Tetracaine base bp grade

Acetonitrile and methanol both HPLC grade, DPX mounting medium, xylene and Optiphase scintisafe gel were purchased from Fischer Scientific (Leicester, UK). Tetracaine base BP grade (99.9%), formalin solution neutral buffer 10%, DAPI medium, ethanol, heparin sodium salt (I-A), urethane, 0.7% glacial acetic acid, isopropanol and FTIC-dextran with average molecular weight (Mw) of 4 kDa (FD-4) and 10 kDa (FD-10S) used without any further purification steps were supplied by Sigma Aldrich (Dorset, UK). Concentrated hydrochloric acid and sodium hydroxide was from Fluka (Dorset, UK). Sodium acetate was provided by Alfa Aesar (Heysham, UK). Silicone membranes with a thickness of 0.25 mm were purchased from GBUK Healthcare (Selby, UK). Phosphate buffered saline (Dulbecco A) tablets were obtained from Oxiod Limited (Hampshire, England). The Tissue-Tek® O.C.T™ compound, scintillation vials and hydrogen peroxide 30% were obtained from VWR International (Leicestershire, UK). Dextran (carboxyl-¹⁴C) with an average M.W. of 10 kDa and specific activity of 0.00006 Ci/mmol was obtained from American Radiolabeled Chemicals, Inc. (St. Louis, USA). Soluene® 350 was provided by Perkin Elmer (Bucks, UK). Isoflurane 100% (w/v) inhalation vapour liquid was obtained from Animal Care Ltd. (York, UK).

Acetonitrile and methanol both HPLC grade, grade A glass pipettes, clear glass high performance liquid chromatography (HPLC) vials with crimpable lids and 0.45 μm nylon filter papers were purchased from Fischer Scientific (Leicester, UK). Tetracaine base BP grade (99.9%) and deuterium oxide (99.9 atom%) were supplied by Sigma Aldrich (Dorset, UK). Concentrated hydrochloric acid and sodium hydroxide were from Fluka (Dorset, UK). Sodium acetate, potassium dihydrogen phosphate and 1-Octanol were provided by Alfa Aesar (Heysham, UK). Silicone membranes with a thickness of 0.25 mm were purchased from GBUK Healthcare (Selby, UK).