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Cryobank

Manufactured by Thermo Fisher Scientific
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Sourced in Singapore

The Cryobank is a laboratory equipment designed for the storage and preservation of samples at ultra-low temperatures. It provides a controlled environment for the long-term storage of a variety of materials such as cells, tissues, and other biological specimens.

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Lab products found in correlation

Sheep blood agar plates, by Thermo Fisher Scientific (1 mentions) Vitek 2 id gn cards, by bioMérieux (1 mentions)

2 protocols using cryobank

1

Isolation and Biobanking of Salmonella and Pasteurella

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Isolates of Salmonella typhimurium and Pasteurella multocida from specimens obtained from the Department of Veterinary Tropical Diseases, University of Pretoria, Idexx Laboratories, Disease Control Africa, Stellenbosch Provincial Veterinary Laboratory, Pathcare Veterinary Laboratories and Vetdiagnostix were all confirmed and bio-banked on beads (Cryobank®, Thermo Fischer) at –70 ºC until it could be processed for MIC and MPC tests (Wentzel 2013 ) (see Table 1).
Wentzel J.M., Biggs L.J, & van Vuuren M. (2022). Comparing the minimum inhibitory and mutant prevention concentrations of selected antibiotics against animal isolates of Pasteurella multocida and Salmonella typhimurium. The Onderstepoort Journal of Veterinary Research, 89(1), 1955.
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2

Isolation and Characterization of CR E. coli

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Nonclonal clinical strains of CR E. coli were previously collected as part of a nationwide surveillance study from 2011 to 2012 and were obtained from the largest tertiary hospital in Singapore (1700 beds). Genus identities were determined using Vitek 2 ID-GN cards (bioMérieux, Inc., Hazelwood, MO, USA). CR E. coli strains were stored at −80 °C in Cryobank (Thermo Scientific, Singapore) storage vials. Fresh isolates were sub-cultured twice on 5% sheep blood agar plates (Thermo Scientific, Singapore) for 24 h at 35 °C before each experiment.
Yeo J.H., Begam N., Leow W.T., Goh J.X., Zhong Y., Cai Y, & Kwa A.L. (2024). Ironing out Persisters? Revisiting the Iron Chelation Strategy to Target Planktonic Bacterial Persisters Harboured in Carbapenem-Resistant Escherichia coli. Microorganisms, 12(5), 972.
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