RAMs were utilized to examine how proteins on the surface of bNPs would be internalized by RAMs. Streptavidin Alexaflour 488 was bound to bNPs by incubation at RT for one hour. Unbound streptavidin was rinsed from bNP as described above. The bNPs were then added to 0.5×106 cells in a 24-well plate and incubated for one hour. The cells were rinsed with sterile PBS to remove excess bNPs and then incubated with an endosome specific fluorescent dye (Lysotracker Deep Red), for 20 minutes at RT. The cells were fixed with 4% formaldehyde and nuclear stained with DAPI. The RAMs were imaged using an Olympus IX73 microscope (Olympus, Center Valley, PA) at 40x magnification. Images were processed using Cellsens Microscope Imaging Software (Olympus). Additionally, RAMS were incubated for one hour with Nile Red bNPs coated with avidin for flow cytometric analysis. Prior to collection, the cells were washed three times with complete medium to remove as much residual bNPs as possible while retained the attached cells. The cells were then suspended in FACS buffer (5% FBS in PBS) and samples were analyzed by Flow Cytometry (Attune NXT) using a red laser to determine Nile Red bNP uptake in RAMs.
Cellsens microscope imaging software
Manufactured by Olympus
Sourced in Japan
CellSens is a microscope imaging software developed by Olympus. It is designed to capture, analyze, and manage images from various Olympus microscope systems. The software provides tools for basic image processing, measurement, and annotation functions.
Automatically generated - may contain errors
Lab products found in correlation
Leica icc50 digital camera, by Leica camera (2 mentions)
Leica dm750 microscope, by Leica camera (2 mentions)
Calcein am fluorescent dye, by Corning (2 mentions)
Fluoroblok 24 well transwell inserts with 8.0 μm colored pet membrane, by Corning (2 mentions)
1x81 inverted fluorescence microscope, by Olympus (2 mentions)
Matrigel matrix, by BD (2 mentions)
Ix73 microscope, by Olympus (2 mentions)
Cytosmart system, by Lonza (1 mentions)
Xm10 camera, by Olympus (1 mentions)
Dapi fluoromount g mounting medium, by Southern Biotech (1 mentions)
Tritc phalloidin, by Yeasen (1 mentions)
Ix83 inverted microscope, by Olympus (1 mentions)
Azure c400 system, by Azure Biosystems (1 mentions)
Roswell park memorial institute (rpmi), by American Type Culture Collection (1 mentions)
Axio observer z1, by Zeiss (1 mentions)
Ficoll pm400, by GE Healthcare (1 mentions)
Imagexpress micro xls system, by Molecular Devices (1 mentions)
Spectra x light engine, by Lumencor (1 mentions)
Metaxpress imaging software, by Molecular Devices (1 mentions)
X cite 120q, by Excelitas (1 mentions)
13 protocols using cellsens microscope imaging software
1
Investigating Nanoparticle Internalization in RAMs
2
Quantifying Myogenic Differentiation Dynamics
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
5 random sample images were collected from each well by investigators that were blind to both condition and time point using an Olympus IX73 microscope and Olympus XM10 camera. Proliferation time points at 6, 24, 48, and 72 hours were used to compare total nuclei to EdU+ nuclei. Differentiation analysis was done at 24 and 72 hours, assessing MyoD+ and Myogenin+ nuclei to total nuclei. For the 6 day analysis, total myotube area was measured and MyHC+ nuclei were compared to total nuclei. Images were analyzed using Olympus cellSens™ microscope imaging software. Additionally, time lapse images were collected every 15 minutes for 12 days using the Lonza CytoSMART™System (Lonza) to analyze HPM differentiation under the 500nM SGI-1252 and vehicle control conditions. HPM plate coverage over the 12-day culture period was measured by the CytoSMART software.
3
Histological Characterization of EpiDerm Tissue
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The EpiDerm™ tissues were pre-fixed overnight at 4 °C in 10% paraformaldehyde. Alcohol gradient dehydration for histological characterization was performed by sequential treatment of 3 samples per group with 30% EtOH for 2 h, 50% EtOH for 2 h, 70% EtOH overnight, 95% EtOH twice for 3 h, and 100% EtOH twice for 1 h each time. Tissue samples were then sectioned and stained using standard HE staining.
The slides were examined and recorded using 20× and 40× magnifications with a Leica DM750 microscope, a Leica ICC50 digital camera, and cellSens microscope imaging software (Olympus Corporation, Warsaw, Poland).
The thickness of the corneal layer and the total EpiDerm™ thickness were defined as indicators of the appropriate activity and function of the model after treatment [19 (link)]. All measurements were performed in randomly selected fields of view at 20× magnification (12 photos per group). For histological examination, 40× magnification was used.
The slides were examined and recorded using 20× and 40× magnifications with a Leica DM750 microscope, a Leica ICC50 digital camera, and cellSens microscope imaging software (Olympus Corporation, Warsaw, Poland).
The thickness of the corneal layer and the total EpiDerm™ thickness were defined as indicators of the appropriate activity and function of the model after treatment [19 (link)]. All measurements were performed in randomly selected fields of view at 20× magnification (12 photos per group). For histological examination, 40× magnification was used.
4
Cardiac Fibrosis Assessment via Masson Trichrome
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Masson trichrome staining was used to evaluate cardiac interstitial fibrosis and structural changes. Per heart, 5 sections (5‐μm thick) were prepared for Masson trichrome staining as per the manufacturer's instructions. Fibrosis was measured via Olympus cellSens Microscope Imaging Software, and determined by fibrosis area/LV area.
5
Immunofluorescence Staining and Microscopy Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The cells were seeded in 24-well plates and allowed to settle overnight. Then, the cells were washed with 1× PBS and fixed with methanol at room temperature for 20 min. After washed with 1× PBS for three times, 0.1% Triton X-100 in 1× PBS was added and incubated at room temperature for 5 min. The cells were then blocked with 2.5% BSA for 1 h at room temperature, followed by incubating with indicated antibodies for 2 h at room temperature. After rinsed with 1× PBS, the cells were incubated with indicated secondary antibody for 1 h at room temperature in the dark. Then, the cells were washed with 1× PBS for three times and added with DAPI Fluoromount-G mounting medium (SouthernBiotech, 0100–20). For F-actin staining, the cells were stained by TRITC-Phalloidin (Yeasen, 40734ES75). The cell nuclei were stained by DAPI Fluoromount-G mounting medium. Immunofluorescence photographs were captured with Olympus IX83 inverted microscope and processed with Olympus CellSens™ Microscope Imaging Software.
6
In Vitro FluoroBlok Invasion Assay
7
Graphene Effects on Duckweed Growth
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Lemna minor was obtained from the Department of Ichthyobiology, Fisheries and Aquaculture Biotechnology, Warsaw, Poland University of Life Sciences. Maintenance and cultivation of fronds were carried out at 20 °C in 6-well plates with Steinberg growth medium [26 (link)] under static conditions. Continuous white fluorescent lighting was used to provide a light intensity from the range of 8500–9500 lx. There were 10 plants per well. Graphene was introduced to the medium at increasing concentrations (5, 10, 20, 50 and 100 μg/mL). After 96 h, frond damage, surface area, biomass and root length were analyzed using a Leica DM750 microscope coupled with a Leica ICC50 digital camera and cellSens microscope imaging software (Olympus Corporation, Warsaw, Poland). The area of the fronds was analyzed by autofluorescence readings using the Azure C400 system (Azure Biosystems, Dublin, Ireland).
8
In Vitro FluoroBlok Invasion Assay
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The in vitro FluoroBlok invasion assay was performed as described in Partridge and Flaherty, 2009 (42 ). FluoroBlok 24 well transwell inserts with 8.0 μm colored PET membrane and Calcein AM fluorescent dye were obtained from Corning (Corning, NY, USA). 300,000 cells were seeded into the upper chamber of the transwell containing 100 μl of 1 mg/ml BD Matrigel matrix (BD Biosciences, San Jose, CA, USA). Fluorescence of invaded cells (relative fluorescence units [RFUs]) was read at 494/517 nm (Ex/Em) wavelengths on a SpectraMax M5 bottom-reading fluorescent plate reader with SoftMax Pro 5 microplate data acquisition and analysis software. Photomicrographs of invaded cells were taken with an Olympus 1X81 inverted fluorescence microscope using Olympus cellSens microscope imaging software.
9
FISH Analysis of BCL2, BCL6, and MYC
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Fluorescence in situ hybridization was performed on 3 μm tissue sections using the BCL2, BCL6, and MYC break apart (split signal) DNA probes (Y5407, Y5408, and Y5410, respectively, Dako K.K., Tokyo, Japan) and MYC/IGH fusion probe (LSI IGH/MYC/CEP 8 Tri-Color Dual Fusion Probe, Vysis, Abbott Molecular, IL, USA). Fluorescence in situ hybridization was performed as previously described. 12, 13 The slides were evaluated using a fluorescence microscope (Olympus BX51, Olympus K.K., Tokyo, Japan), DP73 camera and cellSens microscope imaging software (Olympus). Signals were counted in at least 100 cells and positivity was considered if more than 10% of the tumor cells exhibited a break apart signal for BCL2, BCL6, or MYC, and fusion signal for the MYC/IGH probe.
10
Evaluating STAT5A and S100A4 Inhibition on 4-HC Sensitivity
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
To investigate the inhibition of STAT5A and S100A4 on sensitivity to 4-HC, K-562 cells (5×104 cells/ml) from each experimental group (siControl, siSTAT5A, siS100A4) were treated with 0, 10 or 15 µg/ml 4-HC and incubated for 30 min at 37°C. Following 4-HC treatment, cells were washed twice with chilled culture medium (RPMI; ATCC 30-2001) with 10% fetal bovine serum (FBS; ATCC 30-2020), then plated. K-562 cells were plated in methylcellulose containing 25% FBS or in liquid culture in RPMI containing 10% FBS. Colonies were counted on day 7 of methylcellulose cultures with an inverted microscope (IX53; Olympus Corporation, Tokyo, Japan) under ×10 magnification. A total of three fields were assessed from each group using Olympus CellSens™ Microscope Imaging Software (Olympus Corporation) to count the number of cells. The total number of viable cells in liquid cultures was determined twice within a 7-day period. Viability was determined using trypan blue exclusion criteria.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!