Control sirna

Manufactured by Eurofins

Control siRNA is a laboratory reagent used as a reference in RNA interference (RNAi) experiments. It serves as a negative control to help determine the specific effects of target-specific siRNAs. Control siRNA does not target any known gene or transcript, allowing researchers to assess non-specific effects in their experiments.

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Control siRNA-1 SiRNAs

6 protocols using control sirna

Silencing DNA repair genes in cells

Cited 3 times

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The control siRNA (5’-UAAUGUAUUGGAACGCAUA-3’), BLM siRNA (5’-CCGAAUCUCAAUGUACAUAGA-3’)65 (link), RECQ5 siRNA (5’-CAGGAGGCUGAUAAAGGGUUA-3’)65 (link), TREX1 siRNA (5’-CCAAGACCAUCUGCUGUCA-3’)66 (link), CTIP siRNA (5’-GCUAAAACAGGAACGAAUC-3’)67 (link), GEN1 siRNA (5’-GUAAAGACCUGCAAUGUUA-3’) were purchased from Eurofins. The MUS81 siRNA (5’-CAGCCCUGGUGGAUCGAUA-3’ and 5’-CAUUAAGUGUGGGCGUCUA-3’)68 (link), SLX1 siRNA (5’-UGGACAGACCUGCUGGAGAUU-3’)69 (link), ERCC1 siRNA (SMARTpool ON-TARGET plus L-006311), MRE11 siRNA (SMARTpool ON-TARGET plus L-009271), EXO1 siRNA (SMARTpool ON-TARGET plus L-013120), DNA2 siRNA (SMARTpool ON-TARGET L-026431), RECQ1 siRNA (SMARTpool ON-TARGET plus L-013597), RECQ4 siRNA (SMARTpool ON-TARGET plus L-010559), WRN siRNA (SMARTpool ON-TARGET plus L-010378), BRCA2 siRNA (SMARTpool ON-TARGET plus L-003462) and RAD51 siRNA (SMARTpool ON-TARGET plus L-003530) were purchased from Dharmacon. Cells were seeded one day before siRNA treatment and transfected with 25 nM of siRNA using Lipofectamine RNAiMAX (Life Technologies).

Chan Y.W., Fugger K, & West S.C. (2017). Unresolved recombination intermediates lead to ultra-fine anaphase bridges, chromosome breaks and aberrations. Nature cell biology, 20(1), 92-103.

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Silencing DNA repair genes in cells

Cited 3 times

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Chan Y.W., Fugger K, & West S.C. (2017). Unresolved recombination intermediates lead to ultra-fine anaphase bridges, chromosome breaks and aberrations. Nature cell biology, 20(1), 92-103.

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JAK1/JAK2 Silencing in A549 Cells

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Human JAK1 siRNA (5′-GCCUGAGAGUGGAGGUAA-3′), human JAK2 siRNA (5′-GAACAGGAUUUACAGUUA-3′) as well as control siRNA (5′-UUCUCCGAACGUGUCACGU-3′) were synthesized by Eurofins Genomics GmbH. Transfection of A549 cells was performed with Lipofectamine 2000 (Invitrogen) according to the manufacturer's protocols.

Mecate-Zambrano A., Sukumar S., Seebohm G., Ciminski K., Schreiber A., Anhlan D., Greune L., Wixler L., Grothe S., Stein N.C., Schmidt M.A., Langer K., Schwemmle M., Shi T., Ludwig S, & Boergeling Y. (2020). Discrete spatio-temporal regulation of tyrosine phosphorylation directs influenza A virus M1 protein towards its function in virion assembly. PLoS Pathogens, 16(8), e1008775.

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Modulation of Endothelial Cell Phenotype

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SNAI1, SNAI2 and ZEB2 siRNAs were purchased from Eurogentec. Control siRNA was purchased from Eurofins (Supplemental Table 3). A non-relevant siRNA (Ctrl). Cells were transfected with siRNAs using the calcium phosphate method into 100-mm dishes containing 1 × 10⁶ HMVECs. After 6 h of transfection, cells were washed and the day after a new transfection was performed. After 48 h, transfected cells were treated for 48 h or 72 h with TNF-α (100 ng/ml; Sigma) and analysed as described hereunder.

Adjuto-Saccone M., Soubeyran P., Garcia J., Audebert S., Camoin L., Rubis M., Roques J., Binétruy B., Iovanna J.L, & Tournaire R. (2021). TNF-α induces endothelial–mesenchymal transition promoting stromal development of pancreatic adenocarcinoma. Cell Death & Disease, 12(7), 649.

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Transfection of KIF5A siRNA

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Cells were transfected with ON-TARGET plus KIF5A siRNA SMART pool (Dharmacon) or control siRNA (Eurofins) for 4.5 hours at 37 °C in SFM using Lipofectamin 2000 (Invitrogen), then were maintained in LHC8 complete medium and used in the following experiments.

Kamata H., Tsukasaki Y., Sakai T., Ikebe R., Wang J., Jeffers A., Boren J., Owens S., Suzuki T., Higashihara M., Idell S., Tucker T.A, & Ikebe M. (2017). KIF5A transports collagen vesicles of myofibroblasts during pleural fibrosis. Scientific Reports, 7, 4556.

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Transfection of CETP siRNA in Cells

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6 × 10⁵ cells were transfected with 40nmolCETP siRNA (Applied Biosystems) or Control siRNA (Eurofins) using the FuGene HD (Promega) reagent as per the manufacturer's instructions. Briefly, cells were trypsinized with 2 ml of trypsin, and 4 ml of complete media was added to deactivate the trypsin. Cell numbers were determined and 6 × 10⁵ cells were aliquoted into 2 ml of media. The transfection cocktail containing 30 μl of the FuGene HD and either 4 μl of the control or CETP siRNA (40 nmol) in 500 μl of serum-free media was added dropwise to cells. Cells were cultured in 6- or 96- well plates for 72 h before further analysis was performed.

Esau L., Sagar S., Bangarusamy D, & Kaur M. (2016). Identification of CETP as a molecular target for estrogen positive breast cancer cell death by cholesterol depleting agents. Genes & Cancer, 7(9-10), 309-322.

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