Patch clamp microscope

Manufactured by Olympus

Sourced in Japan

The Patch Clamp Microscope is a specialized scientific instrument used to measure the electrical activity of individual cells. It combines a microscope and a patch clamp amplifier to enable the recording of ionic currents, membrane potentials, and other electrophysiological properties of cells. The microscope provides a high-magnification view of the sample, allowing for precise positioning of the patch clamp pipette onto the target cell.

Automatically generated - may contain errors

Lab products found in correlation

Axon multiclamp 700b amplifier, by Molecular Devices (1 mentions)

2 protocols using patch clamp microscope

Electrophysiology of iPSC-derived Neurons

Check if the same lab product or an alternative is used in the 5 most similar protocols

iPSC-derived neurons were tested on a microscopic workbench with a patch clamp. Neurons were immersed in extracellular fluid containing 95% O₂ and 5% CO₂ during the whole process. Cells were visualized using an Olympus patch clamp microscope (Olympus Corporation, Tokyo, Japan). Medium-sized neurons with a bright cell margin and smooth surface were selected for patch clamp analysis. Neurons were inserted into the clamp and data were recorded using an Axon MultiClamp 700B amplifier (Axon instruments; Molecular Devices, LLC, Sunnyvale, CA, USA) and Igor 5.0 software (Wavemetrics, Inc., Lake Oswego, OR, USA).

Lin C., Liu C., Zhang L., Huang Z., Zhao P., Chen R., Pang M., Chen Z., He L., Luo C., Rong L, & Liu B. (2017). Interaction of iPSC-derived neural stem cells on poly(L-lactic acid) nanofibrous scaffolds for possible use in neural tissue engineering. International Journal of Molecular Medicine, 41(2), 697-708.

+ Open protocol

+ Expand

Patch Clamp Analysis of iNSC-derived Neurons

Check if the same lab product or an alternative is used in the 5 most similar protocols

iNSC-derived neurons were tested on a microscopic workbench with a patch clamp. Neurons were immersed in extracellular fluid containing 95% O₂ and 5% CO₂ during the whole process. Cells were visualized using an OLYMPUS patch clamp microscope. Medium-sized neurons with a bright cell margin and smooth surface were selected for patch clamp analysis. Neurons were inserted into the clamp and stimulated by an electric current and data were recorded using and AXON MultiClamp 700B amplifier and Igor 5.0 software.

Liu C., Huang Y., Pang M., Yang Y., Li S., Liu L., Shu T., Zhou W., Wang X., Rong L, & Liu B. (2015). Tissue-Engineered Regeneration of Completely Transected Spinal Cord Using Induced Neural Stem Cells and Gelatin-Electrospun Poly (Lactide-Co-Glycolide)/Polyethylene Glycol Scaffolds. PLoS ONE, 10(3), e0117709.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!