The Illumina HumanOmniExpress-FFPE BeadChip assay was used to analyze the genotype, DNA copy number, and loss of heterozygosity (LOH) in 48 primary-normal paired samples. All samples, except for 1 (n = 47), passed our quality control metrics for sample identity and data quality. A subset of 693,000 high-quality single nucleotide polymorphisms was selected for all analyses (Supplemental Figure 1, Supplemental Digital Content 2,
Absolutely rna ffpe kit
The Absolutely RNA FFPE Kit is a laboratory equipment product designed for the extraction and purification of total RNA from formalin-fixed, paraffin-embedded (FFPE) tissue samples. The kit provides the necessary reagents and protocols to efficiently isolate high-quality RNA from FFPE samples for downstream applications.
Lab products found in correlation
8 protocols using absolutely rna ffpe kit
Exome Sequencing and Genotyping for NSCLC
The Illumina HumanOmniExpress-FFPE BeadChip assay was used to analyze the genotype, DNA copy number, and loss of heterozygosity (LOH) in 48 primary-normal paired samples. All samples, except for 1 (n = 47), passed our quality control metrics for sample identity and data quality. A subset of 693,000 high-quality single nucleotide polymorphisms was selected for all analyses (Supplemental Figure 1, Supplemental Digital Content 2,
Complementary DNA Synthesis from RNA
All RT reactions included one no-template negative control. Moreover, 1 µL containing 1 µg of the QPCR Human Reference Total RNA, part of the Absolutely RNA FFPE Kit (Agilent, Santa Clara, CA, USA), was used as a positive control to monitor the quality of the RT.
Tumor Cell RNA Extraction from FFPE
GC Mucosa Transcriptome Analysis
Complementary DNA (cDNA) was synthesized from up to 2 µg of RNA using the High-Capacity cDNA Reverse Transcription (RT) kit (Thermo Fisher Scientific, Waltham, MA, USA) following the manufacturer’s instructions.
All RT reactions included one no-template negative control. Moreover, 1 µL containing 1 µg of the QPCR Human Reference Total RNA, part of the Absolutely RNA FFPE Kit (Agilent, Santa Clara, CA, USA), was used as a positive control to monitor the quality of the RT.
RNA Isolation and qPCR for FPN1 Expression
CD44 Isoform Expression Analysis
Intracellular ROS Detection via H2DCF-DA
At the end of the incubation time H2DCF-DA 20 μM were added and cells were incubated for 45 minutes at 37°C.The uorescence signal was quanti ed using a microplate uorescence reader (VictorXTM4 microplate reader, PerkinElmer Inc, Waltham, Ma, USA), using 485 nm as excitation and 535 nm as emission wavelength.
mRNA analysis in real time PCR Total cytoplasmic RNA from cell lines was extracted using the TRIzol reagent protocol and RNA from FFPE tissues was extracted using the Absolutely RNA FFPE Kit (Agilent, CA, USA) using the manufacturer's instructions. RNA concentration was measured using the Qubit™ RNA HS Assay Kit (Thermo Fisher Scienti c). Aliquots (1 µg) of RNA were converted to cDNA using random hexamer primers. Quantitative changes in mRNA levels were estimated by real time PCR using the following cycling conditions: 35
Verification of Novel Fusion Transcripts
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!