For transient depletions, we used ON-TARGET Plus RNAi duplexes from Dharmacon. Control Negative Silencer® siRNA was from Ambion. RNAi duplexes were transfected using Lipofectamine RNAiMax (Invitrogen). After 48 h cells were serum starved where specified and used in the indicated experiments.
Control negative silencer sirna
Manufactured by Thermo Fisher Scientific
Control Negative Silencer® siRNA is a synthetic double-stranded RNA molecule designed to serve as a negative control for siRNA experiments. It is used to assess the specificity of target gene silencing by RNA interference (RNAi) without affecting the expression of any endogenous genes.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using control negative silencer sirna
1
Transient Protein Depletion Assay
2
Transient Protein Depletion Assay
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