Plasmodium vivax genomic DNA was extracted from 200 μl of each infected blood sample using DNA blood kit following the manufacturer’s instructions (TaKaRa, Japan) with minor modifications. The DNA was dissolved in TE buffer (10 mM Tris–HCl, pH 8.0, 0.1 M EDTA) and stored at -20°C until use. The quality of total DNA was analysed by running 5 μL of each DNA sample on a 1.0% agarose gel stained with ethidium bromide and visualized with UV illumination.
Dna blood kit
Manufactured by Takara Bio
Sourced in Japan
The DNA blood kit is a laboratory product designed to extract and purify DNA from blood samples. It provides a simple and efficient method for isolating DNA, which is a fundamental component for various genetic analysis and research applications.
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Lab products found in correlation
4 protocols using dna blood kit
1
Plasmodium vivax DNA Extraction
2
Genomic DNA Extraction from Whole Blood
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Genomic DNA was extracted from 100 μL of each whole blood sample using DNA blood kit according to the manufacturer’s protocol (Takara, Japan). The extracted DNA was eluted in 60 μL of TE buffer (10 mM Tris–HCl, 0.1 M EDTA, pH 8.0) and stored at −20 °C until use. The quality of genomic DNA was detected using 1.0 % agarose gel electrophoresis and goldviewer buffer staining used as ethidium bromide substitute (Sangon Bio Inc., Shanghai, China).
3
Plasmodium vivax DNA Extraction Protocol
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Plasmodium vivax genomic DNA was extracted from 200 μL of each blood sample using a DNA blood kit following the manufacturer’s instructions (Takara Bio Inc., Shiga, Japan) with minor modifications. The DNA was dissolved in TE buffer (10 mM Tris–HCl, 0.1 M EDTA, pH 8.0) and stored at -20°C until use. The quality of total DNA was analysed by running 5 μL of each DNA sample on a 1.0% agarose gel stained with ethidium bromide and visualized under ultraviolet illumination.
4
Extracting Genomic DNA from Malaria Parasites
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Genomic DNA of parent strains (sensitive P. berghei K173) and drug-resistant strains (ART- and PQ-resistant strains of the 30th and 50th generation) was extracted from 100 μL of each whole blood sample using DNA blood kit according to the manufacturer’s protocol (Takara Bio Inc. Nojihigashi 7-4-38, Kusatsu, Shiga 525–0058, Japan, Lot No.: AK1901). The extracted DNA was eluted in 60 μL of TE buffer (10 mM Tris–HCl, 0.1 M EDTA, pH 8.0) and stored at −20°C until further use. The quality of genomic DNA was detected using 1.0% agarose gel electrophoresis. The gel was stained with Goldview nucleic acid stain used as an ethidium bromide substitute (Sangon Bio Inc., Shanghai, China).
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