Rabbit anti erα

Manufactured by Merck Group

Sourced in United States

Rabbit anti-ERα is a primary antibody that specifically binds to the estrogen receptor alpha (ERα) protein. It can be used in various laboratory applications, such as Western blotting, immunohistochemistry, and immunoprecipitation, to detect and study the expression and localization of ERα in biological samples.

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Lab products found in correlation

Rabbit anti ar, by Abcam (3 mentions) Mg132, by Merck Group (1 mentions) Anti flag, by Merck Group (1 mentions) 17β estrogen e2, by Abcam (1 mentions) Rabbit anti myc, by Merck Group (1 mentions) Mouse anti flag m2, by Merck Group (1 mentions) Mouse anti src 3, by BD (1 mentions) Goat anti choline acetyltransferase chat, by Merck Group (1 mentions) Anti mouse af546, by Thermo Fisher Scientific (1 mentions) Mouse anti calcitonin gene related peptide, by Abcam (1 mentions) Alexa fluor 488 antibody, by Thermo Fisher Scientific (1 mentions) Chicken anti gfp primary antibody, by Aves Labs (1 mentions) Rabbit anti pr, by Agilent Technologies (1 mentions) Alexafluor568 antibody, by Thermo Fisher Scientific (1 mentions) Optical microscope, by Olympus (1 mentions) Goat anti chat, by Merck Group (1 mentions) Ctb 488, by Thermo Fisher Scientific (1 mentions) Ctb 555, by Thermo Fisher Scientific (1 mentions)

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Anti-ERα (2 citations) Rabbit anti- (2 citations)

6 protocols using rabbit anti erα

PES1 Modification and Interaction Assays

Cited 2 times

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Rabbit anti-Myc, anti-Flag, mouse anti-Flag (M2) and cycloheximide (CHX) were obtained from Sigma. Rabbit GFP was purchased from GeneTex. Rabbit anti-PES1and 17β-estrogen (E2) were obtained from Abcam. Rabbit anti-ERα was obtained from Millipore. MG132 was purchased from Merk. The siRNA sequences, siTrim23 and si control, were obtained from GenePharma RNAi Company and their sequences are shown in Supplementary Table S1.
HA-PES1 was obtained from Dr. Dirk Eick (Institute of Clinical Molecular Biology and Tumor Genetics, GSF Research Center, Germany). GFP-PES1 was constructed in our laboratory. The PES1 mutants GFP-K249R, K517R, and 2KR were prepared using the QuikChange Site-Directed Mutagenesis Kit (Stratagene, La Jolla, CA) using HA-PES1 as the template. Myc-tagged SUMO-1, SUMO-2, SUMO-3 along with GFP-SUMO1 and its mutant GFP-SUMO1/GA have been used in our previous studies [11 (link), 12 (link)]. Flag-UBC9 was amplified by PCR and subcloned into Flag-pCDNA3.1 vector at Xho1 and EcoR1 sites.

Li S., Wang M., Qu X., Xu Z., Yang Y., Su Q, & Wu H. (2016). SUMOylation of PES1 upregulates its stability and function via inhibiting its ubiquitination. Oncotarget, 7(31), 50522-50534.

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Immunofluorescence Assay for Protein Localization

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Immunofluorescence experiments were completed as described in (Bolt et al., 2013 (link)). Briefly, cells were fixed in 4%
formaldehyde in PEM buffer (80 mM potassium PIPES, pH 6.8, 5 mM EGTA and 2 mM
MgCl₂), quenched with 0.1 M ammonium chloride for 10 min and permeabilized
with 0.5% Triton X-100 for 30 minutes. Cells were incubated at room temperature in blotto
(5% milk in 1× TBS–Tween 20) for 1 h, and then specific antibodies were
added overnight at 4°C prior to 30 min of secondary antibody (Alexa conjugates,
Molecular Probes) and DAPI staining (1μg/ml for ~1 min). The primary
antibodies used were: mouse anti-Ser5-phospho RNA polymerase II (Abcam, ab5401), mouse
anti-SRC-1 (BD Transduction Labs # 612378), mouse anti-SRC-2 (BD Transduction Labs #
610985), mouse anti-SRC-3 (BD Transduction Labs # 611105), and rabbit anti-ERα
(Millipore 04-820).

Stossi F., Bolt M.J., Ashcroft F.J., Lamerdin J.E., Melnick J.S., Powell R.T., Dandekar R.D., Mancini M.G., Walker C.L., Westwick J.K, & Mancini M.A. (2014). Defining Estrogenic Mechanisms of Bisphenol A Analogs through High Throughput Microscopy-based Contextual Assays. Chemistry & biology, 21(6), 743-753.

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Immunohistochemical Localization of Steroid Receptors in Murine Brain

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Brain sections from male mice were washed with PBS and blocked for 1 hr at room temperature with 2% bovine serum albumin (BSA) containing 0.3% Triton X-100 in PBS. They were then incubated for 48 hr at 4°C with the following primary antibodies: rabbit anti-AR (1:200; Epitomics) or rabbit anti-ERα (1:5000; Millipore Corp) and goat anti-choline acetyltransferase (ChAT) (1:1000; Millipore Corp) or mouse anti-calcitonin gene-related peptide (CGRP) (1:8000; Abcam, Cambridge, UK). After the primary antibody was removed by rinsing, sections were incubated with secondary antibodies for 1 hr at room temperature. The following secondary antibodies were used for double-staining: anti-rabbit Alexa Fluor (AF) 488 (1:1000; Invitrogen, Grand Island, NY, USA) and anti-goat AF 546 (1:1000; Invitrogen) or anti-mouse AF 546 (1:1000; Invitrogen).

Mukudai S., Ichi Matsuda K., Bando H., Takanami K., Nishio T., Sugiyama Y., Hisa Y, & Kawata M. (2016). Expression of Sex Steroid Hormone Receptors in Vagal Motor Neurons Innervating the Trachea and Esophagus in Mouse. Acta Histochemica et Cytochemica, 49(1), 37-46.

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Investigating Neuropeptide Y Neuron Co-Expression

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To investigate NPY^ARN co-expression with gonadal steroid hormone receptors, free-floating immunohistochemistry was performed on coronal sections throughout the rostral, middle, and caudal ARN (rARN, mARN, cARN). NPY^ARN neuron somata were labeled for GFP reporter expression using a chicken anti-GFP primary antibody (1:5000; Aves Labs) [45 (link)] as above. Every third section was co-labeled for one of the following steroid hormone receptors: progesterone receptor (rabbit anti-PR, 1:100; Dako (Agilent), CA, USA; VEH n = 8, PNA n = 10; RRID:AB_2315192) [46 (link)], estrogen receptor α (rabbit anti-ERα, 1:5000; Millipore; VEH n = 5, PNA n = 5, RRID:AB_310305) [47 (link)], or androgen receptor (rabbit anti-AR PG-21, 1:200; Millipore, MA, USA; VEH n = 4, PNA n = 4; RRID:AB_310214) [48 (link)]. To amplify GFP signal, a goat anti-chicken AlexaFluor488 antibody was used (1:200; Molecular Probes, OR, USA), while steroid hormone receptors were labeled using a goat anti-rabbit AlexaFluor568 antibody (1:200; Molecular Probes, OR, USA).

Marshall C.J., Prescott M, & Campbell R.E. (2020). Investigating the NPY/AgRP/GABA to GnRH Neuron Circuit in Prenatally Androgenized PCOS-Like Mice. Journal of the Endocrine Society, 4(11), bvaa129.

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Immunohistochemical Analysis of Brain Sections

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Brain sections from male and female mice were used in this experiment. Endogenous peroxidase activity was eliminated from the sections by incubation with 2% H₂O₂ in PBS with 0.3% Triton X-100 for 30 min, and the sections were then rinsed with PBS three times for 5 min each. After blocking nonspecific binding components with 1% normal goat serum and 1% BSA in PBS containing 0.1% Triton X-100 for 1 hr at room temperature, the sections were incubated with rabbit anti-AR (1:200; Epitomics, Burlingame, CA, USA) or rabbit anti-ERα (1:5000; Millipore Corp, Billerica, MA, USA) antibodies for 48 hr at 4°C. Immunopositive products were detected with a streptavidin-biotin (SAB) kit (Nichirei, Tokyo, Japan), followed by diaminobenzidine (DAB) development. The preparations were analyzed using an Olympus Optical microscope (Tokyo, Japan).

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Tracing Tracheal and Esophageal Innervation

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Male mice were anesthetized with isoflurane. The extrathoracic trachea and esophagus were exposed and a single injection (200 nl) of a 2% solution of cholera toxin B subunit (CTb) conjugated to AF 488 (CTb 488, Molecular Probes, Eugene, OR, USA) was then injected into the tracheal adventitia or/and (in the same animal) a single injection (200 nl) of a 2% solution of CTb conjugated to AF 555 (CTb 555, Molecular Probes) was injected into the cervical esophagus wall between the muscularis and mucosal layers through a glass micropipette [29 , 35 (link)]. Great care was taken to prevent unwanted spread of dye to neighboring tissues. After 5 days, animals were perfusion fixed and tissue was processed as described below. Coronal sections of 20 μm were washed with PBS and blocked for 1 hr at room temperature with 2% BSA containing 0.3% Triton X-100 in PBS. They were then incubated with goat anti-ChAT (1:1000; Millipore Corp.), rabbit anti-AR (1:200; Epitomics) or rabbit anti-ERα (1:5000; Millipore Corp) primary antibodies for 48 hr at 4°C. After the primary antibody was removed by rinsing, sections were incubated with anti-goat or rabbit AF 633 (1:1000; Invitrogen) secondary antibody for 1 hr at room temperature.

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