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Brilliant violet 421 anti mouse cd326 ep cam

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Brilliant Violet 421 anti-mouse CD326 (Ep-CAM) is a fluorochrome-conjugated antibody used for the detection and analysis of mouse CD326 (Ep-CAM) expression by flow cytometry.

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Lab products found in correlation

Facsaria 2, by BD (2 mentions) Percp cy5.5 rat anti mouse cd45, by BD (2 mentions) Pe cy7 rat anti mouse cd31, by BD (2 mentions) Ebioscience fixable viability dye efluor 780, by Thermo Fisher Scientific (2 mentions)

Close spelling variants of this product

Brilliant Violet 421 (66 citations)

2 protocols using brilliant violet 421 anti mouse cd326 ep cam

1

Isolation of Lung Cell Populations

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Single cell suspensions of whole lungs were prepared as described previously [83 (link)]. For FACS, single cell suspensions were incubated with eBioscience Fixable Viability Dye eFluor™ 780 (Invitrogen) and the following antibodies (0.6 μg per 107 cells): PerCP-Cy5.5 Rat Anti-Mouse CD45 (BD Pharmingen, 550994), PE-Cy7 Rat Anti-Mouse CD31 (BD Pharmingen, 561410), and Brilliant Violet 421 anti-mouse CD326 (Ep-CAM) (BioLegend, 118225) on ice for 45 min, and then subjected to FACS-sorting using FACS Aria II (BD Biosciences) at the Moody Foundation Flow Cytometry Core Facility at the Children’s Research Institute at UTSW. Flow cytometry data were analyzed with FlowJo v10.
Kasiri S., Chen B., Wilson A.N., Reczek A., Mazambani S., Gadhvi J., Noel E., Marriam U., Mino B., Lu W., Girard L., Solis L.M., Luby-Phelps K., Bishop J., Kim J.W, & Kim J. (2020). Stromal Hedgehog pathway activation by IHH suppresses lung adenocarcinoma growth and metastasis by limiting reactive oxygen species. Oncogene, 39(16), 3258-3275.
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2

Lung Single Cell Sorting for FACS

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Single cell suspensions of whole lungs were prepared as described previously [84 (link)]. For FACS, single cell suspensions were incubated with eBioscience Fixable Viability Dye eFluor™ 780 (Invitrogen) and the following antibodies (0.6 μg per 107 cells): PerCP-Cy5.5 Rat Anti-Mouse CD45 (BD Pharmingen, 550994), PE-Cy7 Rat Anti-Mouse CD31 (BD Pharmingen, 561410), and Brilliant Violet 421 anti-mouse CD326 (Ep-CAM) (BioLegend, 118225) on ice for 45 minutes, and then subjected to FACS-sorting using FACS Aria II (BD Biosciences) at the Moody Foundation Flow Cytometry Core Facility at the Children’s Research Institute at UTSW. Flow cytometry data were analyzed with FlowJo v10.
Kasiri S., Chen B., Wilson A.N., Reczek A., Mazambani S., Gadhvi J., Noel E., Marriam U., Mino B., Lu W., Girard L., Solis L.M., Luby-Phelps K., Bishop J., Kim J.W, & Kim J. (2020). Stromal Hedgehog pathway activation by IHH suppresses lung adenocarcinoma growth and metastasis by limiting reactive oxygen species. Oncogene, 39(16), 3258-3275.
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