Xrcc4

Manufactured by Horizon Discovery

XRCC4 is a protein that plays a key role in the non-homologous end joining (NHEJ) pathway, a major DNA double-strand break repair mechanism. XRCC4 functions as a scaffold protein, facilitating the recruitment and coordination of other NHEJ factors to repair DNA damage.

Automatically generated - may contain errors

Lab products found in correlation

Sigenome sirna, by Horizon Discovery (1 mentions) On targetplus sirna, by Horizon Discovery (1 mentions) Silencer select sirna, by Thermo Fisher Scientific (1 mentions) Mission sirna, by Merck Group (1 mentions) Brca2, by Horizon Discovery (1 mentions) Hdac1, by Horizon Discovery (1 mentions) Hdac3, by Horizon Discovery (1 mentions) Shc016, by Merck Group (1 mentions) Lipofectamine rnaimax, by Thermo Fisher Scientific (1 mentions)

2 protocols using xrcc4

Targeted RNAi Silencing of Chromatin Regulators

Cited 4 times

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RNAi was performed by the transfection of siRNA oligos using the Lipofectamine RNAiMAX transfection reagent (Thermo Fisher Scientific), according to the manufacturer’s instructions. The sequence of siRNA oligos was as followed.
BRD2 (BRD2, Thermo Fisher Scientific, validated Silencer Select siRNA, ID: s12071). BRD3 (BRD3, Thermo Fisher Scientific, validated Silencer Select siRNA, ID: s15544^{48 (link)}). BRD4_1 (BRD4, Thermo Fisher Scientific, validated Silencer Select siRNA, ID: S23901^{48 (link)}). BRD4_2 (BRD4, Dharmacon, siGENOME siRNA, ID: M-004937-02-0005). BRD4_short (BRD4, Qiagen, ID: SI05044865^{22 (link)}). BRD4_long (BRD4, Qiagen, ID: SI05044872^{22 (link)}). XRCC4_1 (XRCC4, Sigma Aldrich, Mission siRNA, ID: SASI_Hs01_00114717). XRCC4_2 (XRCC4, Dharmacon, ON-TARGET plus siRNA, ID: L-004494-00-0005^{49 (link)}). 53BP1_1 (53BP1, Sigma Aldrich, Mission siRNA, ID: SASI_Hs02_00340027). 53BP1_2 (53BP1, Dharmacon, ON-TARGET plus siRNA, ID: L-003548-00-0005^{50 (link)}).

Wakita M., Takahashi A., Sano O., Loo T.M., Imai Y., Narukawa M., Iwata H., Matsudaira T., Kawamoto S., Ohtani N., Yoshimori T, & Hara E. (2020). A BET family protein degrader provokes senolysis by targeting NHEJ and autophagy in senescent cells. Nature Communications, 11, 1935.

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Targeted Silencing of DNA Repair Genes

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Non-targeting, HDAC1, HDAC2, HDAC3, HDAC6, XRCC4, XRCC5, XRCC6, PARP3, PNKP, BRCA2, MGMT, ELK1 and ELK3 siRNA pools were purchased from GE Healthcare/Dharmacon (D-001810–10, L-003493–00, L-003495–02, L-003496–00, L-003499–00, L-004494–00, L-010491–00, L-005084–00, L-009297–00, L-006783–00, L-003462–00, L-008856–01, L-003885–00, L-010320–00, respectively). SiRNAs were transfected into cells using RNAiMax lipofectamine from Invitrogen. Cells were transfected approximately 24 hours before starting proliferation experiments. Control, HDAC1, HDAC2, HDAC3, and HDAC6 shRNAs were purchased from Sigma-Aldrich (SHC016, SHCLNG-NM_004964, SHCLNG-NM_008229, SHCLNG-NM_010411 and SHCLNG-NM_006044, respectively).

Miles P.D., Yamatani K., Lickley H.L, & Vranic M. (1991). Mechanism of glucoregulatory responses to stress and their deficiency in diabetes.. Proceedings of the National Academy of Sciences of the United States of America, 88(4), 1296-1300.

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