Antibodies against cleaved parp and hif 1α

Antibodies against EGFR, Akt, MEK1/2 and tubulin were purchased from Cell Signaling Technology (Danvers, MA, USA). Antibodies against cleaved PARP and HIF-1α were purchased from BD Biosciences (San Jose, CA, USA). Antibodies against IGF-1R and actin were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA). HRP-conjugated anti-rabbit and anti-goat secondary antibodies were purchased from GeneTex (Irvine, CA, USA). An anti-Hsp90 antibody was purchased from Enzo Life Science (Farmingdale, NY, USA). Ni-NTA agarose was purchased from Invitrogen (Carlsbad, CA, USA). ATP-agarose was acquired from Innova Biosciences (Cambridge, UK). The first-strand cDNA synthesis kit was purchased from Dakara Korea Biomedical, Inc. (Seoul, Republic of Korea). 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), propidium iodide (PI), crystal violet, and other chemicals were purchased from Sigma-Aldrich (St. Louis, MO, USA) unless otherwise specified.

Antibodies against pAkt (S473), Akt, pSrc (Y416), Src, pMEK (S217/221), MEK, Nanog, and cleaved caspase-3 were purchased from Cell Signaling Technology (Danvers, MA, USA). Antibodies against cleaved PARP and HIF-1α and Matrigel were purchased from BD Biosciences (San Jose, CA, USA). Primary antibodies against 6x-His tag, Ubiquitin, and Actin were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Antibodies against HSP70, HSP90, and Hop were purchased from Enzo Life Science (Farmingdale, NY, USA). Antibodies against Oct4 and Sox2 were purchased from Abcam (Cambridge, UK). Horseradish peroxidase (HRP)-conjugated secondary antibodies were purchased from GeneTex (Irvine, CA, USA). Ni-NTA agarose and fluorescence (Alexa Fluor 488 and Alexa Fluor 594)-conjugated secondary antibodies was purchased from Thermo Fisher Scientific. Biotinylated secondary antibodies were purchased from Bethyl Laboratories (Montgomery, TX, USA). ATP-agarose was acquired from Innova Biosciences (Cambridge, UK). Propidium iodide (PI), 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT), and other chemicals were purchased from Sigma-Aldrich (St. Louis, MO, USA) unless otherwise specified. The detailed information on used primary and secondary antibodies, including vendor, catalogue number, application, and dilution ratio (or concentration) is listed in Table S2.