, DU-145 were obtained from the American Type Culture Collection. The Caucasian derived cell line, DU-145, was maintained in RPMI-1640 with 10% FBS and the African-American derived cell line, MDA-PCa-2b, was cultured in HPC1 with 20% FBS in Poly-L-Lysine (Sigma–Aldrich) coated culture dishes. Culture medium was supplemented with antibiotics and cells were cultured at 37°C with 5% CO₂. African-Americans (n=41) and Caucasians (n=62) clinical FFPE (Formaldehyde Fixed Paraffin Embedded) samples were obtained from the Veterans Affair Medical Center, San Francisco, CA, USA. Additional African-American samples (n=40) were obtained from National Disease Research Interchange. Also, we used miRNA expression data from the Taylor data that is available at the Gene Expression Omnibus (GEO accession number: GSE21032).