Unless indicated, all chemicals and drugs were from Sigma-Aldrich/Merck Millipore. Primary antibodies used in this study included the following: Rabbit anti-HA clone C29F4, anti-pSrc Tyr416 clones 100F9 and D49G4, anti-Na+/K+ ATPase #3010, and anti-GFP clone D5.1 (Cell Signaling Technology); rabbit Anti-APP clone Y188 (Abcam); mouse anti-HA clone 16B12 (Covance); rabbit anti-actin #AAN01 (Cytoskeleton Inc); anti-transferrin receptor clone H68.4 (Thermo Fisher Scientific); rabbit anti-methylated Lysine (Enzo Life Sciences); mouse anti-Fyn clone 25 #610163, anti-flotillin-1 clone 18, and anti-PP2Acα clone 46 (BD Transduction Laboratories); rabbit anti-Fyn clone EPR5500, mouse anti-Bα clone 2G9, anti-actin clone C4, anti-LCMT1 clone 4A4, anti-APP clone 22C11 (MAB348), and anti-demethyl PP2Ac clone 1D6 (Merck Millipore).
Rabbit anti fyn clone epr5500
Manufactured by Merck Group
Rabbit anti-Fyn clone EPR5500 is a primary antibody that specifically recognizes the Fyn protein, a member of the Src family of tyrosine kinases. This antibody can be used for the detection and analysis of Fyn expression in various biological samples.
Automatically generated - may contain errors
Lab products found in correlation
Rabbit anti ha clone c29f4, by Cell Signaling Technology (2 mentions)
Mouse anti bα clone 2g9, by Merck Group (2 mentions)
Mouse anti ha clone 16b12, by Fortrea (2 mentions)
Mouse anti p44 42 mapk erk1 2 l34f12, by Cell Signaling Technology (2 mentions)
Mouse anti gsk3β clone 3d10, by Cell Signaling Technology (2 mentions)
Rabbit anti phospho sfk y416 clone 100f9, by Cell Signaling Technology (2 mentions)
Anti transferrin receptor clone h68, by Thermo Fisher Scientific (1 mentions)
Anti actin clone c4, by Merck Group (1 mentions)
Ab108387, by Abcam (1 mentions)
Rabbit anti phosphogsk3β ser9 d85e12, by Cell Signaling Technology (1 mentions)
Mouse anti actin clone c4, by Merck Group (1 mentions)
Rabbit anti gfp clone d5, by Cell Signaling Technology (1 mentions)
Rabbit anti phospho gsk 3β ser9, by Cell Signaling Technology (1 mentions)
Clone c4, by Merck Group (1 mentions)
3 protocols using rabbit anti fyn clone epr5500
1
Antibody Characterization for Protein Studies
2
Molecular Mechanisms of Tau Hyperphosphorylation
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Unless indicated, all chemicals were purchased from Sigma/Merck Millipore. Antibodies used for immunoblotting included: mouse anti‐demethylated PP2Ac (clone 1D6, Merck Millipore); mouse anti‐PP2Acα/β (clone 46, BD Transduction); rabbit anti‐phospho‐p44/42 mitogen activated protein kinase (MAPK) (#9101, Cell Signaling Technology); mouse anti‐p44/42 MAPK (ERK1/2) (L34F12, Cell Signaling Technology); rabbit anti‐phospho‐GSK3β (Ser9) (D85E12, Cell Signaling Technology); mouse anti‐GSK3β (clone 3D10, Cell Signaling Technology); rabbit anti‐Tau (T‐1308‐1, rPeptide); mouse anti‐pS202‐Tau (CP13) [41 (link)] and anti‐pSer396/Ser404 (PHF‐1) tau (kindly donated by Peter Davies); mouse anti‐pS422‐tau (44‐764G, ThermoFisher); mouse anti‐human Tau‐13 (ab19030, Abcam); rabbit anti‐Fyn (clone EPR5500, Merck Millipore); mouse anti‐Fyn clone 25 (BD Transduction Laboratories); rabbit anti‐phospho‐SFK (Y416) (clone 100F9, Cell Signaling Technology); mouse anti‐actin (clone C4, Merck Millipore); and mouse anti‐glyceraldehyde 3‐phosphate dehydrogenase (clone 6C5, Merck Millipore). Mouse anti‐Fyn (clone 15, Santa Cruz), rabbit anti‐pS202‐Tau (ab108387, Abcam) and PHF‐1 antibodies were used for immunostaining.
3
Antibody-Based Analysis of Protein Signaling
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Unless indicated, all chemicals were purchased from Sigma/Merck Millipore. Antibodies used in this study included mouse anti-HA (clone 16B12, Covance); rabbit anti-HA (clone C29F4, Cell Signaling Technology); rabbit anti-GFP (clone D5.1, Cell Signaling Technology); mouse anti-Bα (clone 2G9, Merck Millipore); mouse anti-PP2Acα/β (BD Transduction); goat anti-pY307 PP2Ac (Santa Cruz Biotechnology, Inc; denoted SC); rabbit anti-phospho-SFK (Y416) (clone 100F9, Cell Signaling Technology); rabbit anti-phospho-p44/42 MAPK (clone D13.14.4E, Cell Signaling Technology); mouse anti-p44/42 MAPK (ERK1/2) (L34F12, Cell Signaling Technology); rabbit anti-phospho-GSK-3β (Ser 9) (Cell Signaling Technology) and mouse anti-GSK3β (clone 3D10, Cell Signaling Technology); rabbit anti-Src (clone 32G6, Cell Signaling Technology); mouse anti-Src (clone GD11, Merck Millipore); rabbit anti-Fyn (clone EPR5500, Merck Millipore); mouse anti-Fyn (BD Transduction and Cell Signaling Technology); mouse and rabbit anti-p-Tyr (P-Tyr-100 and P-Tyr-1000, Cell Signaling Technology); rabbit anti-Tau (rPeptide), mouse anti-pSer202 Tau (CP13) (59 (link)) (a gift from Peter Davies). Mouse (clone C4, Merck Millipore) or rabbit (Cytoskeleton) anti-actin, and mouse (clone 236-10501, Life Technologies Australia Pty Ltd) or rabbit (Epitomics) anti-α-tubulin antibodies were used for protein normalization.
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