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Examiner z1 microscope

Manufactured by Zeiss
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Sourced in Germany

The Examiner Z1 microscope is a high-performance laboratory instrument designed for a wide range of applications. It features a versatile optical system, advanced imaging capabilities, and precise control for accurate analysis and observation.

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Lab products found in correlation

Orca flash 4.0 camera, by Hamamatsu Photonics (2 mentions) Opti mem, by Thermo Fisher Scientific (1 mentions) Chicken anti map2, by Abcam (1 mentions)

Spelling variants of this product

Zeiss Axio Examiner Z1 microscope (15 citations) Examiner Z1 (13 citations) LSM 710 Axio Examiner Z1 microscope (3 citations) AXIO Examiner.Z1 confocal microscope (2 citations) Axio Examiner.Z1 upright microscope (2 citations) Examiner Z1 confocal microscope (2 citations) Axio Examiner Z1 fluorescent microscope (2 citations)

5 protocols using examiner z1 microscope

1

Platelet Accumulation at Laser Injury

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Mice (12-16 weeks of age) were anesthetized with ketamine/xylazine (100 mg/kg and 10 mg/kg, respectively) and injected with Alexa Fluor 488-conjugated GPIX antibody (2.5 μg) to label circulating platelets. The saphenous vein was exposed and injured once as previously described [18] . Platelet accumulation at the site of laser injury was assessed by intravital microscopy using a Zeiss Examiner Z1 microscope (Zeiss, Oberkochen,
Germany) equipped with a Hamamatsu Orca Flash 4.0 camera (Hamamatsu, Japan). Image acquisition and fluorescence quantification was done with Slidebook 6.0 (Intelligent Imaging Innovations, Denver, CO).
Paul D.S., Blatt T.N., Schug W.J., Clark E.G., Kawano T., Mackman N., Murcia S., Poe K.O., Mwiza J.M.N., Harden T.K., Bergmeier W, & Nicholas R.A. (2023). Loss of P2Y1 receptor desensitization does not impact hemostasis or thrombosis despite increased platelet reactivity in vitro. Journal of thrombosis and haemostasis : JTH, 21(7).
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2

Multimodal Live-Cell Imaging Workflow

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Images were acquired on a specially designed Zeiss Spinning Disk Confocal Imaging System (Zeiss Examiner Z1 Microscope) using a 63X (wet) objective. The same field of cells was excited with either a 488 nm laser for mCherry or a FITC light source for eGFP and emission filter Em01-R488/568 using constant exposure and intensity settings. Cells were imaged in serum free Opti-MEM® (Life technologies, Carlsbad, CA, USA) supplemented with 10 % fetal bovine serum, 20 mM HEPES pH 7.4, 1 mM sodium pyruvate and 2 mM glutamine. Confocal images were used strictly for presentation in the figures. All data analysis was performed on large fields of cells using the Cellometer® Vision as described above.
Montiel-González M.F., Vallecillo-Viejo I.C, & Rosenthal J.J. (2016). An efficient system for selectively altering genetic information within mRNAs. Nucleic Acids Research, 44(21), e157.
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3

Confocal Microscopy of Bacterial Adhesion

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Samples prepared for confocal microscopy were fixed on silane-coated slides to aid bacterial cell adhesion. Data was acquired on an Axio Examiner Z1 microscope equipped with a Zeiss LSM780 scanhead, using an 63 × oil immersion lens. 405 nm, 488 nm and 633 nm lasers were used to excite the Hoechst nuclear stain, Alexa Fluor 488 and Alexa Fluor 647 conjugates, respectively. Data were collected and the images analysed using Zeiss Zen software.
Smith A.A., Corona-Torres R., Hewitt R.E., Stevens M.P, & Grant A.J. (2022). Modification of avian pathogenic Escherichia coli χ7122 lipopolysaccharide increases accessibility to glycoconjugate antigens. Microbial Cell Factories, 21, 181.
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4

Intravital Imaging of Laser-Induced Platelet Accumulation

Cited 1 time
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Mice (12–14 weeks of age) were anaesthetized with ketamine/xylazine (100 mg/kg and 10 mg/kg, respectively) and injected with Alexa Fluor 488-conjugated antibodies to GPIX (2.5 µg) to label circulating platelets. The saphenous vein was exposed and injured as previously described.22 (link) Platelet accumulation at the site of laser injury was assessed by intravital microscopy using a Zeiss Examiner Z1 microscope (Zeiss, Oberkochen, Germany) equipped with a Hamamatsu Orca Flash 4.0 camera (Hamamatsu, Japan).
Paul D.S., Casari C., Wu C., Piatt R., Pasala S., Campbell R.A., Poe K.O., Ghalloussi D., Lee R.H., Rotty J.D., Cooley B.C., Machlus K.R., Italiano JE J.r., Weyrich A.S., Bear J.E, & Bergmeier W. (2017). Deletion of the Arp2/3 complex in megakaryocytes leads to microthrombocytopenia in mice. Blood Advances, 1(18), 1398-1408.
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5

Neuronal Morphology Analysis on MEAs

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Neuronal morphology on standard MEAs was examined by immunocytochemical staining of microtubule-associated protein 2 (MAP2) (Chicken-anti-MAP2; 1:500; Abcam, United States) expressed in dendrites and somata. Large-scale images spanning the full network (120 × 120 mm) were taken at 10-fold resolution (0.645 pixel/μm; Examiner Z1 microscope, Zen software 2015, Carl Zeiss, Jena, Germany) and processed by background subtraction (750 × 750 pixel median filtering using Zen).
Okujeni S, & Egert U. (2019). Inhomogeneities in Network Structure and Excitability Govern Initiation and Propagation of Spontaneous Burst Activity. Frontiers in Neuroscience, 13, 543.
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