Formvar coated copper grid 400 mesh

The presence of amyloid fibrils in the test samples was monitored by transmission electron microscopy (TEM). End-point solutions of peptides were collected and the samples were dissolved in the same solution to 0.2 mg/mL before studying with electron microscopy. A formvar-coated copper grid 400 mesh (Electron Microscopy Sciences, Hatfield, PA, USA) was placed on a 10 μL sample. After 5 min absorption, the grids with the preparation were negatively stained for 1.5–2.0 min with 1% (weight/volume) aqueous solution of uranyl acetate. The excess of the staining agent was removed with filter paper. The preparations were analyzed using a JEM-100C (Jeol, Tokyo, Japan) transmission electron microscope at the accelerating voltage of 80 kV. Images were recorded on the Kodak electron image film (SO-163) at nominal magnification of 40,000–60,000.

End-point solutions of peptides (0.2 mM in the buffer 50 mM Tris-HCl, pH 7.5, 150mM NaCl, 1% DMSO) incubated for 5 h with ThT were collected, and the samples were dissolved in the same solution to 0.2 mg/mL before studying with electron microscopy according to the previously described methods [55 (link),56 (link)], with minor modifications. A formvar-coated copper grid 400 mesh (Electron Microscopy Sciences, Hatfield, PA, USA) was placed on a 10 μL sample. After 5 min of absorption, the grid with the preparation was negatively stained for 1.5–2.0 min with a 1% (weight/volume) aqueous solution of uranyl acetate. The excess of the staining agent was removed with filter paper. The preparations were analyzed using a JEM-100C (Jeol, Tokyo, Japan) transmission electron microscope at the accelerating voltage of 80 kV. Images were recorded on the Kodak electron image film (SO-163) at a nominal magnification of 40,000–60,000.