Costar ultra low attachment u bottom 96 well plates

Manufactured by Corning

Costar Ultra low attachment U-bottom 96-well plates are used for cell culture applications. The plates feature a U-shaped bottom design and are made with a specialized surface treatment to minimize cell attachment.

Automatically generated - may contain errors

Lab products found in correlation

Celigo imaging cytometer, by Revvity (2 mentions)

Close spelling variants of this product

Ultra-low-attachment 96-well U-bottom plates Ultra-low attachment U-bottom 96-well plate Costar® Ultra-Low Attachment 96-well plate Low attachment 96-well U-bottom plate U-bottom ultra-low attachment plates Ultra-low attachment 96-well plates Costar ultra-low attachment plates Well ultra-low attachment plates Low-attachment 96-well plates 96-well U-bottom plates

2 protocols using costar ultra low attachment u bottom 96 well plates

3D Spheroid Formation and Imaging

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

Spheroids were formed as previously described [60 (link)]. Briefly, cells were suspended in Perfecta 3D hanging drop plates (HDP1096, 3D Biomatrix, MI) at 25,000 cells/40 µl droplet. Spheroids were allowed to form for 5 days and then centrifuged at 450 rpm, with no brake, into media-containing Costar Ultra low attachment U-bottom 96-well plates (CLS3474, Corning, NY). Spheroids were imaged in the Celigo Imaging Cytometer (Nexcelom Bioscience, MA) using bright-field imaging single colony verification analysis.

Russell S., Xu L., Kam Y., Abrahams D., Ordway B., Lopez A.S., Bui M.M., Johnson J., Epstein T., Ruiz E., Lloyd M.C., Swietach P., Verduzco D., Wojtkowiak J, & Gillies R.J. (2022). Proton export upregulates aerobic glycolysis. BMC Biology, 20, 163.

+ Open protocol

+ Expand

Formation and Imaging of 3D Spheroids

Check if the same lab product or an alternative is used in the 5 most similar protocols

Spheroids were formed as previously described (Russell et al., 2017) . Briefly, cells were suspended in Perfecta 3D hanging drop plates (HDP1096, 3D Biomatrix, MI) at 25K cells/40ul droplet. Spheroids were allowed to form for 5 days and then centrifuged at 450rpm, with no brake, into media-containing Costar Ultra low attachment U-bottom 96-well plates (CLS3474, Corning, NY). Spheroids were imaged in the Celigo Imaging Cytometer (Nexcelom Bioscience, MA) using bright field imaging single colony verification analysis.

Russell S., Xu L., Kam Y., Abrahams D., Verduzco D., Johnson J., Epstein T., Ruiz E., Lloyd M.C., Wojtkowiak J.W., Lopez A.S., Bui M.M., Gillies R.J., Swietach P., & Ordway B. (2021). Proton export drives the Warburg Effect.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!