β actin sc 47778hrp

ABA (A0792) was purchased from Tokyo Chemical Industry, ThS (T1892), anhydrous dimethyl sulfoxide (DMSO), triton X-100, paraformaldehyde (PFA), phosphate buffer (PB), phosphate-buffered saline (PBS, pH 7.4), and tris-buffered saline (TBS) were purchased from Sigma-Aldrich (St. Louis, MO, USA). RIPA buffer (89901) and protease/phosphatase inhibitor cocktail (78445) were obtained from Thermo Fisher Scientific (Waltham, MA, USA). Fluorescence mounting medium (S3023) was purchased from Dako (Santa Clara, CA, USA). Primary antibodies against Aβ (6E10; 803001, BioLegend, San Diego, CA, USA), PGC-1α (sc-518025, Santa Cruz Biotechnology, Dallas, TX, USA), PS1 (5643s, Cell Signaling Technology, Danvers, MA, USA), PPAR-γ (2443s, Cell Signaling Technology), p-CREB (9198s, Cell Signaling Technology), CREB (9197s, Cell Signaling Technology), IDE (ab32216, Abcam, Cambridge, MA, USA), LANCL2 (MBS154355, MyBioSource, San Diego, CA, USA), GFAP (Z0334, Dako), Iba-1 (019-19741, Wako Chemical, Osaka, Japan), and NEP (AF1126, R&D Systems, Minneapolis, MN, USA) were used. β-actin (sc-47778HRP, Santa Cruz Biotechnology) and horseradish peroxidase (HRP)-conjugated secondary antibodies were purchased from Santa Cruz Biotechnology. Alexa Fluor secondary antibodies were purchased from Thermo Fisher Scientific.

Western blot analysis was performed as described previously (16 (link)). Briefly, samples were separated on SDS–polyacrylamide gels and transferred to nitrocellulose membranes. The membranes were incubated with corresponding antibodies. Immunoreactive bands were visualized using the ChemiDoc™ Imaging System (Bio-Rad, USA) with a Super Signal™ West Pico PLUS Chemiluminescent Substrate Kit (Thermo). The primary antibodies included anti-LDHB (ab53292, Abcam, USA), anti-LDHA (66287-1-Ig, Proteintech, China), anti-Flag (A8592, Sigma, USA), anti-Myc (M047-7, MBL, USA), β-actin (sc-47778HRP, Santa Cruz, USA), anti-PKM2 (15822-1-AP, Proteintech, China), anti-HK2 (66974-1-Ig, Proteintech, China), anti-PGK1 (17811-1-AP, Proteintech, China), anti-ENO1 (11204-1-AP, Proteintech, China), anti-TERT for WB (ab32020, Abcam, USA), anti-TERT for IP (abx120550, Abbexa, United Kingdom).