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Silastic tubes

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Silastic tubes are flexible, non-reactive silicone-based tubes used in various laboratory applications. They are designed to withstand a range of temperatures and chemical exposures, making them suitable for fluid transfer, sampling, and other experimental setups.

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Lab products found in correlation

Silastic laboratory tubing, by Dow (1 mentions) Testosterone, by Steraloids (1 mentions) Rtv silicone sealant, by Dow (1 mentions) 17 β estradiol 3 benzoate, by Merck Group (1 mentions) 17β estradiol, by Merck Group (1 mentions) Isoflurane, by Phoenix Pharmaceuticals (1 mentions)

5 protocols using silastic tubes

1

Androgenizing Female Rats via Testosterone Implants

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One group of adult female rats was ovariectomized, 1 week after which they received testosterone implants. These remained in place for an additional 1 week, at which time i.t. cannulation and perfusion (see below) were performed. This method, previously used to androgenize gonadectomized adult rats, achieves circulating testosterone levels comparable to those of intact adult male rats (11.7 ± 4.7 ng/ml).70 (link),101 (link) In brief, Silastic laboratory tubing (Dow Corning, Midland, MI) was used for testosterone implants, which consisted of two Silastic tubes (i.d. 0.20 cm, o.d. 0.32 cm, length 3 cm) packed with testosterone (Steraloids, Inc., Newport, RI) and sealed with A-100 Medical Silicone Adhesive (Factor II, Inc., Lakeside, AZ). Implants were submerged in 0.9% saline solution at 37°C for 24 ho urs, and then implanted subcutaneously 3 cm distal to the shoulder blade (under 2.5% isoflurane anesthesia).
Kumar A., Liu N.J., Madia P.A, & Gintzler A.R. (2015). Contribution of Endogenous Spinal Endomorphin 2 to Intrathecal Opioid Antinociception in Rats Is Agonist-Dependent and Sexually Dimorphic. The journal of pain : official journal of the American Pain Society, 16(11), 1200-1210.
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2

Silastic Tube Testosterone Implantation

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To stimulate hyperandrogenemia, the silastic tubes (I.D. 1.98 mm × O.D. 3.18 mm; Dow Corning, Midland, MI, USA) were used along with the implantable osmotic pump (Durect Corporation, Curpertino, CA, USA) filled with DHT as described earlier [18 (link)]. The osmotic pump releases the controlled, predetermined level of DHT (3.46 µg/h) based upon the osmotic pressure difference between the osmotic layer and the tissue environment.
Krishnan A., Muthusami S., Periyasamy L., Stanley J.A., Gopalakrishnan V, & Ramachandran I. (2020). Effect of DHT-Induced Hyperandrogenism on the Pro-Inflammatory Cytokines in a Rat Model of Polycystic Ovary Morphology. Medicina, 56(3), 100.
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3

Silastic Implants for Hormone Delivery

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To minimize the stress of injections and the fluctuations in plasma levels of hormones associated with bolus steroid injections, hormones were replaced using Silastic implants. These are prepared according to the method described by Legan et al.(1975) (link), and modified according to Febo et al.(2002) (link). The Silastic tubes (Dow Corning, Midland, MI, USA) had an internal diameter of 1.47 mm and an external diameter of 1.97 mm (“membrane thickness” of 0.5 mm). For estradiol administration, the length of the Silastic tubes was 5 mm (volume = 8.49 mm3). For DHT administration we used 2 Silastic implants, each measuring 10 mm in length (total volume = 16.97 mm3). Implants were empty (GDX) or filled with gonadal steroids and sealed on both ends with Room Temperature Vulcanizing (RTV) Silicone Sealant (Dow Corning, Midland, MI, USA). Hormones were purchased from Sigma-Aldrich (St. Louis, MO, USA). Implants were placed subcutaneously in the midscapular region during surgery and remained there throughout the experiments. Silastic implants were used instead of commercially available steroid pellets. Our previous studies agree with that of others that report a better and more consistent release from Silastic tubes (Strom et al. 2008; Ingberg et al. 2012 (link)) than from commercially available pellets.
Menéndez-Delmestre R., Agosto-Rivera J.L., González-Segarra A.J, & Segarra A.C. (2024). Cocaine sensitization in male rats requires activation of estrogen receptors. bioRxiv.
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4

Estradiol-Filled Silastic Implant Protocol

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Silastic tubing implants were prepared according to Legan et al. and modified according to Febo et al. [18 (link),19 (link)]. Briefly, 5 mm Silastic tubes (1.47 mm internal diameter, 1.97 mm outside diameter; Dow Corning, distributed by Fisher Scientific, Cayey, Puerto Rico) were filled with 3, 4 and 5 mg of 17-β-estradiol 3-benzoate (Sigma-Aldrich, St. Louis, MO, USA) or left empty. These tubes were sealed at each end with sterile silicone adhesive sealant. Silastic implants were placed in a 0.9% sterile saline solution 3 hrs prior to use to confirm the integrity of the tubes. Those that did not float at the end of this period were discarded. Commercial pellets of 17-β-estradiol (3 mg and 4 mg) were purchased from Innovative Research (Sarasota, FL) to compare its delivery efficiency with that of the estradiol-filled Silastic tubing. Placebo pellets composed of cholesterol and other “inert” ingredients (personal communication) were purchased to use as controls. All implants were inserted in the midscapular region immediately after ovariectomy to reduce alterations in the physiology of the animal, such as the increase in appetite, weight and anxiety among others.
Mosquera L., Shepherd L., Torrado A.I., Torres-Diaz Y.M., Miranda J.D, & Segarra A.C. (2015). Comparison of Two Methods of Estradiol Replacement: their Physiological and Behavioral Outcomes. Journal of veterinary science & technology, 6(6), 276-.
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5

Estrogen Administration in Male Rats

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Chronic administration of estrogen in male rats was performed as described previously by Smith and colleagues (Smith et al., 1977 ). Briefly, 17β-estradiol [from Sigma-Aldrich (St. Louis, MO, USA)] was administered by implanting estrogen filled Silastic tubes [(I.D. 1.57 mm, O.D. 3.18 mm) of 5 mm effective length; Dow Corning, Midland, MI, USA)]. The ends of the implants were sealed with silicone rods (Goodfellow, Cambridge, UK). Implants were then washed in ethanol and equilibrated in four changes of warm phosphate-buffered saline over a 24-h period before placement in the rat. Under inhalation anesthesia, isoflurane (2.5%; Phoenix Pharmaceuticals, St. Joseph, MO, USA) in 97.5% O2, six male rats were treated with 0.5% of bupivacaine at the incision site and an intramuscular injection of carprofen (5 mg/kg). Implants were placed subcutaneously on the rat’s back, at the time of surgery (Smith et al., 1977 ). Implants were placed 2 weeks before sumatriptan (1 ng) was injected intradermally on the dorsum of the hindpaw.
Araldi D., Ferrari L.F., Green P, & Levine J.D. (2016). Marked Sexual Dimorphism in 5-HT1 Receptors Mediating Pronociceptive Effects of Sumatriptan. Neuroscience, 344, 394-405.
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