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Iscan coreo slide scaner

Manufactured by Roche
Sourced in United States

The ISCAN Coreo Slide Scanner is a high-performance digital slide scanning system designed for use in research and clinical laboratory settings. It captures high-resolution digital images of microscope slides, enabling efficient storage, analysis, and sharing of sample data.

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5 protocols using iscan coreo slide scaner

1

Immunohistochemical Analysis of FHL2 in Ovarian Tissues

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The expression of FHL2 protein in paraffin-embedded human ovarian tissues and GCTs was detected by a previously described peroxidase-based immunohistochemistry protocol.35 (link) Immunosignals were visualized with a DAB kit (Invitrogen, Grand Island, NY, USA). The sections were counterstained with Mayer's hematoxylin and scanned with an iSCAN Coreo Slide Scaner (Ventana Medical Systems, Inc., Oro Valley, AZ, USA). The positivity (i.e., the number of positively stained cells relative to the total number of cells in the tissue section) and the intensity of the positive immunosignals were quantified with Aperio ImageScope software (Leica Biosystems Inc., Buffalo Grove, IL, USA).
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2

Quantitative Analysis of YAP Expression in Ovarian Tissues

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YAP expression in ovarian tissues was detected using a peroxidase-based immunohistochemistry, the immunosignal was visualized with DAB kit (Invitrogen, Carlsbad, CA). Sections were scanned with an iSCAN Coreo Slide Scaner (Ventana Medical Systems, Inc., Oro Valley, AZ, USA). The intensity of the positive signal was quantified using Aperio ImageScope software (Aperio® Technologies, Inc., Vista, CA).
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3

Immunohistochemical Analysis of YAP Protein

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The expression of YAP protein in paraffin-embedded human ovarian tissues was detected by a previously described peroxidase-based immunohistochemistry protocol (Wang et al. 2012a (link)). Immunosignals were visualized with a 3,3′-diaminobenzidine (DAB) kit (Invitrogen, Carlsbad, CA). The sections were counterstained with Mayer’s hematoxylin. In case of negative controls, the primary antibody was replaced by blocking buffer containing the same amount of IgG from non-immune rabbit serum. Sections were scanned with an iSCAN Coreo Slide Scaner (Ventana Medical Systems, Inc. Oro Valley, AZ). The positivity (i.e., the number of positively-stained cells relative to the total number of cells in the tissue section) and the intensity of the positive immunosignals were quantified with Aperio ImageScope software (Vista, CA).
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4

Quantitative Analysis of YAP Expression in Ovarian Tissues

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YAP expression in ovarian tissues was detected using a peroxidase-based immunohistochemistry, the immunosignal was visualized with DAB kit (Invitrogen, Carlsbad, CA). Sections were scanned with an iSCAN Coreo Slide Scaner (Ventana Medical Systems, Inc., Oro Valley, AZ, USA). The intensity of the positive signal was quantified using Aperio ImageScope software (Aperio® Technologies, Inc., Vista, CA).
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5

Immunohistochemical Analysis of FHL2 in EOC

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To examine the expression of FHL2 in EOC tissues, we performed the peroxidase-based immunohistochemistry as previously described [36 (link)]. Tissue array was commercially purchased from US Biomax Inc. Sections were scanned using an iSCAN Coreo Slide Scaner (Ventana Medical Systems, Inc., Oro Valley, AZ, USA). The intensity of the positive signal was quantified and recorded using Aperio ImageScope software (Aperio Technologies, Inc., Vista, CA, USA).
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