Superscript plasmid system cdna library construction kit

Manufactured by Thermo Fisher Scientific

The Superscript plasmid system cDNA library construction kit is a laboratory tool used for the construction of cDNA libraries. It provides the necessary components and protocols for the synthesis and cloning of cDNA into plasmid vectors.

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Lab products found in correlation

Trizol reagent, by Thermo Fisher Scientific (2 mentions) Polyatract mrna isolation system, by Promega (2 mentions) E coli dh5α cells, by National Collection of Authenticated Cell Cultures (1 mentions)

Spelling variants of this product

Superscript Plasmid System (4 citations) CDNA kits (3 citations) CDNA Library Construction Kit (2 citations)

2 protocols using superscript plasmid system cdna library construction kit

Toxin Profiling of H. petersii Venom

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Twenty specimens of H. petersii were collected and milked for 2 days by electrical stimulation. Total RNA was prepared from the glands by using TRIzol reagent (Invitrogen). Poly (A) mRNA was purified by using a Poly (A) Tract mRNA isolation system (Promega). The cDNA library was constructed with the Superscript plasmid system cDNA library construction kit (Gibco/BRL). cDNAs were cloned into the pSPORT1 plasmid (Gibco/BRL) and transformed into Escherichia coli DH5α cells. cDNA clones were randomly chosen and sequenced to obtain a reliable representation of the toxin content in the venom gland. Positive clones were identified by using an ABI Prism 377XL DNA sequencer with a universal T7 promoter primer.

Li Z., Xu X., Meng L., Zhang Q., Cao L., Li W., Wu Y, & Cao Z. (2014). Hp1404, a New Antimicrobial Peptide from the Scorpion Heterometrus petersii. PLoS ONE, 9(5), e97539.

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Scorpion Venom Gland Transcriptome

Cited 1 time

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E. validus scorpions were collected in the Yunnan Province of China. As previously described, the glands were collected 2 days after electrical extraction of the venom 19 (link)-21 (link). Trizol Reagent (Invitrogen) was used to prepare total RNA. Poly(A)-mRNA was purified using a Poly-A Tract mRNA Isolation System (Promega). The cDNA library was constructed according to the specifications of the Superscript Plasmid System cDNA Library Construction Kit (Gibco/BRL). The cDNA was then cloned into pSPORT1 plasmids and transformed into E. coli DH5α cells (China Center for Type Culture Collection, CCTCC). Randomly chosen cDNA clones were sequenced to obtain a reliable representation of the venom gland peptide library.

Zeng Z., Zhang R., Hong W., Cheng Y., Wang H., Lang Y., Ji Z., Wu Y., Li W., Xie Y, & Cao Z. (2018). Histidine-rich Modification of a Scorpion-derived Peptide Improves Bioavailability and Inhibitory Activity against HSV-1. Theranostics, 8(1), 199-211.

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