Biotek synergy 2 multi mode reader

Effects of the two most potent compounds, 17c and 17d, on the viability of PANC-1, A2058, Fadu and EBC-1 cancer cells and normal fibroblast cells was measured via the CellTiter-Glo^® luminescent cell viability assay (Promega, Madison, WI, USA), according to the manufacturer’s instructions. Cells were plated onto a flat-bottomed white 96-well plate (BRANDplates, cat. no.: 781965) and were treated with the same method described for the MTT cell viability assay. After the treatment, the luminescence signal was recorded using a microplate reader (BioTek Synergy 2 Multi-Mode Reader, BioTek, Winooski, VT, USA). Dose–response curves (using a nonlinear regression model) were generated, and IC₅₀ values were determined using Graph Pad Prism 8 software (GraphPad Software, San Diego, CA, USA).

Effects of the synthesized compounds on EBC-1 and fibroblast cell viability was measured via CellTiter-Glo^® luminescent cell viability assay (Promega, Madison, WI, USA) according to the manufacturer’s instructions. Briefly, cells were plated at 1000 cells/well onto a flat-bottomed, white 96 well plate (BRANDplates, cat. no.: 781965). After 24 h, cells were treated for 72 h with either the desired 4-fold serial diluted compound concentrations or vehicle (0.2% DMSO) control. After the treatment, the luminescence signal was recorded using a microplate reader (BioTek Synergy 2 Multi-Mode Reader, BioTek, Winooski, VT, USA). Dose–response curves (using a non-linear regression model) were generated and IC₅₀ values were determined using Graph Pad Prism 5.02 software (GraphPad Software, San Diego, CA, USA).