Rabbit anti cyclin b1

Manufactured by Santa Cruz Biotechnology

Sourced in United States

Rabbit anti-cyclin B1 is a primary antibody that specifically recognizes the cyclin B1 protein. Cyclin B1 is a key regulator of the cell cycle and plays a crucial role in the G2/M transition. This antibody can be used for the detection and analysis of cyclin B1 in various experimental applications.

Automatically generated - may contain errors

Lab products found in correlation

4 protocols using rabbit anti cyclin b1

Protein Immunoblotting Assay Protocol

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

Cell pellets were lysed as described previously (19 (link)). Primary antibodies that we used in this study were rabbit anti-phospho Aurora A (T288), rabbit anti-insulin-like growth factor-I receptor (IGF- IR), rabbit anti-insulin receptor (InsR) β, rabbit anti-phospho-IGF-IR/InsR kinase (Thr202/Tyr204), rabbit anti–extracellular signal-regulated protein kinases (ERKs), rabbit anti–phospho-ERKs, rabbit anti–c-Jun N-terminal kinases (JNKs), rabbit anti–phospho-JNKs, rabbit anti–p38, and rabbit anti–phospho-p38 (all from Cell Signaling Technology, Beverly, MA); and mouse anti-Aurora A (BD Biosciences, San Jose, CA), rabbit anti-cyclin B1 (Santa Cruz Biotechnology, Dallas, TX), and mouse anti–β-actin Ab (diluted at 1: 5000; Sigma-Aldrich, St. Louis, MO). Antibodies were diluted at a ratio of 1:1000, unless noted. Signals were detected with use of an Odyssey IR imaging system (LI-COR, Lincoln, NE).

Kai K., Kondo K., Wang X., Xie X., Pitner M.K., Reyes M.E., Torres-Adorno A.M., Masuda H., Hortobagyi G.N., Bartholomeusz C., Saya H., Tripathy D., Sen S, & Ueno N.T. (2015). Antitumor Activity of KW-2450 Against Triple-Negative Breast Cancer by Inhibiting Aurora A and B Kinases. Molecular cancer therapeutics, 14(12), 2687-2699.

+ Open protocol

+ Expand

SDS-PAGE and Western Blot Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols

Co-immunoprecipitation samples were subjected to SDS-PAGE on acrylamide gel. For Commassie blue staining, the acrylamide gel was stained with Brilliant Blue R250 Protein solution (Elpis, Korea) for 1 h with gentle agitation and then washed three times with destaining solution containing 20% methanol and 10% acetic acid for 1 h. For Western blotting, SDS-PAGE gel was transferred onto PVDF membranes (Millipore). After blocking with 5% skimmed milk in TBS-T buffer (50 mM Tris-HCl, pH 7.4, 150 mM NaCl, 0.1% Tween20) for 1 h, membranes were incubated with the primary antibodies at 4°C with shaking overnight. After incubated with the secondary antibody for 2 h, immunocomplexes were detected by use of the femto, Pico or ECL reagent (Thermo). Antibodies for Western blotting are from the companies as follows: mouse/rabbit anti-flag antibody (Sigma), mouse anti-VRK1 (Santa Cruz), rabbit anti-VRK3 (Sigma), mouse ant-ALY antibody (Santa Cruz), rabbit anti-hsp70 (Origene), rabbit anti-XRCC5 (Santa Cruz), rabbit anti-B23 antibody (Santa Cruz), rabbit anti-C23 antibody (Santa Cruz), rabbit anti-PARP1 antibody (Cell signaling), rabbit anti-cyclin B1 (Santa Cruz), rabbit anti-p53 (Cell signaling) and rabbit anti-BAF [previously used (Kim et al., 2015 (link))].

Lee N., Kim D.K., Han S.H., Ryu H.G., Park S.J., Kim K.T, & Choi K.Y. (2017). Comparative Interactomes of VRK1 and VRK3 with Their Distinct Roles in the Cell Cycle of Liver Cancer. Molecules and Cells, 40(9), 621-631.

+ Open protocol

+ Expand

Immunoblotting Antibody Evaluation Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols

Chemicals were purchased from Sigma (St Louis, MO, USA) unless indicated otherwise. Antibodies against GAPDH and α-tubulin were purchased from Sigma. The mouse anti-Cyclin B1 and the rabbit anti-ERLIN2 antibodies were from Cell Signaling (Beverly, MA, USA). Antibodies against V5 were from Invitrogen (Grand Island, NY, USA). NeuN antibody was from EMD Millipore (Billerica, MA, USA). Acetylated-α-tubulin was from Abcam (Cambridge, MA, USA). The rabbit anti-Cyclin B1, mouse anti-HA and mouse anti-Cdk1 antibodies were purchased from Santa Cruz Biotechnology (Dallas, TX, USA). The mouse anti-Cdc27 antibody was from BD transduction Laboratary (San Jose, CA, USA). Bortezomib (B-1408) was from LC Laboratories (Woburn, MA, USA).

Zhang X., Cai J., Zheng Z., Polin L., Lin Z., Dandekar A., Li L., Sun F., Finley RL J.r., Fang D., Yang Z.Q, & Zhang K. (2015). A novel ER–microtubule-binding protein, ERLIN2, stabilizes Cyclin B1 and regulates cell cycle progression. Cell Discovery, 1, 15024-.

+ Open protocol

+ Expand

Immunostaining and Western Blot Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols

The following antibodies were used for immunostaining; Rabbit anti-CtIP (D76F7, #9201, cell signaling, Danvers, MA, USA), mouse anti-CtIP (D-4, 271339, Santa Cruz Biotechnology, Dallas, TX, USA), rabbit anti-TPX2 (NB500-179, NOVUS, Centennial, CO, USA), rabbit anti-γ tubulin (T3559, Sigma, St Louis, MO, USA), mouse anti-α-tubulin (#14-4502-82, Invitrogen), rabbit anti-human ANA-Centromere CREST (90C-CS1058, Fitzgerald, Wicklow, Ireland), mouse anti-BubR1 (ab4637, abcam, Cambridge, UK), rabbit anti-Mad1 (sc-67338, Santa Cruz Biotechnology), rabbit anti-Mad2 (A300-300A, Bethly, Fortis life sciences, Waltham, MA, USA), rabbit anti-phospho Aurora A T288 (3079, Cell Signaling, Danvers, MA, USA), mouse anti-RCC1 (F-2, Santa Cruz Biotechnology). The following antibodies were used for Western blot analysis; mouse anti-CtIP (D-4, 271339, Santa Cruz Biotechnology), rabbit anti-TPX2 (NB500-179, NOVUS), rabbit anti-CyclinB1 (H-433, Santa Cruz Biotechnology), rabbit anti-Histone H3 (06-755, Millipore, Burlington, MA, USA), rabbit anti-phospho-Histone H3 Ser10 (9701, Cell Signaling), mouse anti-β-actin (sc-47778, Santa Cruz Biotechnology), mouse anti-Flag (M2, Sigma) and mouse anti-HA (F-7, Santa Cruz Biotechnology). For Immunoprecipitation assay, mouse anti-Flag (M2, Sigma) and rabbit anti-HA (600-401-384, Rockland, Limerick, PA, USA) were used.

Oh W., Wu T.T., Jeong S.Y., You H.J, & Lee J.H. (2022). CtIP Regulates Mitotic Spindle Assembly by Modulating the TPX2-Aurora A Signaling Axis. Cells, 11(18), 2814.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!