The clinically isolated MRSA strain and the methicillin-sensitive ATCC29213 strain provided by the Department of Laboratory Medicine, West China Hospital, were applied [6 (link)]. According to our previous protocol, S. aureus strains were cultured in TSB medium to midexponential phase (OD600 = 0.5). For biofilm formation, five hundred microliters of S. aureus suspension (OD600 = 0.5) were dropped in 24-well polystyrene culture plates (Nest Biotechnology, Wuxi, Jiangsu, China) and cultured with 14-mm-diameter glass cover slips (Nest Biotechnology, Wuxi, Jiangsu, China) for 24 h for biofilm formation [1 (link)].
24 well polystyrene culture plates
Manufactured by NEST Biotechnology
Sourced in China
24-well polystyrene culture plates are a type of laboratory equipment used for the in vitro cultivation of cells. These plates have a flat-bottom design with 24 individual wells, each capable of holding a small volume of cell culture media and samples. The plates are made of polystyrene, a common material used in cell culture applications due to its optical clarity and compatibility with various cell types.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using 24 well polystyrene culture plates
1
MRSA and MSSA Biofilm Formation
2
MRSA Biofilm Transformation with GO-PEI-AsyycG
Check if the same lab product or an alternative is used in the 5 most similar protocols
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A single colony of MRSA was selected from tryptose soya agar (TSA) plates and cultured in
liquid TSB medium to an OD600 value of 0.5, representing mid-exponential
growth. Two hundred and fifty microliters of the mid-exponential phase MRSA cultures were
placed in 24-well polystyrene culture plates (Nest Biotechnology, Wuxi, Jiangsu, China),
and a biofilm was established on glass cover slips (14 mm in diameter) (Nest
Biotechnology, Wuxi, Jiangsu, China) by culturing for 24 hr. The biofilms were then washed
three times with PBS buffer to remove planktonic bacteria before the addition of
transformation reagents. For the GO group, 2 µl of GO solution (with a
concentration of 50 mg/ml determined by cell viability assay) was added
to 24 hr old MRSA biofilms. For the ASyycG group, 2 µlof recombinant pDL278 ASyycG plasmid was added to the MRSA biofilm
according to our previous methods. For GO-PEI-ASyycG transformations, 2
µl of the GO-PEI-ASyycG solution, which already
included 200 ng of ASyycG plasmids, was added to 24 hr
MRSA biofilms. After intervention, all the MRSA biofilms were cultured at 37°C containing
5% CO2 for an additional 24 hr and prior to further investigation [32 (link)].
liquid TSB medium to an OD600 value of 0.5, representing mid-exponential
growth. Two hundred and fifty microliters of the mid-exponential phase MRSA cultures were
placed in 24-well polystyrene culture plates (Nest Biotechnology, Wuxi, Jiangsu, China),
and a biofilm was established on glass cover slips (14 mm in diameter) (Nest
Biotechnology, Wuxi, Jiangsu, China) by culturing for 24 hr. The biofilms were then washed
three times with PBS buffer to remove planktonic bacteria before the addition of
transformation reagents. For the GO group, 2 µl of GO solution (with a
concentration of 50 mg/ml determined by cell viability assay) was added
to 24 hr old MRSA biofilms. For the ASyycG group, 2 µlof recombinant pDL278 ASyycG plasmid was added to the MRSA biofilm
according to our previous methods. For GO-PEI-ASyycG transformations, 2
µl of the GO-PEI-ASyycG solution, which already
included 200 ng of ASyycG plasmids, was added to 24 hr
MRSA biofilms. After intervention, all the MRSA biofilms were cultured at 37°C containing
5% CO2 for an additional 24 hr and prior to further investigation [32 (link)].
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